EAGER: Towards establishing trait-based phenotypes for Symbiodiniaceae algae within reef corals - A low-cost approach using an open-source instrument platform.

EAGER：为珊瑚礁珊瑚内的共生科藻类建立基于性状的表型 - 使用开源仪器平台的低成本方法。

• 批准号: 2054885
• 负责人: Kenneth Hoadley
• 金额: $ 29.02万
• 依托单位: University of Alabama Tuscaloosa
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-03-01 至 2025-02-28
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2054885&HistoricalAwards=false
• 关键词: EAGER; Towards; establishing; trait; based

As worldwide coral bleaching events continue to increase in severity and frequency, new techniques are desperately needed to help us better understand how individual Symbiodiniaceae species are functionally different from one another and how this contributes to the current patterns of thermal susceptibility and tolerance observed across reef systems. The PI’s newly developed prototype instrument would enable researchers to exploit differences in the relative abundance and use of photopigments, and their response to fluctuating light to resolve species-specific functional traits within algae. The need for standardized tools that help predict bleaching vulnerability and improve our understanding of thermal tolerance was highlighted in a NSF sponsored coral bleaching workshop held in July of 2016.The PIs request funding use the PI’s prototype fluorometer to assess photo-physiological differences across over 30 different species of coral currently grown within land-based nurseries. Production of a large data set which matches phenotype to genotype is critical for ground truthing the instruments ability to resolve functional differences across Symbiodiniaceae species. The PI will also further optimize the instrument for underwater deployment so that coral/algal symbioses may be queried in-situ. The end product would be a highly versatile and low-cost instrument platform readily accessible to both researchers and conservation/restoration focused groups.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

随着全球珊瑚漂白事件的严重程度和频率继续增加，迫切需要新技术，以帮助我们更好地了解单个共生性种类在功能上如何与彼此的功能不同，这如何有助于当前在整个礁系统中观察到的热敏感和耐受性的模式。 PI新开发的原型仪器将使研究人员能够探索相对丰度和使用光的差异，以及它们对波动光的反应以解决藻类内的规格特异性功能性状。在2016年7月举行的NSF赞助的珊瑚漂白研讨会中，强调了有助于预测漂白脆弱性并提高我们对热耐受性的理解的标准化工具的需求。将表型与基因型匹配的大数据集的产生对于培养仪器能够解决共生性种类的功能差异的能力至关重要。 PI还将进一步优化水下部署的仪器，以便可以在原位查询珊瑚/藻类符号。最终产品将是一个非常通用且低成本的仪器平台，研究人员和以保护/恢复为重用的组都可以访问。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子和更广泛的影响评估标准来评估NSF的法定任务。

项目成果

Bio-optical signatures of in situ photosymbionts predict bleaching severity prior to thermal stress in the Caribbean coral species Acropora palmata

原位光共生体的生物光学特征预测加勒比珊瑚物种鹿角珊瑚在热应激之前的漂白严重程度

• DOI: 10.1007/s00338-023-02458-5
• 发表时间: 2024
• 期刊: Coral Reefs
• 影响因子: 3.5
• 作者: Hoadley, Kenneth D.;Lowry, Sean;McQuagge, Audrey;Dalessandri, Shannon;Lockridge, Grant;O’Donnell, Sibelle;Elder, Holland;Ruggeri, Maria;Karabelas, Eleftherios;Klepac, Courtney
• 通讯作者: Klepac, Courtney

A phenomic modeling approach for using chlorophyll-a fluorescence-based measurements on coral photosymbionts

• DOI: 10.3389/fmars.2023.1092202
• 发表时间: 2023-03-02
• 期刊: FRONTIERS IN MARINE SCIENCE
• 影响因子: 3.7
• 作者: Hoadley, Kenneth D.;Lockridge, Grant;Muller, Erinn M.
• 通讯作者: Muller, Erinn M.