Collaborative Research: Plant-based Pathogen Filters

合作研究：基于植物的病原体过滤器

基本信息

批准号：
2022971
负责人：
Manish Kumar
金额：
$ 18.5万
依托单位：
University of Texas at Austin
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2023-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=2022971&HistoricalAwards=false
关键词：
Collaborative Research Plant based Pathogen

项目摘要

Viral contamination of drinking water can cause disease. Although such waterborne diseases pose significant public health threats, current filtration technologies suitable for virus removal have high cost and energy requirements. This prevents their widespread use and has led to the need for less expensive and sustainable alternatives for disinfecting drinking water. The goal of this project is to use computational biology tools to discover plant-based peptides that can trap viruses to create low-cost and energy-efficient drinking water filters. The potential for scale up will be assessed to understand the impacts of common water constituents on virus-protein interactions to improve sustainable and effective filter operation. Creation of a large database of plant peptides will be broadly informative to other scientific disciplines and easily accessible via internet resource to be developed as part of this project. Successful development of plant-based water biofilters will have a range of potential applications including the replacement of aging infrastructure and use in disaster relief situations. More broadly, low cost filters have significant potential to decrease waterborne disease deaths worldwide through improved access to clean drinking water. Water contamination with human enteric viruses is one of the leading causes of acute diarrhea hospitalization and fatalities worldwide. Removal of these viruses can be achieved through filtration and other water treatment technologies. Although filtration has been one of the most widely used techniques for drinking water treatment, there has been comparatively little innovation in filtration in recent decades. The goal of this project is to develop sustainable, scalable filters functionalized with sustainable plant material known as antimicrobial peptides (AMPs). Specific objectives designed to achieve the project goal focus on understanding the fundamental biomolecular and physicochemical interactions to: 1) Computationally identify plant-derived AMPs that preferentially bind viral capsid proteins for use in plant-based pathogen filters; 2) Validate chosen AMPs using lab removal tests with the enteric viruses Adenovirus 2, Rotavirus OSU strain, and Tulane virus (a surrogate for human norovirus); 3) Identify readily available substrates and feasible filter configurations that can be tailored to a range of applications; and 4) Quantify interactions and interferences between filter surfaces, viruses, and water matrix components to enable translation to the field scale. Successful completion of these research objectives will lead to the development of a data-driven framework for creating and evaluating AMP-based materials. Further, low cost-effective plant-based pathogen filters have the potential to reduce waterborne disease worldwide through widespread adoption of the technology.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

饮用水的病毒污染会导致疾病。尽管这种水传播疾病构成了重大的公共卫生威胁，但当前适合去除病毒的过滤技术具有很高的成本和能源需求。这样可以防止他们广泛使用，并导致需要廉价且可持续的替代品来消毒饮用水。该项目的目的是使用计算生物学工具来发现基于植物的肽，这些肽可以捕获病毒以产生低成本和节能的饮用水过滤器。将评估扩大规模的潜力，以了解常见水成分对病毒蛋白质相互作用的影响，以改善可持续和有效的过滤器操作。创建大型植物肽数据库将为其他科学学科提供广泛的信息，并可以通过互联网资源易于访问，以作为该项目的一部分开发。成功开发基于植物的水产品将具有一系列潜在的应用，包括更换老化的基础设施以及在救灾情况下使用。更广泛地说，低成本过滤器具有通过改善清洁饮用水的机会来减少世界疾病死亡的巨大潜力。人类肠道病毒的水污染是全球急性腹泻住院和死亡的主要原因之一。可以通过过滤和其他水处理技术来清除这些病毒。尽管过滤一直是饮用水处理最广泛的技术之一，但近几十年来过滤的创新相对较少。该项目的目的是开发可持续的可扩展过滤器，可将可持续的植物材料功能化，称为抗菌肽（AMP）。旨在实现项目目标的特定目标集中在理解基本的生物分子和物理化学相互作用上：1）计算识别植物来源的AMP，这些AMP优先结合病毒capsid蛋白，用于植物性病原体过滤器； 2）使用肠道病毒2，轮状病毒OSU菌株和Tulane病毒（人类诺如病毒的替代物）使用肠病毒2，轮状病毒OSU菌株和肠道病毒的实验室去除测试验证选定的AMP； 3）确定可以根据一系列应用程序量身定制的易于可用的基材和可行的过滤器配置； 4）量化滤波器表面，病毒和水基质组件之间的相互作用和干扰，以使转换为现场尺度。这些研究目标的成功完成将导致开发以数据驱动的框架来创建和评估基于AMP的材料。此外，低是成本效益的植物病原体过滤器有可能通过广泛采用该技术来减少全球疾病。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响来通过评估来支持的。