MRI: Acquisition of an automated fluorescent microscope and imaging system for undergraduate biology and chemistry research and research training

MRI：采购自动荧光显微镜和成像系统，用于本科生物学和化学研究及研究培训

• 批准号: 1919847
• 负责人: Matthew Nelson
• 金额: $ 13.82万
• 依托单位: St Joseph's University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-09-01 至 2022-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1919847&HistoricalAwards=false
• 关键词: MRI; Acquisition; automated; fluorescent; microscope

An award is made to Saint Joseph's University (SJU) to purchase a wide-field automated fluorescent microscope and imaging system. This equipment enhances the research and research training within SJU's biology and chemistry departments. College students will be exposed to projects involving fluorescent microscopy in upper-level undergraduate and graduate coursework and as a part of their research experiences at SJU. Additionally, through SJU's established connections with the Philadelphia area's public education system, this instrument will allow for enhanced engagement with local elementary and high school students. Microscopy-based teaching projects will help foster an appreciation among these students for the natural sciences, teach students about research, and will inform their future career interests.Fluorescence microscopy is an essential component of the ongoing research of both the biology and chemistry departments at SJU. The new microscope and imaging system will be used as a key component in the following projects: (1) in vivo imaging of signaling molecules using fluorescence-based biosensors during sleep in nematodes; (2) immunofluorescence of brain slices to determine the impact of sleep and sleep deprivation on memory in mice; (3) visualization of light- and dark-adaptive cellular movements in the eyes of fish; and (4) direct observation of host-pathogen interactions using Ustilago maydis, a fungal pathogen of maize. Other projects using this equipment involve: (1) the maintenance of chromosome telomeres in baker's yeast; (2) oxygen sensing mechanism of the Heme-PAS protein FixL from nitrogen-fixing bacteria, S. meliloti; and (3) analysis of semiconductor nanocrystals or "quantum dots." Results of this research will be presented at local, national, and international conferences, and published in peer-reviewed journals.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

圣约瑟夫大学 (SJU) 获得了购买宽视场自动荧光显微镜和成像系统的奖励。该设备增强了 SJU 生物和化学系的研究和研究培训。大学生将在高年级本科生和研究生课程中接触到涉及荧光显微镜的项目，并将其作为他们在上海交通大学研究经历的一部分。此外，通过 SJU 与费城地区公共教育系统建立的联系，该工具将增强与当地中小学生的互动。 基于显微镜的教学项目将有助于培养这些学生对自然科学的认识，教授学生研究知识，并为他们未来的职业兴趣提供信息。荧光显微镜是上海交通大学生物系和化学系正在进行的研究的重要组成部分。新的显微镜和成像系统将作为以下项目的关键组成部分：（1）在线虫睡眠期间使用基于荧光的生物传感器对信号分子进行体内成像； （2）脑切片免疫荧光测定睡眠和睡眠剥夺对小鼠记忆力的影响； (3) 鱼眼中光适应和暗适应细胞运动的可视化； （4）利用玉米黑粉菌（一种玉米真菌病原体）直接观察宿主与病原体的相互作用。使用该设备的其他项目包括：（1）面包酵母中染色体端粒的维持； (2)固氮细菌苜蓿根瘤菌Heme-PAS蛋白FixL的氧传感机制； (3) 半导体纳米晶体或“量子点”的分析。 这项研究的结果将在地方、国家和国际会议上发表，并在同行评审的期刊上发表。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

An AMPK biosensor for Caenorhabditis elegans. microPublication Biology

用于秀丽隐杆线虫的 AMPK 生物传感器。

• DOI: 10.17912/micropub.biology.000596.
• 发表时间: 2022-07
• 期刊: microPublication biology
• 作者: Carman L;Schuck RJ;Li E;Nelson MD
• 通讯作者: Nelson MD

Orcokinin neuropeptides regulate sleep in Caenorhabditis elegans

Orcokinin 神经肽调节秀丽隐杆线虫的睡眠

• DOI: 10.1080/01677063.2020.1830084
• 发表时间: 2020-01
• 期刊: Journal of Neurogenetics
• 影响因子: 1.9
• 作者: Honer, Madison;Buscemi, Kristen;Barrett, Natalie;Riazati, Niknaz;Orlando, Gerald;Nelson, Matthew D.
• 通讯作者: Nelson, Matthew D.

The neuropeptide receptor npr-38 regulates avoidance and stress-induced sleep in Caenorhabditis elegans

神经肽受体 npr-38 调节秀丽隐杆线虫的回避和压力诱导的睡眠

• DOI: 10.1016/j.cub.2023.06.042
• 发表时间: 2023-07
• 期刊: Current Biology
• 影响因子: 9.2
• 作者: Le, Emily;McCarthy, Teagan;Honer, Madison;Curtin, Caroline E.;Fingerut, Jonathan;Nelson, Matthew D.
• 通讯作者: Nelson, Matthew D.