Collaborative Research: RUI: Combined spatial and temporal analyses of population connectivity during a northern range expansion

合作研究：RUI：北部范围扩张期间人口连通性的时空综合分析

• 批准号: 1924604
• 负责人: Robert Toonen
• 金额: $ 27.35万
• 依托单位: University of Hawaii
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1924604&HistoricalAwards=false
• 关键词: Collaborative; Research; RUI; Combined; spatial

Where do young marine fish and shellfish come from? This project aims to improve our understanding of how coastal marine populations are connected in space and time. Coastal populations are replenished through the arrival of minuscule larvae that have been dispersed for weeks to months in the open ocean after spawning at remote sites. The combination of the long dispersal period of marine fish and shellfish larvae and the varying ocean currents results in complex patterns of "connectivity" among populations near and far. Identifying these patterns of connectivity is fundamental to marine science and critical for effective fisheries management and conservation, yet it remains an unresolved component of marine ecology. The study species is currently expanding its biogeographic range up the U.S. west coast. By genetically analyzing individuals from across the species' range, including offspring spawned in the laboratory by experimentally-crossed individuals collected in the field from throughout the species historical and expanded range, certain genes can serve to differentiate populations along the coast. The team leverages the statistical power of these geographically-informative genes to assign thousands of young collected in the field to the source populations that spawned them (across the species' range and over multiple years). The team then quantifies patterns of connectivity over multiple years, and tests fundamental hypotheses on the spatial scale, temporal variability, biogeographic patterns, and biophysical drivers of population connectivity. The project trains approximately two dozen U.S. university students in molecular ecology and marine science, as well as creating intellectual linkages among Ph.D.-granting and non-Ph.D.-granting universities. The project also supports further development of a K-12 education program that uses SCUBA diving and videography to teach elementary school students Next Generation Science Standards and train them for careers in science, technology, engineering and mathematics. Using a kelp forest gastropod and fisheries species (Kellet's whelk, Kelletia kelletii), this project combines genome-wide Restriction site Associated DNA (RAD) loci with transcriptomic loci identified from common-garden laboratory crosses of individuals from the species' historical and expanded range to identify geographically-informative loci that maximize power for individual assignment testing. Leveraging the combined power of these loci, genetic assignment of approximately three thousand recruit samples to 20 putative source populations allows the team to construct three independent years of connectivity matrices and test some of the most fundamental questions in marine ecology, including: 1) Are marine populations open or closed and at what scales? 2) To what degree is the evolutionary pattern of gene flow represented by single versus multiple generations of connectivity events? And, 3) How spatially heterogeneous and temporally variable is population connectivity? Can one year of connectivity data predict anything about the next? Additionally, by focusing on a range-expanding species with common life history traits, the team addresses a number of questions with broad applicability and significant ecological and societal implications: 4) How much is population connectivity influenced by post-recruitment demographic and evolutionary processes? 5) How well-connected are historic- and expanded-range populations? And, of particular relevance to climate change, 6) Are El Nino oceanographic conditions, which are predicted to increase in frequency and intensity this century, driving the poleward range expansion of this coastal marine species? By coupling common-garden experimental crosses to identify maximally-informative transcriptomic loci with genomic RAD analysis of field samples, this project aims to accurately and precisely quantify marine population connectivity in high gene flow species with large population sizes.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

