DISSERTATION RESEARCH: Contribution of Mutations within Cis-Regulatory DNA to Molecular Phenotype and Organismal Fitness

论文研究：顺式调控 DNA 突变对分子表型和有机体适应性的贡献

• 批准号: 0508589
• 负责人: Gregory Wray
• 金额: $ 1.2万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-15 至 2007-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0508589&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Contribution; Mutations; within

Regulatory sequences control timing, location and level of gene expression, and are predicted to play a key role in molecular and organismal evolution. Despite the importance of this regulation and the abundance of variation in gene expression, the effect this variation has on the organism is not known. We propose to explore how induced mutations within specific regulatory regions in brewer's yeast affect the expression of neighboring genes, and to what extent these differences influences organismal fitness. This type of information has not been acquired for any organism. The scientific importance of this project is to provide a greater understanding of how variation within regulatory regions translates to gene expression and organismal fitness. There are two significant broader impacts of this work. First, high school students are involved with laboratory components of this project through the Howard Hughes Summer Program. Second, natural isolates have been collected from both local and Australian vineyards. This work is collaborative in that isolated strains are made available to their vineyard of origin, for future use in fermentation, and the agriculture community is exposed to the benefits of scientific research.

调节序列控制基因表达的时间，位置和水平，并预测在分子和有机体进化中起关键作用。 尽管这种调节的重要性和基因表达的差异很大，但这种变异对生物体的影响尚不清楚。 我们建议探索酿酒酵母中特定调节区域内诱导的突变如何影响邻近基因的表达，以及这些差异在多大程度上影响生物适应性。 这种类型的信息尚未用于任何生物体。该项目的科学重要性是对监管区域内的变异如何转化为基因表达和有机体适应性。 这项工作有两个重大的广泛影响。 首先，高中生通过霍华德·休斯（Howard Hughes）夏季计划参与该项目的实验室组件。其次，自然分离株是从当地和澳大利亚葡萄园收集的。 这项工作是合作的，因为孤立的菌株可用于其原籍葡萄园，供将来在发酵中使用，并且农业界受到科学研究的好处。