SBIR Phase II: Development of Broadly Efficacious Ribonucleic Acid Interference Triggers for Pesticides

SBIR 第二阶段：开发广泛有效的农药核糖核酸干扰触发器

基本信息

批准号：
1853008
负责人：
Juan Arhancet
金额：
$ 74.53万
依托单位：
NanoSUR LLC
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-03-01 至 2023-07-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1853008&HistoricalAwards=false
关键词：
SBIR Phase II Development Broadly

项目摘要

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) Phase II project will be to provide farmers with a new class of biotechnology-based pesticides for more affordable and environmentally benign crop production by addressing an unmet need: an efficacious, selective and safe pesticide. The project will focus on a pesticide for the diamondback moth (DBM), a pest that damages Brassica crops such as canola, cabbage, cauliflower and collards. It is the most costly invasive crop pest worldwide, for which there is no effective solution with acceptable resistance management. DMB has developed resistance to more than 90 active ingredients in conventional insecticides, i.e., almost every insecticide class known. If successful, this biotechnology platform will contribute to sustainable food production and enhanced biodiversity with minimal use of non-selective traditional pesticides associated with human health costs. The goal is to develop a product that is efficacious, selective, and provides improved resistance management against field populations of DMB.The intellectual merit of this SBIR Phase II project is the development of RNAi-based pesticide for the treatment of diamondback moth (Plutella xylostella) infestations in cruciferous crops. In the last five years, there have been significant advances in the methods for the production of RNAi triggers that allow the manufacturing of these materials in large quantities and at a low price, which is required for broad agricultural applications. Topical RNAi-based pesticides have been demonstrated in the lab to be very selective with respect to the intended target. They also may be readily modified to combat developed pesticide resistance by target pests. However, due to low efficacy at economically attractive application rates, very few pests and no Lepidopteran insects are currently in the RNAi list of commercial targets. This project specifically addresses the challenges related to RNAi efficacy, and the development of the proposed technology could be applied to Lepidopteran control and other biotechnology applications. The outcome of the proposed research would provide the first example of a scalable RNAi-based insecticide targeting Plutella xylostella or any Lepidopteran.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项小型企业创新研究（SBIR）第二阶段项目的更广泛的影响/商业潜力将是为农民提供新的基于生物技术的农药，以通过满足未满足的需求：有效，选择性和安全的农药，以实现更实惠和环境的农作物生产。该项目将重点放在响尾蛇飞蛾（DBM）的农药上，该虫害会损害诸如低芥酸菜籽，卷心菜，花椰菜和call虫等甘蓝植物。它是全球最昂贵的侵入性作物害虫，对于可接受的阻力管理，没有有效的解决方案。 DMB对常规杀虫剂（即几乎所有已知的杀虫剂类别）产生了对90多种活性成分的抗性。如果成功，该生物技术平台将有助于可持续的粮食生产和增强生物多样性，而最少使用与人类健康成本相关的非选择性传统农药。目的是开发一种有效，有选择性的产品，并为DMB的现场种群提供了改进的抵抗力管理。该SBIR II期项目的智力优点是开发基于RNAi的农药来治疗钻石蛾（Plutella xylostella）在十字花科crops中的侵袭。在过去的五年中，生产RNAi触发器的方法取得了重大进展，这些方法允许大量和低廉的价格生产这些材料，这是广泛的农业应用所必需的。在实验室中已经证明了基于RNAi的农药相对于预期目标非常有选择性。它们也可能很容易修改，以应对目标害虫的开发农药耐药性。但是，由于在经济上有吸引力的应用率下的效力低下，目前很少有害虫和鳞翅目昆虫在商业目标的RNAi列表中。该项目专门解决了与RNAi疗效有关的挑战，并且所提出的技术的开发可以应用于鳞翅目控制和其他生物技术应用。拟议的研究的结果将提供一个基于RNAi的杀虫剂靶向木壳虫或任何鳞翅目的第一个例子。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响审查标准通过评估来进行评估的。