Collaborative Research: Species delimitation in North American lizards

合作研究：北美蜥蜴的物种界定

• 批准号: 2023723
• 负责人: Adam Leache
• 金额: $ 42.15万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2023723&HistoricalAwards=false
• 关键词: Collaborative; Research; Species; delimitation; North

Species are a fundamental unit of diversity in the natural world. Therefore, accurately documenting species diversity has important implications in life sciences, habitat management, and conservation. Yet, despite the importance of species in understanding biodiversity, it is often difficult to identify unambiguous species boundaries in many taxonomic groups. Lizards are one such example of a taxonomic group with genetically-different populations that often look very similar. What appears to be one lizard species is often a species group of several possible lizard species. In this project, the researchers will collaborate with scientists across 15 different institutions to determine species boundaries in 30 species groups of North American lizards. Doing so will (1) connect scientific understandings of how species form to the practice of naming species and (2) model an intellectual and methodological framework for naming species consistently in other taxonomic groups. The researchers will share their research findings at community events across three metropolitan regions, engage undergraduate students in authentic research, and guide taxonomic revisions to promote stable species names.This project will use an integrative approach to delimit species. First, the project team will collaborate with taxonomic experts to generate range-wide genetic, ecological, and morphological sampling for each focal species group. These data will then be analyzed consistently to characterize levels of genetic and phenotypic divergence within each species complex. Then, the project team will assay levels of reproductive isolation between candidate species by using genetic data to measure the extent of hybridization and introgression between them. By comparing data on divergence and isolation, the team will characterize the rate and pace at which reproductive isolation evolves. Using these estimates, the team will then define the population-species boundary and apply it to the 30 focal groups to identify taxonomic units that show the highest levels of durability through time. Finally, using the revised taxonomy, the team will evaluate species delimitation methods that define taxonomic units based solely on genetic data. While efficient, these methods can be biased, and this analysis will identify the potential causes for these biases.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

物种是自然界多样性的基本单位。因此，准确记录物种多样性对生命科学、栖息地管理和保护具有重要意义。然而，尽管物种对于理解生物多样性很重要，但在许多分类群中通常很难确定明确的物种边界。蜥蜴就是这样一个分类群体的例子，其种群的基因不同，但通常看起来非常相似。看似一种蜥蜴物种通常是由几种可能的蜥蜴物种组成的物种组。在这个项目中，研究人员将与 15 个不同机构的科学家合作，确定 30 个北美蜥蜴物种组的物种边界。这样做将（1）将对物种形成方式的科学理解与物种命名实践联系起来，（2）为在其他分类群中一致命名物种建立一个知识和方法框架。研究人员将在三个大都市区的社区活动中分享他们的研究成果，让本科生参与真实的研究，并指导分类学修订以促进稳定的物种名称。该项目将使用综合方法来界定物种。首先，项目团队将与分类学专家合作，为每个焦点物种组生成广泛的遗传、生态和形态采样。然后将对这些数据进行一致分析，以表征每个物种复合体内的遗传和表型分化水平。然后，项目团队将通过使用遗传数据来测量候选物种之间的杂交和渗入程度，来测定候选物种之间的生殖隔离水平。通过比较分歧和隔离的数据，研究小组将描述生殖隔离发展的速度和速度。利用这些估计，该团队将定义种群-物种边界，并将其应用于 30 个焦点群体，以确定随着时间的推移显示出最高耐久性水平的分类单元。最后，使用修订后的分类法，该团队将评估仅基于遗传数据定义分类单位的物种界定方法。虽然这些方法有效，但可能存在偏差，并且该分析将确定这些偏差的潜在原因。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Phylogenomic Assessment of Biodiversity Using a Reference-Based Taxonomy: An Example With Horned Lizards (Phrynosoma)

• DOI: 10.3389/fevo.2021.678110
• 发表时间: 2021-07
• 作者: A. Leaché;Hayden R. Davis;S. Singhal;M. Fujita;Megan E. Lahti;K. Zamudio

A chromosome-level genome assembly for the eastern fence lizard (Sceloporus undulatus), a reptile model for physiological and evolutionary ecology.

• DOI: 10.1093/gigascience/giab066
• 发表时间: 2021-10-01
• 期刊: GigaScience
• 影响因子: 9.2
• 作者: Westfall AK;Telemeco RS;Grizante MB;Waits DS;Clark AD;Simpson DY;Klabacka RL;Sullivan AP;Perry GH;Sears MW;Cox CL;Cox RM;Gifford ME;John-Alder HB;Langkilde T;Angilletta MJ;Leaché AD;Tollis M;Kusumi K;Schwartz TS
• 通讯作者: Schwartz TS

Molecular Identification of Sceloporus Lizards in the Laramie Mountains, Wyoming

怀俄明州拉勒米山脉中的Sceloporus 蜥蜴的分子鉴定

• DOI: 10.3398/064.081.0412
• 发表时间: 2021
• 期刊: Western North American Naturalist
• 影响因子: 0.6
• 作者: Milcoff, Kiara W.;Estes-Zumpf, Wendy A.;Leaché, Adam D.
• 通讯作者: Leaché, Adam D.