BBSRC-NSF/BIO: Collaborative Research: Focusing a quantitative lens on Synthetic Phototrophic Communities

BBSRC-NSF/BIO：合作研究：将定量视角聚焦于合成光养群落

• 批准号: 2105796
• 负责人: Seppe Kuehn
• 金额: $ 33.19万
• 依托单位: University of Chicago
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-01-15 至 2024-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2105796&HistoricalAwards=false
• 关键词: BBSRC; NSF; BIO; Collaborative; Research

Microbial communities populate nearly all environments on our planet. These environments include the human gut, the surface of corals in the oceans, and plant roots in the soil. In these communities, bacteria interact with each other to sustain environmental health. Understanding these interactions will reveal factors that establish robust, productive communities. This project will focus on bacteria isolated from hot spring mats of Yellowstone National Park. A deeper understanding of the physical and metabolic conversations among bacteria that thrive in this extreme environment will be gained and used to model these interactions. New courses on microbial consortia will be developed and shared by all participating Universities. The PIs will also develop programs in partnership with local science museums and TV stations to encourage science literacy and community involvement. This project is focused on extremophile bacteria isolated from the hot spring mats of Yellowstone National Park. The approach will be to query the entire bacterial community within the mat as well as synthetic combinations of the bacteria grown under defined conditions in the laboratory. The application of sequencing and metabolomic technologies will reveal key metabolic pathways among members of the community under different light and nutrient conditions and over the diel cycle. The work will evaluate the hypothesis that microbial communities act like an integrated machine rather than as individual species grown under axenic conditions in the laboratory, and that this integration maintains functionalities critical for life in natural habitats. Experiments will also be performed to analyze how abrupt changes in light intensity, temperature and microbial composition impact mat growth, structure, electrical and metabolic networks.This collaborative US/UK project is supported by the US National Science Foundation and the UK Biotechnology and Biological Sciences Research CouncilThis award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

微生物群落几乎遍布我们地球上的所有环境。这些环境包括人类肠道，海洋中的珊瑚表面以及土壤中的植物根。在这些社区中，细菌相互互动以维持环境健康。了解这些相互作用将揭示建立强大而有效社区的因素。 该项目将重点放在黄石国家公园温泉垫中分离出的细菌。对在这种极端环境中壮成长的细菌之间的物理和代谢对话的更深入了解将获得并用来对这些相互作用进行建模。所有参与大学将开发和共享有关微生物财团的新课程。 PI还将与当地科学博物馆和电视台合作制定计划，以鼓励科学素养和社区参与。 该项目的重点是从黄石国家公园的温泉垫中分离出来的极端细菌。该方法将是查询MAT内的整个细菌群落以及在实验室定义条件下生长的细菌的合成组合。测序和代谢组技术的应用将在不同的光和营养条件下以及在DIEL周期内揭示社区成员之间的关键代谢途径。这项工作将评估以下假设：微生物群落像一台综合机器一样，而不是在实验室的轴承条件下生长的单个物种，并且这种整合保持对自然栖息地中生命至关重要的功能。还将进行实验，以分析光强度，温度和微生物组成的突然变化影响MAT的增长，结构，电气和代谢网络。该协作美国/英国项目得到了美国国家科学基金会和英国生物技术和生物学科学研究的支持 标准。