Evolution of a developmental gene regulatory network during a life history switch in Heliocidaris

Heliocidaris生命史转换过程中发育基因调控网络的进化

基本信息

批准号：
1929934
负责人：
Gregory Wray
金额：
$ 80万
依托单位：
Duke University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-01 至 2024-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1929934&HistoricalAwards=false
关键词：
Evolution developmental gene regulatory network

项目摘要

Multicellular organisms exhibit an astonishing diversity of life history strategies, including a wide range of lifespan, time to first reproduction, lifetime fecundity, and maternal investment. Life history traits often evolve independently of adult morphology and are a major influence on how organisms interact with each other and adapt to their environment. A particularly striking life history change in animals is accelerated embryonic development, which is favored under a variety of conditions including high predation and limited resources. A mature body of theory provides insights into why such changes in life histories evolve, but far less is known about how. The central objective of this project is to identify the genetic and molecular basis for changes in specific traits during a substantive shift in life history. The study system is a group of sea urchin species that are very closely related yet highly divergent in terms of life history traits, including striking differences in fecundity, larval form, and rate of early development. This project uses cutting-edge technologies, including epigenetic assays, single-cell sequencing, and genome editing to identify changes in gene function that contributed to a suite of life history traits. These studies will provide basic insights into how life histories and developmental mechanisms evolve. Understanding how alterations in molecular mechanisms contribute to traits and to adaptation is fundamental science that has important applications in medicine, agriculture, and biotechnology.Life history traits are a major component of biological diversity and constitute a fundamental set of adaptations in multicellular organisms, but their developmental and genetic basis remains poorly understood, particularly in relation to morphology and physiology. This project seeks to identify changes in the genome and in developmental mechanisms that produced a dramatic life history transformation within the sea urchin genus Heliocidaris, namely from planktotrophy (small eggs, high fecundity, feeding larvae) and lecithotrophy (large eggs, low fecundity, nonfeeding larvae). This genus is well suited to addressing the motivating questions because it contains species with dramatically divergent life histories despite very close phylogenetic relationships, and because the ancestral developmental gene regulatory network (GRN) of sea urchins has been characterized in detail. The goals of the project are to learn how critical molecular mechanisms of early development were altered during the course of evolution so as to accelerate premetamorphic the lecithotroph, and how they did so without perturbing other traits. These goals will be achieved by comprehensively characterizing regulatory states during development to identify candidate regulatory molecules and interactions that have been conserved or altered during the origin of lecithotrophy; experimentally perturbing candidate proteins (nodes in the GRN) to learn about their developmental function, likely interactors, and contributions to the origin of lecithotrophy; and identifying and perturbing candidate gene regulatory elements that may mediate altered interactions (edges in the GRN) to understand their molecular function and contributions to the origin of lecithotrophy.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

多细胞生物具有惊人的生活历史策略多样性，包括广泛的寿命，首次繁殖时间，终身繁殖力和产妇投资。生活史特征通常独立于成人形态而发展，这是对生物如何相互作用并适应其环境的主要影响。动物的生活历史的特别惊人的是胚胎发育加速，在各种疾病中，它受到青睐，包括高捕食和有限的资源。一个成熟的理论体验提供了有关生活历史上这样的变化为什么发展的见解，但对如何发展的知识却少得多。该项目的核心目的是确定在生活史发生实质性转变期间特定特征变化的遗传和分子基础。研究系统是一组海胆物种，在人生历史特征方面，它们密切相关但高度分歧，包括繁殖力，幼虫形式和早期发展速度的差异。该项目使用尖端技术，包括表观遗传测定，单细胞测序和基因组编辑来识别有助于一系列生活历史特征的基因功能的变化。这些研究将提供有关生活历史和发展机制如何发展的基本见解。了解分子机制的变化如何有助于特征和适应性的是基础科学，在医学，农业和生物技术学中具有重要的应用。生活历史特征是生物多样性的主要组成部分，并且构成了多细胞生物的基本适应性集合，但在多细胞生物中仍然是贫穷的理解，尤其是在较差的基础上，尤其是相关的摩擦。该项目旨在确定基因组和发育机制的变化，这些机制在海胆属Heliocidaris内产生了巨大的生活历史转变，即来自浮游物营养（小鸡蛋，高粪便，高粪便，喂养幼虫）和肠thotroghothrophrophrophrophophrophy（大鸡蛋，大鸡蛋，低粪便，低粪便，无饲料）。该属非常适合解决激励的问题，因为尽管有非常紧密的系统发育关系，但它包含具有巨大不同生活历史的物种，并且已经详细介绍了海胆的祖先发育基因调节网络（GRN）。该项目的目标是学习在进化过程中如何改变早期发展的关键分子机制，以加速前型卵巢菌，以及他们如何在不扰乱其他特征的情况下这样做。这些目标将通过全面表征开发过程中的调节状态来确定候选的调节分子以及在植物营养起源期间保守或改变的候选调节分子；实验性地扰动候选蛋白（GRN中的节点），以了解其发育功能，可能的相互作用者以及对卵巢营养起源的贡献；并识别和扰动候选基因调节元素，这些元素可能介导了改变的相互作用（GRN中的边缘），以了解其分子功能和对卵巢营养起源的贡献。这项奖项反映了NSF的法定任务，并被认为是通过基金会的智力优点和广泛影响的评估来评估CRETERIA的评估。

项目成果

期刊论文数量（14）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Near-Chromosomal-Level Genome Assembly of the Sea Urchin Echinometra lucunter , a Model for Speciation in the Sea

海胆 Echinometra lucunter 的近染色体水平基因组组装，海洋物种形成模型

DOI：
10.1093/gbe/evad093
发表时间：
2023
期刊：
Genome Biology and Evolution
影响因子：
3.3
作者：
Davidson, Phillip L;Lessios, Harilaos A;Wray, Gregory A;McMillan, W Owen;Prada, Carlos
通讯作者：
Prada, Carlos

Recent Reconfiguration Of An Ancient Developmental Gene Regulatory Network In Heliocidaris Sea Urchins

DOI：
10.1101/2022.03.03.482868
发表时间：
2022-08-05
期刊：
bioRxiv
影响因子：
0
作者：
Davidson, P. L.;Guo, H.;Wray, G. A.
通讯作者：
Wray, G. A.

Developmental single-cell transcriptomics in the Lytechinus variegatus sea urchin embryo

Lytechinus variegatus 海胆胚胎的发育单细胞转录组学

DOI：
10.1242/dev.198614
发表时间：
2021
期刊：
Development
影响因子：
4.6
作者：
Massri, Abdull J.;Greenstreet, Laura;Afanassiev, Anton;Berrio, Alejandro;Wray, Gregory A.;Schiebinger, Geoffrey;McClay, David R.
通讯作者：
McClay, David R.

Ocean acidification induces distinct transcriptomic responses across life history stages of the sea urchin Heliocidaris erythrogramma