CAS: Acoustically Driven, Voltage-Free Spray Interface to Couple Capillary Electrophoresis and Mass Spectrometry

CAS：声学驱动、无电压喷雾接口，用于耦合毛细管电泳和质谱分析

• 批准号: 2004021
• 负责人: Lisa Holland
• 金额: $ 38.41万
• 依托单位: West Virginia University Research Corporation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-15 至 2024-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=2004021&HistoricalAwards=false
• 关键词: CAS; Acoustically; Driven; Voltage; Free

With support from the Chemical Measurement and Imaging (CMI) Program in the Division of Chemistry, Professor Lisa Holland of West Virginia University is working to improve our ability to perform chemical analyses important for health studies, monitoring industrial processes, and many other elements of research and discovery. Specifically, a new instrumental interface is being developed to effectively couple a tool capable of separating the components of extremely low-volume solution samples (capillary electrophoresis, CE) with a very powerful means of identifying the separated components (mass spectrometry, MS). To meet this challenge, molecules in solution are converted to gas-phase ions by applying high frequency sound waves to the liquid exiting the capillary used for the separation. The approach significantly reduces solvent consumption relative to more widely used liquid chromatography/MS approaches, thereby improving sustainability. Students engaged in these innovative studies gain key interdisciplinary research skills. Dr. Holland is devising innovative ways to integrate concepts relevant to her research into her undergraduate courses, greatly enhancing student career preparation. A low-cost handheld instrument and accompanying curricular materials are being developed for use in the teaching laboratory and for continuing education training of scientists within and beyond the field of separations.The CE-MS interface being developed in the Holland lab focuses on acoustically driven nebulization, an approach by which the high separation efficiency of CE can be preserved while the ionization process remains unaffected by the electrophoretic current. Integrating the acoustic probe with the electrophoresis ground improves both the ruggedness and ease of use of the interface. Better understanding of the effect of acoustic energy on droplet formation is being pursued through collaboration with Professor Tony Jun Huang of Duke University, with an aim of improving the ionization efficiency. Parallel efforts seek to adapt electrokinetic sheath flow systems to enable MS interfaces for zero-flow separation approaches such as capillary isoelectric focusing and sieving. Dual detection with UV-visible absorbance and MS is also being developed.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

在化学系化学测量和成像 (CMI) 项目的支持下，西弗吉尼亚大学的 Lisa Holland 教授正在努力提高我们进行化学分析的能力，这对健康研究、监测工业过程和许多其他研究要素非常重要和发现。 具体来说，正在开发一种新的仪器接口，以有效地将能够分离极低体积溶液样品成分的工具（毛细管电泳，CE）与识别分离成分的非常强大的方法（质谱，MS）结合起来。 为了应对这一挑战，通过对离开用于分离的毛细管的液体施加高频声波，将溶液中的分子转化为气相离子。 相对于更广泛使用的液相色谱/质谱方法，该方法显着减少了溶剂消耗，从而提高了可持续性。 参与这些创新研究的学生获得关键的跨学科研究技能。霍兰德博士正在设计创新方法，将与她的研究相关的概念融入到她的本科课程中，从而极大地增强学生的职业准备。 正在开发一种低成本手持式仪器和随附的课程材料，用于教学实验室以及分离领域内外科学家的继续教育培训。荷兰实验室正在开发的 CE-MS 接口专注于声学驱动雾化，这种方法可以保持 CE 的高分离效率，同时电离过程不受电泳电流的影响。 将声学探头与电泳接地集成，提高了接口的耐用性和易用性。 通过与杜克大学的 Tony Jun Huang 教授合作，我们正在更好地了解声能对液滴形成的影响，以提高电离效率。 同时努力寻求采用电动鞘流系统，使 MS 接口能够用于零流量分离方法，例如毛细管等电聚焦和筛分。 紫外-可见光吸收和 MS 双重检测也在开发中。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。