I-Corps: Cell Culture Platform Using Engineered Micropatterns to Control Differentiation

I-Corps：使用工程微图案控制分化的细胞培养平台

• 批准号: 2037874
• 负责人: Scott Wood
• 金额: $ 5万
• 依托单位: South Dakota School of Mines and Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-01 至 2023-01-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2037874&HistoricalAwards=false
• 关键词: Corps; Cell; Culture; Platform; Using

The broader impact/commercial potential of this I-Corps project is the development of an in vitro, articular cartilage model for osteoarthritis (OA). This model is designed to increase the likelihood of successful clinical translation of research on the causes of OA into potential treatments. OA often occurs with age or injury and affects over 32.5 million US adults. There are currently no known disease-modifying treatments to stop or reverse the progression of OA – all current treatments are limited to either various methods of pain management or surgical tissue replacement. The proposed technology will advance the translation of OA research by facilitating studies of chondrocytes, the primary cells that cause OA, using traditional and state-of-the-art genomic, proteomic, and imaging techniques. It is expected that the proposed technology will bridge the gap between the 2D and 3D cell culture markets, represented by an annual market of ~$1 billion in the US. Due to the prevalence and crippling nature of OA, joint replacements represent a $19 billion industry annually in the US. In addition to its role in OA treatments, the model may clinical implications in improving outcomes for autologous chondrocyte transplantation, increasing its commercial impact.This I-Corps project is based on the development of a cell culture platform that improves control over the differentiation of chondrocytes. This control is enabled through the regulation of cell shape via a novel combination of nanotechnology, micropatterning, and mechanically-tunable, thin-film composite materials. Chondrocytes rapidly transform into non-physiological cell types in standard 2D culture systems. More advanced 3D culture systems prevent this problem but introduce difficulties in compatibility with analytical techniques. The proposed technology may act as an egg crate for individual cells, nesting each one in an environment that allows it to maintain its physiological nature without restricting their ability to be studied. The technology may maintain the physiological cell shape of chondrocytes for at least 28 days, four times as long as competing micropatterned technologies. The technology has potential applications in drug development, gene therapy, stem cell medicine, tissue engineering, the elucidation of molecular pathogeneses, and other biomedical applications.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该I-Corps项目的更广泛的影响/商业潜力是开发用于骨关节炎（OA）的体外关节软骨模型。该模型旨在增加对OA原因研究为潜在治疗的成功临床翻译的可能性。 OA通常会随着年龄或伤害而发生，并影响超过3250万美国成年人。目前尚无已知的疾病修改治疗方法可以阻止或扭转OA的进展 - 所有当前治疗都仅限于各种疼痛管理方法或手术组织置换方法。提出的技术将通过支持软骨细胞的研究，即使用传统和最先进的基因组，蛋白质组学和成像技术来推动OA研究的翻译。预计拟议的技术将弥合2D和3D细胞培养市场之间的差距，该市场的年度市场约为10亿美元。由于OA的普遍性和严重的性质，联合替代者每年在美国代表190亿美元的行业。除了其在OA处理中的作用外，该模型还可能在改善自体软骨细胞移植的结果方面具有临床意义，从而增加其商业影响。这个I-Corps项目基于一个细胞培养平台的开发，该平台改善了对软骨细胞的分化的控制。通过通过纳米技术，微图和机械可调的薄膜复合材料的新组合来调节细胞形状，可以通过调节细胞形状来实现此控制。软骨细胞在标准2D培养系统中迅速转化为非生理细胞类型。更高级的3D培养系统可以防止此问题，但在与分析技术的兼容性方面引入了困难。提出的技术可以充当单个细胞的卵箱，将每个细胞嵌套在环境中，使其能够保持其物理性质，而无需限制其被研究的能力。该技术可以将软骨细胞的物理细胞形状保持至少28天，这是竞争微图案技术的四倍。该技术在药物开发，基因治疗，干细胞医学，组织工程，阐明分子病原体和其他生物医学应用中具有潜在的应用。该奖项反映了NSF的法定任务，并通过使用基金会的知识分子和更广泛的影响审查标准来通过评估来诚实地支持。