Understanding Molecular-Recognition Properties of Helical Pores under Non-equilibrium Conditions

了解非平衡条件下螺旋孔的分子识别特性

• 批准号: 1905094
• 负责人: Bing Gong
• 金额: $ 22万
• 依托单位: SUNY at Buffalo
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2022-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1905094&HistoricalAwards=false
• 关键词: Understanding; Molecular; Recognition; Properties; Helical

Professor Bing Gong of the State University of New York at Buffalo and Professor Xiao Cheng Zeng of the University of Nebraska-Lincoln collaborate to develop novel approaches to sensing differences among sugar molecules. In this project, the team designs and synthesizes long-chain molecules that coil up like springs. The interior space of these "molecular springs" interact with different kinds of sugar molecules with different strengths. By embedding these "molecular springs" in a cell wall and monitoring the passage of sugar molecules through the interior spaces, the research team aims to develop a method to recognize and sense sugars in water. The results of this research may have implications in separations and medical diagnostics. The broader impacts involve training graduate and undergraduate students, incorporating research into teaching, conducting workshops, reaching out to underrepresented groups, and broadly disseminating this work to the scientific community. With the support from the Macromolecular, Supramolecular and Nanochemistry Program of the Chemistry Division, this project aims to probe and understand molecular recognition of sugar and sugar alcohols in aqueous media under non-equilibrium conditions. The research team prepares aromatic oligoamide foldamers that form helical coils. When embedded in membranes, the oxygen-rich, electrostatically negative inner pores of these coils provide thruways for ions to pass through the membrane. In addition, the presence of numerous amide oxygen atoms in the inner pores of the membrane-bound foldamers serve as hydrogen-bond acceptors that enable multiple hydrogen-bonding interactions with sugars and sugar alcohols in different strengths. Such interactions, being dynamic and transient, especially in water, result in the perturbation of ion currents through the transmembrane pores. Depending on their structures and properties, different types of carbohydrates perturb the flow of ions through the pores differently, which can be detected very sensitively. By measuring the changes in transmembrane ion conductance in the presence of different saccharides and their derivatives, the research aims to monitor and understand molecular recognition of these molecules under non-equilibrium conditions. Specifically, this work focuses on synthesizing oligoamides of different lengths (8mer, 16mer, 32mer, and 64mer) and examining the effects of various sugars and sugar alcohols on the transmembrane ion currents through the pore-forming foldamers. Vesicle-based assays are performed to provide a rapid but qualitative screening of the effects of chosen carbohydrates, followed by measurement of single-channel currents to reveal the signatures of different carbohydrate molecules.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

纽约州立大学布法罗州立大学的Bing Gong教授和内布拉斯加州林肯大学的肖Zeng教授合作开发了新颖的方法来感知糖分子之间的差异。 在这个项目中，团队设计和合成了像弹簧一样凝结的长链分子。 这些“分子弹簧”的内部空间与不同强度的不同种类的糖分子相互作用。 通过将这些“分子弹簧”嵌入细胞壁中，并监测糖分子通过内部空间的通过，研究小组的目标是开发一种方法来识别和感知水中的糖。 这项研究的结果可能对分离和医学诊断有影响。 更广泛的影响涉及培训毕业生和本科生，将研究纳入教学，进行讲习班，与代表性不足的群体接触，并将这项工作大致传播给科学界。在化学部的大分子，超分子和纳米化学计划的支持下，该项目旨在在非平衡条件下探测和了解水性培养基中糖和糖醇的分子识别。 研究小组准备形成螺旋线圈的芳族寡酰胺折叠剂。 当嵌入膜中时，这些线圈的富含氧气的静电内部孔可为离子通过膜提供通道。 另外，在膜结合的折叠剂的内部孔中存在许多酰胺氧原子作为氢键受体，可以使多种氢键与糖和糖醇具有不同强度的相互作用。 这种相互作用是动态和瞬态的，尤其是在水中，导致离子电流通过跨膜孔的扰动。 根据它们的结构和特性，不同类型的碳水化合物扰动离子通过孔的流动不同，可以非常敏感地检测到这一点。 通过测量在不同糖及其衍生物存在下跨膜离子电导的变化，该研究旨在监测和理解在非平衡条件下这些分子的分子识别。 具体而言，这项工作的重点是合成不同长度（8mer，16mer，32mer和64mer）的寡酰胺，并检查各种糖和糖醇对跨膜离子电流的效果通过孔形成折叠剂。进行基于囊泡的测定方法是为了对所选碳水化合物的影响进行快速但定性的筛查，然后进行单通道电流的测量，以揭示不同碳水化合物分子的特征。这奖反映了NSF的立法任务，并认为通过基金会的智力效果和广阔的critiation和Broadiatia的评估值得评估。

项目成果

Stable pseudo[3]rotaxanes with strong positive binding cooperativity based on shape-persistent aromatic oligoamide macrocycles

基于形状持久的芳香低聚酰胺大环，具有强正结合协同性的稳定假[3]轮烷

• DOI: 10.1039/d1cc05193h
• 发表时间: 2021
• 期刊: Chemical Communications
• 影响因子: 4.9
• 作者: Sobiech, Thomas A.;Zhong, Yulong;Sánchez B., Laura S.;Kauffmann, Brice;McGrath, Jillian K.;Scalzo, Christina;Miller, Daniel P.;Huc, Ivan;Zurek, Eva;Ferrand, Yann
• 通讯作者: Ferrand, Yann

Ultrasensitive liposome-based assay for the quantification of fundamental ion channel properties

基于超灵敏脂质体的测定，用于定量基本离子通道特性

• DOI: 10.1016/j.aca.2020.03.044
• 发表时间: 2020-05-22
• 期刊: ANALYTICA CHIMICA ACTA
• 影响因子: 6.2
• 作者: Shen，Yi;Zhong，Yulong;Shao，Zhifeng
• 通讯作者: Shao，Zhifeng

Cavity-containing aromatic oligoamide foldamers and macrocycles: progress and future perspectives

• DOI: 10.1039/d2ob01467j
• 发表时间: 2022-08-23
• 期刊: ORGANIC & BIOMOLECULAR CHEMISTRY
• 影响因子: 3.2
• 作者: Sobiech，Thomas A.;Zhong，Yulong;Gong，Bing
• 通讯作者: Gong，Bing