MRI: Acquisition of a Matrix-Assisted Laser Desorption/Ionization Time-of-Flight(MALDI TOF) Mass Spectrometer for of Research and Research Training at CSULA

MRI：购买基质辅助激光解吸/电离飞行时间 (MALDI TOF) 质谱仪，用于 CSULA 的研究和研究培训

• 批准号: 1919722
• 负责人: Yong Ba
• 金额: $ 16.46万
• 依托单位: California State L A University Auxiliary Services Inc.
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-07-01 至 2022-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1919722&HistoricalAwards=false
• 关键词: MRI; Acquisition; Matrix; Assisted; Laser

This award is supported by the Major Research Instrumentation Program. Professor Yong Ba from California State University Los Angeles and colleagues Jamil Momand, Edith Porter, Yixian Wang and Cecilia Zurita-Lopez are acquiring a matrix-assisted laser desorption / ionization time-of-flight mass spectrometer (MALDI-TOF-MS) equipped with a photodiode array. In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex samples. MALDI TOF combines gentle ionization (ideal for producing intact ions of peptides, proteins, nucleic acids, carbohydrates, synthetic polymers, and other similarly sized species) with a detection mode that offers an excellent balance between sensitivity and accuracy across a wide range of samples. The acquisition strengthens the research infrastructure at the university and within the regional. The instrument broadens participation by giving hands-on access to a diverse student population. The instrument is also used in outreach activities.The award is aimed at enhancing research and education at all levels. It especially impacts studies correlating molecular structure, orientation and dynamics. Researchers use the MALDI-TOF-MS to study interactions of a mucoadhesive co-polymer colloidal gel systems and in identifying in vivo substrates and binding partners for the protein arginine methyltransferase 7 (PRMT7) in mammalian cells. The mass spectrometer is used for studying cell disruption mechanisms that may be operational in Parkinson's disease and for studying antifreeze proteins. The instrument also serves researchers identifying proteins associated with t-Darpp AKT activation proteins, in which t-Darpp is a truncated isoform of dopamine-regulated phosphoprotein having an elongated monomer that binds calcium and is phosphorylated by cyclin-dependent enzyme kinases. Finally, the instrument is used by researchers investigating antimicrobial peptides. Overall, the instrument acquisition may benefit the National health and welfare as well as supporting education and diversity.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项得到了主要的研究工具计划的支持。加利福尼亚州立大学洛杉矶和同事Jamil Momand，Edith Porter，Yixian Wang和Cecilia Zurita-Zurita-Lopez教授正在收购矩阵辅助的激光解吸 /电离时间飞行时间飞行时间量光谱质谱仪（Maldi-Tof-ms），配备了Photododiode Array。通常，质谱法（MS）是用于识别和表征复杂样品中少量化学物种的关键分析方法之一。 Maldi Tof结合了温和的电离（非常适合生产肽，蛋白质，核酸，碳水化合物，合成聚合物和其他类似尺寸的物种的完整离子和检测模式，该模式在广泛的样品范围内提供了良好的平衡。此次收购加强了大学和区域内的研究基础设施。该乐器通过动手访问多样化的学生群体来扩大参与。该仪器还用于外展活动。该奖项旨在增强各级研究和教育。它特别影响了将分子结构，方向和动力学相关的研究。 研究人员使用MALDI-TOF-MS研究粘附性共聚物胶体凝胶系统的相互作用，并在哺乳动物细胞中鉴定蛋白精氨酸甲基转移酶7（PRMT7）的体内底物和结合伴侣。质谱仪用于研究可能在帕金森氏病和研究抗冻蛋白中运作的细胞破坏机制。该仪器还为识别与T-DARPP AKT活化蛋白相关的蛋白质的研究人员提供服务，其中T-DARPP是具有延长钙的伸长单体并由CyClyly依赖Cyclin依赖性酶激酶磷酸化的多巴胺调节的磷酸蛋白的截断同工型。最后，研究人员研究了该仪器的抗菌肽。 总体而言，该工具的获取可能会受益于国家卫生和福利，并支持教育和多样性。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响评估标准通过评估来支持的。