Ethylene Cross-Kingdom Signaling In Beneficial Plant-Microbe Associations

有益植物-微生物关联中的乙烯跨界信号传导

基本信息

批准号：
1855066
负责人：
Brad Binder
金额：
$ 68.32万
依托单位：
University of Tennessee Knoxville
依托单位国家：
美国
项目类别：
Continuing Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-08-01 至 2024-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1855066&HistoricalAwards=false
关键词：
Ethylene Cross Kingdom Signaling Beneficial

项目摘要

With an increasing human population, future food security largely depends on sustainable agricultural practices that ensure elevated crop productivity with minimal environmental impact. Optimizing the microbial community that associates with plants is a possible strategy that is being pursued. Ethylene gas is produced by plants and functions as a plant hormone where it is known to affect many processes. Preliminary findings demonstrate that ethylene receptors are present in bacteria as well as plants. Many of these bacteria associate with plants and with none being pathogenic, it follows that ethylene produced by plants may function to regulate beneficial plant-microbe interactions. Results from this research on the role of ethylene in plant-microbe interactions will provide information needed to improve associations between plants and beneficial microbes to enhance food production and security. The research will also increase educational infrastructure by broadening opportunities for undergraduate and graduate students at the UT-Knoxville to engage in modern biological research. The project will test the hypothesis that ethylene produced by plants mediates the establishment of some beneficial bacteria in the rhizosphere via regulating biofilm formation and attachment of the bacteria to the plants. Preliminary data demonstrate that the genomes of several non-pathogenic and non-symbiotic, but potentially beneficial, proteobacteria encode homologs to plant ethylene receptors, with the Azospirillum brasilense homolog binding ethylene in vitro. Further evidence suggests that ethylene sensing by the bacteria modulates biofilm formation. The molecular mechanism of ethylene signaling and associated responses in bacteria will be characterized using a combination of genetic and biochemical approaches in genetically amenable bacterial models. The role of ethylene signaling in modulating root surface colonization by beneficial bacteria will be studied using plant colonization assays combined with genetic or chemical manipulation of ethylene signaling in model and crop plants. The possibility that ethylene signaling between plant and beneficial bacteria functions to modulate plant immune responses will also be tested. Results from the proposed research will generate novel models for plant-bacteria signaling that could yield innovative strategies to engineer beneficial plant-microbe associations in the rhizosphere.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

随着人口的不断增加，未来的粮食安全在很大程度上取决于可持续的农业实践，以确保提高作物生产力，同时将环境影响降至最低。优化与植物相关的微生物群落是一种可能的策略。乙烯气体由植物产生，作为植物激素发挥作用，已知它会影响许多过程。初步研究结果表明，细菌和植物中都存在乙烯受体。许多这些细菌与植物相关，但没有一种细菌是致病性的，因此植物产生的乙烯可能起到调节有益的植物-微生物相互作用的作用。这项关于乙烯在植物-微生物相互作用中的作用的研究结果将提供改善植物与有益微生物之间的联系所需的信息，从而提高粮食生产和安全。该研究还将通过扩大德州大学诺克斯维尔分校本科生和研究生从事现代生物学研究的机会来加强教育基础设施。该项目将测试以下假设：植物产生的乙烯通过调节生物膜的形成和细菌对植物的附着来介导一些有益细菌在根际的建立。初步数据表明，几种非致病性和非共生但可能有益的变形菌的基因组编码植物乙烯受体的同源物，巴西固氮螺菌同源物在体外结合乙烯。进一步的证据表明细菌对乙烯的感应调节生物膜的形成。将在适合遗传的细菌模型中结合遗传和生化方法来表征细菌中乙烯信号传导和相关反应的分子机制。将使用植物定植测定结合模型和作物植物中乙烯信号传导的遗传或化学操作来研究乙烯信号传导在调节有益细菌的根表面定殖中的作用。植物和有益细菌之间的乙烯信号传导调节植物免疫反应的可能性也将得到测试。拟议研究的结果将产生植物-细菌信号传导的新模型，从而产生创新策略，在根际设计有益的植物-微生物关联。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力价值进行评估，被认为值得支持以及更广泛的影响审查标准。

项目成果

期刊论文数量（3）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Multiple CheY Proteins Control Surface-Associated Lifestyles of Azospirillum brasilense

多种 CheY 蛋白控制巴西固氮螺菌的表面相关生活方式

DOI：
10.3389/fmicb.2021.664826
发表时间：
2021
期刊：
Frontiers in Microbiology
影响因子：
5.2
作者：
Ganusova EE;Vo LT;Mukherjee T;Alexandre G
通讯作者：
Alexandre G

Cytokinin and Ethylene Cell Signaling Pathways from Prokaryotes to Eukaryotes

从原核生物到真核生物的细胞分裂素和乙烯细胞信号传导途径

DOI：
10.3390/cells9112526
发表时间：
2020-11-23
期刊：
Cells
影响因子：
6
作者：
Bidon B;Kabbara S;Courdavault V;Glévarec G;Oudin A;Héricourt F;Carpin S;Spíchal L;Binder BM;Cock JM;Papon N
通讯作者：
Papon N

Bacterial Homologs of Progestin and AdipoQ Receptors (PAQRs) Affect Membrane Energetics Homeostasis but Not Fluidity

孕激素和 AdipoQ 受体 (PAQR) 的细菌同系物影响膜能量稳态但不影响流动性

DOI：
10.1128/jb.00583-21
发表时间：
2022-04-19
期刊：
Journal of Bacteriology
影响因子：
3.2
作者：
Melchionna, Maddison V.;Gullett, Jessica M.;Bouveret, Emmanuelle;Shrestha, Him K.;Abraham, Paul E.;Hettich, Robert L.;Alexandre, Gladys
通讯作者：
Alexandre, Gladys