RAPID: Development and Testing of Low-Cost Sensor Platforms for SARS-CoV-2 in Aerosols

RAPID：气溶胶中 SARS-CoV-2 低成本传感器平台的开发和测试

• 批准号: 2029911
• 负责人: Peter Vikesland
• 金额: $ 20万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-15 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2029911&HistoricalAwards=false
• 关键词: RAPID; Development; Testing; Low; Cost

The COVID-19 pandemic has impacted human health on a global scale, with over a quarter million fatalities to date. There is growing evidence that COVID-19 is transmitted by the presence of SARS-COV2 virus in small respiratory droplets known as aerosols. To slow transmission of COVID-19, we need a better understanding of how much virus travels through the air in these droplets. Current methods to measure the virus are too time consuming to provide information necessary to fully combat transmission through air. Using nanotechnology, the proposed research will develop a faster way to measure viruses in small droplets. This will allow us to measure the virus in more locations to track them as they move through indoor air. The project team leverages research expertise in nanotechnology, aerosol science, and viral detection. Successful completion of this project will lead to production of a more rapid COVID-19 analysis tool that does not require RNA extraction and can be used for rapid and inexpensive testing. Results will be used to evaluate the scientific validity of the “six-foot rule” recommended for social distancing.There is a pressing need for rapid and sensitive environmental detection of the respiratory virus SARS-CoV2, the agent responsible for the COVID-19 pandemic. Most current approaches for SARS-CoV2 detection rely upon RT-qPCR for quantification of viral RNA. These techniques are both labor and cost intensive. The goal of this research is to develop a rapid SARS-CoV2 detection method to test the hypothesis that SARS-CoV2 is spread by aerosols. A second goal is to assess whether the recommended six-foot social distancing guideline is scientifically valid, as research supporting this recommendation is sparse. These goals will be achieved through the development of a low-cost sensor platform for the rapid detection of aerosolized SARS-CoV2 virus that uses surface enhanced Raman spectroscopy (SERS) to detect viruses in under an hour. The secondary objective of this study is to deploy the developed platform within the built environment to quantify SARS-CoV2 transport. The project team includes researchers with complimentary expertise in nanosensor development, bioaerosol characterization, and virus detection. In the development of the sensor platform we will examine virus recovery and detection using both plastic and fabric coupons as well as gold-nanocellulose and gold-adhesive tape SERS substrates. Transport of aerosolized SARS-CoV2 in the built environment will be assessed using Phi6 (a surrogate for SARS-Cov2) and heat-inactivated SARS-CoV2 nebulized to generate aerosols and droplets with sizes and velocities consistent with human breathing, talking, and coughing. The low-cost sensor platform will be deployed at various distances from an aerosolization source. Virus concentrations will be measured using the SERS assay and compared to results using RT-qPCR for validation. This effort will be the first to explicitly focus on development of low-cost SERS-based sensor platforms for detection of SARS-CoV2 within aerosols or droplets. Additional societal benefits will result from assessment of the validity of the “six foot” social-distancing recommendation for SARS-CoV2.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

