DISSERTATION RESEARCH: Integrating Physiology and Genomics to Test for Local Adaptation in a Montane Ectotherm

论文研究：整合生理学和基因组学来测试山地变温动物的局部适应

• 批准号: 1701231
• 负责人: Adam Leache
• 金额: $ 2.01万
• 依托单位: University of Washington
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-06-15 至 2019-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1701231&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Integrating; Physiology; Genomics

This project will investigate the processes determining color and size differences between western fence lizards occurring at high versus low elevations on mountains in California. High elevation lizards are darker and larger than low elevation lizards. Such differences in traits within a species are generally assumed to be genetically based and a result of adaptation to local environments. However, phenotypic differences can also result from the effects of different environmental conditions on development. The researchers will use a combination of physiological experiments and genomic analyses to identify whether color and size differences in western fence lizards are a consequence of natural selection or phenotypic plasticity. Results will provide critical insights into the role and evolutionary advantages of environmentally dependent phenotypic differences in heterogeneous environments. In addition, the project includes day-long educational workshops targeting young girls interested in STEM fields and development of an interactive exhibit on lizards for a local museum.This study focuses on a phenotypic cline in the western fence lizard (Sceloporus occidentalis) along an elevational gradient in the Sierra Nevada Mountains of California. By combining techniques from genomics, functional ecology, and transcriptomics, this research will develop a comprehensive picture of the origin and function of high elevation phenotypes in western fence lizards. The researchers plan to evaluate the influence of temperature and day length on juvenile phenotypes, and to identify candidate genomic regions implicated in phenotypic differentiation. Juvenile lizards will be reared in a laboratory common garden experiment with treatments that mimic conditions at high and low elevation. This experimental design will decouple the genetic and environmental constituents of phenotype, and also shed light on the functional implications of trait differences. By comparing gene expression profiles of lizards reared in native and non-native conditions, fixed and plastic responses to environmental conditions at both organismal and genomic levels will be identified.

该项目将研究确定加州山区高海拔和低海拔西部栅栏蜥蜴颜色和体型差异的过程。高海拔蜥蜴比低海拔蜥蜴颜色更深、体型更大。通常认为物种内的这种性状差异是基于遗传的，并且是适应当地环境的结果。然而，不同的环境条件对发育的影响也可能导致表型差异。研究人员将结合生理实验和基因组分析来确定西部栅栏蜥蜴的颜色和大小差异是否是自然选择或表型可塑性的结果。结果将为异质环境中环境依赖性表型差异的作用和进化优势提供重要的见解。此外，该项目还包括针对对 STEM 领域感兴趣的年轻女孩举办为期一天的教育讲习班，并为当地博物馆开发蜥蜴互动展览。这项研究重点关注沿海拔的西部栅栏蜥蜴（Sceloporus occidentalis）的表型谱系。加利福尼亚州内华达山脉的梯度。通过结合基因组学、功能生态学和转录组学技术，这项研究将全面了解西部栅栏蜥蜴高海拔表型的起源和功能。研究人员计划评估温度和日照长度对幼年表型的影响，并确定与表型分化有关的候选基因组区域。幼年蜥蜴将在实验室普通花园中进行饲养，并进行模拟高海拔和低海拔条件的处理。该实验设计将解耦表型的遗传和环境成分，并揭示性状差异的功能含义。通过比较在本地和非本地条件下饲养的蜥蜴的基因表达谱，将确定生物体和基因组水平上对环境条件的固定和可塑反应。