DISSERTATION RESEARCH: Contribution of Mutations within Cis-Regulatory DNA to Molecular Phenotype and Organismal Fitness

论文研究：顺式调控 DNA 突变对分子表型和有机体适应性的贡献

• 批准号: 0508589
• 负责人: Gregory Wray
• 金额: $ 1.2万
• 依托单位: Duke University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-15 至 2007-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0508589&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Contribution; Mutations; within

Regulatory sequences control timing, location and level of gene expression, and are predicted to play a key role in molecular and organismal evolution. Despite the importance of this regulation and the abundance of variation in gene expression, the effect this variation has on the organism is not known. We propose to explore how induced mutations within specific regulatory regions in brewer's yeast affect the expression of neighboring genes, and to what extent these differences influences organismal fitness. This type of information has not been acquired for any organism. The scientific importance of this project is to provide a greater understanding of how variation within regulatory regions translates to gene expression and organismal fitness. There are two significant broader impacts of this work. First, high school students are involved with laboratory components of this project through the Howard Hughes Summer Program. Second, natural isolates have been collected from both local and Australian vineyards. This work is collaborative in that isolated strains are made available to their vineyard of origin, for future use in fermentation, and the agriculture community is exposed to the benefits of scientific research.

调控序列控制基因表达的时间、位置和水平，预计在分子和有机体进化中发挥关键作用。 尽管这种调节很重要并且基因表达中存在大量变异，但这种变异对生物体的影响尚不清楚。 我们建议探索啤酒酵母特定调控区域内的诱导突变如何影响邻近基因的表达，以及这些差异在多大程度上影响有机体适应性。 尚未获得任何生物体的此类信息。该项目的科学重要性在于更好地了解调控区域内的变异如何转化为基因表达和有机体适应性。 这项工作有两个更广泛的重要影响。 首先，高中生通过霍华德休斯暑期项目参与该项目的实验室部分。其次，从当地和澳大利亚的葡萄园中收集了天然分离物。 这项工作是协作性的，因为将分离的菌株提供给其原产地葡萄园，以供将来用于发酵，并且农业界也能受益于科学研究。