COVID-19大流行在全球范围内影响了人类健康，迄今为止，死亡人数超过25万。越来越多的证据表明，Covid-19是通过在称为气溶胶的小呼吸液滴中存在SARS-COV2病毒传播的。为了减慢19009的传播，我们需要更好地了解这些液滴中的空气中流动多少。当前测量病毒的方法过于耗时，无法提供必要的信息，以使通过空气充分打击传播。使用纳米技术，拟议的研究将开发一种更快的方法来测量小滴中的病毒。这将使我们能够在更多位置测量病毒在室内空气中移动时跟踪它们。项目团队利用纳米技术，气溶胶科学和病毒检测的研究专业知识。该项目的成功完成将导致生产更快的Covid-19分析工具，该工具不需要RNA提取，可用于快速且廉价的测试。结果将用于评估建议社会距离建议的“六英尺规则”的科学有效性。迫切需要对呼吸道病毒SARS-COV2的快速和敏感的环境检测，该呼吸道病毒SARS-COV2是负责COVID-19的大流行的剂。 SARS-COV2检测的大多数当前方法都取决于RT-QPCR来定量病毒RNA。这些技术既是劳动力，又是成本密集的。这项研究的目的是开发一种快速的SARS-COV2检测方法，以测试SARS-COV2通过气溶胶传播的假设。第二个目标是评估推荐的六英尺社会距离指南是否在科学上有效，因为支持此建议的研究很少。这些目标将通过开发低成本传感器平台来实现，以快速检测使用表面增强的拉曼光谱法（SERS）在一个小时内检测病毒的SARS-COV2病毒。这项研究的次要目标是将开发的平台部署在建筑环境中，以量化SARS-COV2运输。该项目团队包括在纳米传感器开发，生物体特征和病毒检测方面具有免费专业知识的研究人员。在传感器平台的开发中，我们将使用塑料和织物优惠券以及金纳米纤维素和金粘性胶带SERS底物检查病毒的恢复和检测。将使用PHI6（SARS-COV2的替代物）评估建筑环境中雾化的SARS-COV2的运输，并将热灭活的SARS-COV2 NEBULED雾化，以产生与人类呼吸，通话和scouch的尺寸和速度的气溶胶和液滴。低成本传感器平台将在与气化源不同的距离内部署。病毒浓度将使用SERS分析测量，并与使用RT-QPCR进行验证的结果进行比较。这项工作将是第一个明确专注于开发基于低成本的传感器平台，以检测气溶胶或液滴中的SARS-COV2。评估SARS-COV的“六英尺”社会势不可及的建议的有效性。该奖项反映了NSF的法定任务，并通过基金会的智力优点和更广泛的审查标准通过评估来诚实地认为支持。

项目成果

Discriminatory Detection of ssDNA by Surface-Enhanced Raman Spectroscopy (SERS) and Tree-Based Support Vector Machine (Tr-SVM)

• DOI: 10.1021/acs.analchem.0c04576
• 发表时间: 2021-07-01
• 期刊: ANALYTICAL CHEMISTRY
• 影响因子: 7.4
• 作者: Kang, Seju;Kim, Inyoung;Vikesland, Peter J.
• 通讯作者: Vikesland, Peter J.

Environmental routes of virus transmission and the application of nanomaterial-based sensors for virus detection

• DOI: 10.1039/d2en00600f
• 发表时间: 2022-11-21
• 期刊: ENVIRONMENTAL SCIENCE-NANO
• 影响因子: 7.3
• 作者: Wang，Wei;Kang，Seju;Vikesland，Peter J.
• 通讯作者: Vikesland，Peter J.

Implications of the Coffee-Ring Effect on Virus Infectivity

• DOI: 10.1021/acs.langmuir.1c01610
• 发表时间: 2021-09-15
• 期刊: LANGMUIR
• 影响因子: 3.9
• 作者: Huang, Qishen;Wang, Wei;Vikesland, Peter J.
• 通讯作者: Vikesland, Peter J.

Recent advances in environmental science and engineering applications of cellulose nanocomposites

• DOI: 10.1080/10643389.2022.2082204
• 发表时间: 2022-06
• 期刊: Critical Reviews in Environmental Science and Technology
• 影响因子: 12.6
• 作者: A. Rahman;Wei Wang-;Divyapriya Govindaraj;Seju Kang;P. Vikesland

Surface-Enhanced Raman Spectroscopy of Bacterial Metabolites for Bacterial Growth Monitoring and Diagnosis of Viral Infection

细菌代谢物的表面增强拉曼光谱用于细菌生长监测和病毒感染诊断

• DOI: 10.1021/acs.est.1c02552
• 发表时间: 2021
• 期刊: Environmental Science & Technology
• 影响因子: 11.4
• 作者: Wang, Wei;Kang, Seju;Vikesland, Peter J.
• 通讯作者: Vikesland, Peter J.