CAREER: Transforming Multi-parametric Photoacoustic Microscopy

职业：改变多参数光声显微镜

• 批准号: 2023988
• 负责人: Song Hu
• 金额: $ 47.47万
• 依托单位: Washington University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-01-01 至 2025-05-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2023988&HistoricalAwards=false
• 关键词: CAREER; Transforming; Multi; parametric; Photoacoustic

This project aims to transform microscopy from the current low-speed benchtop system into the first-of-a-kind high-speed miniature/portable device that will enable wearable, handheld applications. The microscope will include many of the currently available separate optical modalities into a single multi-parameter system. The proposed system will retain these modalities with no reduction in performance yet with improved portability, reduction in size, and increased data collection efficiency for functional imaging capabilities. This breakthrough is expected to find broad applications in areas spanning from neuroscience to clinical treatment of cancer, personalized screening of cardiovascular drugs, and management of wound healing. The technology developed in this program will significantly expand the scope of multi-parametric PAM beyond basic research in anesthetized of restrained animals. The multidisciplinary nature of the proposed research involving optical and mechanical design, numerical simulation, microfabrication, data processing, instrument development and testing will offer well-balanced training opportunities for all participants.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该项目旨在将显微镜从当前的低速台式系统转变为首个高速微型/便携式设备，从而实现可穿戴、手持式应用。该显微镜将把许多目前可用的独立光学模式整合到一个多参数系统中。所提出的系统将保留这些模式，而不降低性能，同时提高便携性、减小尺寸并提高功能成像功能的数据收集效率。这一突破预计将在从神经科学到癌症临床治疗、心血管药物个性化筛选和伤口愈合管理等领域得到广泛应用。该项目开发的技术将显着扩展多参数 PAM 的范围，超越麻醉动物的基础研究。拟议研究的多学科性质涉及光学和机械设计、数值模拟、微加工、数据处理、仪器开发和测试，将为所有参与者提供均衡的培训机会。该奖项反映了 NSF 的法定使命，并被认为值得通过以下方式提供支持：使用基金会的智力价值和更广泛的影响审查标准进行评估。

项目成果

Development of a photoacoustic microscopy technique to assess peritubular capillary function and oxygen metabolism in the mouse kidney

开发光声显微镜技术评估小鼠肾脏管周毛细血管功能和氧代谢

• DOI: 10.1016/j.kint.2021.06.018
• 发表时间: 2021
• 期刊: Kidney International
• 影响因子: 19.6
• 作者: Sun, Naidi;Zheng, Shuqiu;Rosin, Diane L.;Poudel, Nabin;Yao, Junlan;Perry, Heather M.;Cao, Rui;Okusa, Mark D.;Hu, Song
• 通讯作者: Hu, Song

High-speed wide-field multi-parametric photoacoustic microscopy.

• DOI: 10.1364/ol.391824
• 发表时间: 2020-05-15
• 期刊: Optics letters
• 影响因子: 3.6
• 作者: Zhong F;Bao Y;Chen R;Zhou Q;Hu S
• 通讯作者: Hu S

Temporal and spectral unmixing of photoacoustic signals by deep learning.

• DOI: 10.1364/ol.426678
• 发表时间: 2021-06-01
• 期刊: OPTICS LETTERS
• 影响因子: 3.6
• 作者: Zhou, Yifeng;Zhong, Fenghe;Hu, Song
• 通讯作者: Hu, Song

Thin-film optical-acoustic combiner enables high-speed wide-field multi-parametric photoacoustic microscopy in reflection mode.

• DOI: 10.1364/ol.475373
• 发表时间: 2023-01
• 期刊: Optics letters
• 影响因子: 3.6
• 作者: Fenghe Zhong;Song Hu

Sparse Coding-Enabled Low-Fluence Multi-Parametric Photoacoustic Microscopy.

• DOI: 10.1109/tmi.2021.3124124
• 发表时间: 2022-04
• 期刊: IEEE transactions on medical imaging
• 影响因子: 10.6