Collaborative Research: Species delimitation in North American lizards

合作研究：北美蜥蜴的物种界定

• 批准号: 2023723
• 负责人: Adam Leache
• 金额: $ 42.15万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2023723&HistoricalAwards=false
• 关键词: Collaborative; Research; Species; delimitation; North

Species are a fundamental unit of diversity in the natural world. Therefore, accurately documenting species diversity has important implications in life sciences, habitat management, and conservation. Yet, despite the importance of species in understanding biodiversity, it is often difficult to identify unambiguous species boundaries in many taxonomic groups. Lizards are one such example of a taxonomic group with genetically-different populations that often look very similar. What appears to be one lizard species is often a species group of several possible lizard species. In this project, the researchers will collaborate with scientists across 15 different institutions to determine species boundaries in 30 species groups of North American lizards. Doing so will (1) connect scientific understandings of how species form to the practice of naming species and (2) model an intellectual and methodological framework for naming species consistently in other taxonomic groups. The researchers will share their research findings at community events across three metropolitan regions, engage undergraduate students in authentic research, and guide taxonomic revisions to promote stable species names.This project will use an integrative approach to delimit species. First, the project team will collaborate with taxonomic experts to generate range-wide genetic, ecological, and morphological sampling for each focal species group. These data will then be analyzed consistently to characterize levels of genetic and phenotypic divergence within each species complex. Then, the project team will assay levels of reproductive isolation between candidate species by using genetic data to measure the extent of hybridization and introgression between them. By comparing data on divergence and isolation, the team will characterize the rate and pace at which reproductive isolation evolves. Using these estimates, the team will then define the population-species boundary and apply it to the 30 focal groups to identify taxonomic units that show the highest levels of durability through time. Finally, using the revised taxonomy, the team will evaluate species delimitation methods that define taxonomic units based solely on genetic data. While efficient, these methods can be biased, and this analysis will identify the potential causes for these biases.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

物种是自然界多样性的基本单位。因此，准确记录物种多样性在生命科学，栖息地管理和保护中具有重要意义。然而，尽管物种在理解生物多样性中的重要性，但在许多分类群体中通常很难识别出明确的物种边界。蜥蜴是一个分类群体的一个例子，具有遗传学不同的人群，通常看起来非常相似。似乎是一种蜥蜴物种通常是几种可能的蜥蜴物种的种群。在该项目中，研究人员将与15个不同机构的科学家合作，以确定北美蜥蜴30种种类的物种边界。这样做将（1）连接对物种形成方式的科学理解与命名物种的实践，以及（2）对其他分类群中始终如一地命名物种的智力和方法框架。研究人员将在三个大都市地区的社区活动中分享他们的研究结果，使本科生参与真实研究，并指导分类学修订以促进稳定的物种名称。该项目将使用一种综合方法来界定物种。首先，该项目团队将与分类专家合作，为每个焦点物种群生成范围范围内的遗传，生态和形态采样。然后，将对这些数据进行一致分析，以表征每个物种复合物中遗传和表型差异的水平。然后，项目团队将通过使用遗传数据来测量候选物种之间的生殖分离水平来衡量它们之间的杂交程度和渗入程度。通过比较有关差异和隔离的数据，团队将表征生殖隔离的速度和节奏。然后，使用这些估计值，团队将定义人口种群边界，并将其应用于30个焦点组，以识别显示出随着时间的推移效果最高水平的分类单元。最后，使用修订后的分类法，该团队将评估仅根据遗传数据定义分类单元的物种划界方法。虽然有效，但这些方法可能会有偏见，并且该分析将确定这些偏见的潜在原因。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的智力优点和更广泛的影响审查标准通过评估来获得支持的。

项目成果

Phylogenomic Assessment of Biodiversity Using a Reference-Based Taxonomy: An Example With Horned Lizards (Phrynosoma)

• DOI: 10.3389/fevo.2021.678110
• 发表时间: 2021-07
• 作者: A. Leaché;Hayden R. Davis;S. Singhal;M. Fujita;Megan E. Lahti;K. Zamudio

A chromosome-level genome assembly for the eastern fence lizard (Sceloporus undulatus), a reptile model for physiological and evolutionary ecology.

• DOI: 10.1093/gigascience/giab066
• 发表时间: 2021-10-01
• 期刊: GigaScience
• 影响因子: 9.2
• 作者: Westfall AK;Telemeco RS;Grizante MB;Waits DS;Clark AD;Simpson DY;Klabacka RL;Sullivan AP;Perry GH;Sears MW;Cox CL;Cox RM;Gifford ME;John-Alder HB;Langkilde T;Angilletta MJ;Leaché AD;Tollis M;Kusumi K;Schwartz TS
• 通讯作者: Schwartz TS

Molecular Identification of Sceloporus Lizards in the Laramie Mountains, Wyoming

怀俄明州拉勒米山脉中的Sceloporus 蜥蜴的分子鉴定

• DOI: 10.3398/064.081.0412
• 发表时间: 2021
• 期刊: Western North American Naturalist
• 影响因子: 0.6
• 作者: Milcoff, Kiara W.;Estes-Zumpf, Wendy A.;Leaché, Adam D.
• 通讯作者: Leaché, Adam D.