年轻的海鱼和贝类从哪里来？该项目旨在提高我们对沿海海洋种群如何在时空之间建立联系的理解。沿海人口通过微小的幼虫的到来补充，这些幼虫在偏远地点产卵后在开阔的海洋中分散了数周至数月。海洋鱼类和贝类幼虫的长时间分散时期与洋流变化的结合导致了近距离人群之间“连通性”的复杂模式。确定这些连通性的模式对于海洋科学是基础，对于有效的渔业管理和保护至关重要，但它仍然是海洋生态学的尚未解决的组成部分。该研究物种目前正在扩大美国西海岸的生物地理范围。通过对整个物种范围内的个体进行遗传分析，包括通过实验性分解的个体在实验室中产生的后代，这些个体与整个物种的历史和扩展范围内收集的实验性分解的个体可以使某些基因有助于区分沿海地区的种群。该团队利用这些地理知名基因的统计能力将在现场收集的成千上万的年轻人分配给产生它们的来源人群（整个物种的范围以及多年来）。然后，团队量化了多年的连接模式，并在空间量表，时间变异性，生物地理模式和人口连通性的生物物理驱动因素上测试基本假设。该项目培训了大约二十名分子生态学和海洋科学的大学学生，并在博士学位和非PH.D.授予大学之间建立了智力联系。该项目还支持进一步开发K-12教育计划，该计划使用水肺潜水和摄影来教授小学生下一代科学标准，并培训他们从事科学，技术，工程和数学的职业。该项目使用海带森林胃足类和渔业物种（Kellet's Whelk，Kelletia kelletii），将全基因组的限制性位点（RAD）基因座与从该物种的历史范围内的个人群中确定的共同实验室crossi鉴定的转录基因座相结合，以识别分配范围的范围，以识别分配范围，从而确定了分配范围。利用这些基因座的综合力量，将大约三千名招募样本的遗传分配到20个推定的源群体，使团队能够构建三个独立的连接矩阵，并测试海洋生态学中一些最基本的问题，包括：1）是海洋人群开放或关闭的，以及在什么规模上？ 2）基因流的进化模式由单个连接事件与多代连接事件表示？ 3）在空间上有异质和时间上的变化是种群连通性的？连通性数据一年可以预测下一个有关吗？此外，通过关注具有共同生活历史特征的范围扩展物种，该团队解决了许多具有广泛适用性以及重要的生态和社会意义的问题：4）受到后培训后人口统计学和进化过程影响多少人口连通性？ 5）连接的历史和扩大范围的人口如何？并且，与气候变化特别相关的是，6）是否有El Nino海洋学条件，预计本世纪的频率和强度会增加，推动了这种沿海海洋物种的极端范围扩展？通过将共同花园实验杂交耦合，以与现场样本的基因组RAD分析确定最大信息的转录基因座，该项目旨在准确，精确地量化具有较大人口大小的高基因流量物种中的海洋种群连通性。该奖项奖励NSF的法定任务，并通过评估范围来弥补基金会的支持，并反映了对基础的支持和基础的支持。

项目成果

Genomic divergence and differential gene expression between crustacean ecotypes across a marine thermal gradient

海洋热梯度甲壳动物生态型之间的基因组分歧和差异基因表达

• DOI: 10.1016/j.margen.2021.100847
• 发表时间: 2021
• 期刊: Marine Genomics
• 影响因子: 1.9
• 作者: Emami-Khoyi, Arsalan;Knapp, Ingrid S.;Monsanto, Daniela M.;van Vuuren, Bettine Jansen;Toonen, Robert J.;Teske, Peter R.
• 通讯作者: Teske, Peter R.

Interdisciplinary analysis of larval dispersal for a coral reef fish: opening the black box

珊瑚礁鱼幼虫扩散的跨学科分析：打开黑匣子

• DOI: 10.3354/meps13971
• 发表时间: 2022
• 期刊: Marine Ecology Progress Series
• 影响因子: 2.5
• 作者: Counsell, CWW;Coleman, RR;Lal, SS;Bowen, BW;Franklin, EC;Neuheimer, AB;Powell, BS;Toonen, RJ;Donahue, MJ;Hixon, MA
• 通讯作者: Hixon, MA

Fish Flow: following fisheries from spawning to supper

鱼流：跟踪从产卵到晚餐的渔业过程

• DOI: 10.1002/fee.2449
• 发表时间: 2022
• 期刊: Frontiers in Ecology and the Environment
• 影响因子: 10.3
• 作者: Hixon, Mark A;Bowen, Brian W;Coleman, Richard R;Counsell, Chelsie W;Donahue, Megan J;Franklin, Erik C;Kittinger, John N;McManus, Margaret A;Toonen, Robert J
• 通讯作者: Toonen, Robert J