Collaborative Research: Evolutionary, biochemical and biogeochemical responses of marine cyanobacteria to warming and iron limitation interactions

合作研究：海洋蓝藻对变暖和铁限制相互作用的进化、生化和生物地球化学反应

• 批准号: 1850719
• 负责人: Mak Saito
• 金额: $ 49.97万
• 依托单位: Woods Hole Oceanographic Institution
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-04-01 至 2023-09-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1850719&HistoricalAwards=false
• 关键词: Collaborative; Research; Evolutionary; biochemical; biogeochemical

The oceans absorb much of the heat generated by human activities, and this warming of the surface ocean has consequences for important groups of marine organisms. Marine cyanobacteria are one such key group of organisms, since they supply much of the essential carbon and nitrogen that supports nearly all the rest of the marine food web. Currently, the growth of cyanobacteria is mostly constrained by scarce supplies of the micronutrient element iron, but they are also very sensitive to the ongoing increases in seawater temperature. Preliminary results suggest that warming could partly mitigate the negative effects of iron limitation on marine cyanobacteria. This project examines in depth how these interactions between warming and iron limitation will affect the future ocean carbon and nitrogen cycles, using laboratory culture experiments showing how cyanobacteria respond to simultaneously changing temperature and iron supplies. Both short-term response studies and long-term evolutionary experiments testing for adaptation use a comprehensive set of molecular biology tools targeting genes to proteins. The final goal is to apply the results of these experiments to improve quantitative models predicting how the ocean's carbon and nitrogen cycles, biological productivity, and living resources will respond to a warming future climate. Two graduate students, a postdoc and 3-4 underrepresented undergraduate researchers are supported, and the investigators also mentor summer science interns from largely Hispanic local high schools. The physiology, biochemistry and biogeography of nitrogen-fixing cyanobacteria and unicellular picocyanobacteria are strongly influenced by temperature, subjecting them to intense selective pressure as the modern ocean steadily warms up. These groups have likewise been rigorously selected under chronic iron (Fe) scarcity, and the availability of this crucial micronutrient is also changing with a shifting climate. This project examines short-term acclimation and long-term evolutionary responses of Fe-stressed marine cyanobacteria to a warmer environment. Preliminary data show that Iron Use Efficiencies (IUE, mols N fixed.hr-1 mol cellular Fe-1) of Fe-limited Trichodesmium increase 4 to 5-fold with a 5oC temperature increase, allowing the cells to much more efficiently leverage scarce available Fe supplies to grow and fix nitrogen. This means that warming can to a large degree mitigate the negative effects of Fe limitation on Trichodesmium, resulting in a modelled 22% increase in global nitrogen fixation by 2100 in a warmer climate. This project aims to uncover the cellular biochemical mechanisms involved in this Fe-limitation/thermal IUE effect in a four-year experimental evolution study of the diazotrophs Trichodesmium and Crocosphaera and the picocyanobacteria Synechococcus and Prochlorococcus, under a multi-variate selection matrix of temperature and Fe availability. The objectives are to 1) Assess the long-term adaptive responses of fitness, IUE and physiology to Fe limitation and warming interactions in these four major cyanobacterial groups; 2) Determine the molecular and biochemical mechanisms behind the surprising Fe/warming interactive effect on IUE using genomics, transcriptomics and quantitative proteomics coupled with 'metalloproteomics' determinations of Fe content in critical proteins; 3) Compare and contrast acclimation and adaptation responses to Fe limitation and warming in key cyanobacteria taxa, and 4) Integrate results using a published biogeochemical modeling approach to assess global consequences for marine productivity and nitrogen fixation. This project offers a mechanistic and predictive understanding of adaptation to Fe and warming co-stressors in a rapidly changing future ocean environment for some of the most important photoautotrophic functional groups in the ocean.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

海洋吸收了人类活动产生的许多热量，而这种地表海洋的变暖对重要的海洋生物群产生了影响。海洋蓝细菌就是这样的关键生物群，因为它们提供了许多基本的碳和氮，这些碳和氮几乎支持了所有其他海洋食品网。当前，蓝细菌的生长主要受微量营养素铁的稀缺供应的限制，但它们也对海水温度的持续升高也非常敏感。初步结果表明，变暖可以部分减轻铁限制对海洋蓝细菌的负面影响。该项目深入探讨了使用实验室培养实验表明蓝细菌对同时变化的温度和铁供应的响应方式，研究了变暖和铁限制之间的这些相互作用将如何影响未来的海洋碳和氮气周期。适应性的短期反应研究和长期进化实验测试都使用了一组靶向基因靶向蛋白质的分子生物学工具。最终目标是应用这些实验的结果来改善定量模型，以预测海洋的碳和氮循环，生物生产力和生命资源如何应对未来气候变暖。支持两名研究生，一名博士后和3-4个代表性的本科生研究人员，研究人员还指导来自西班牙裔当地高中的夏季科学实习生。氮固定蓝细菌和单细胞皮基氨基细菌的生理学，生物化学和生物地理学受到温度的强烈影响，随着现代海洋稳步加热，它们的选择性压力很大。这些群体同样是在慢性铁（FE）稀缺下进行严格选择的，并且这种关键的微量营养素的可用性也随着气候变化而变化。该项目研究了FE胁迫海洋蓝细菌对温暖环境的短期适应和长期进化反应。初步数据表明，Fe限制的毛刺trichodesmium的使用效率（iue，mols n固定。HR-1摩尔细胞Fe-1）随着5oC温度的升高增加了4至5倍，使细胞更有效地利用可用的FE供应来生长和固定氮。这意味着，变暖可以在很大程度上减轻Fe限制对毛trichodesmium的负面影响，从而导致在温暖的气候下，将全球氮固定的22％增加了2100。该项目旨在揭示在多核酸菌和鳄鱼果皮的四年实验进化研究中，在多个多体温度和fe fe avalix下，在多核酸菌和果氯环的四年实验进化研究中涉及的细胞生物化学机制。目标是1）评估适应性和生理学对FE限制和变暖相互作用的长期适应性反应； 2）确定使用基因组学，转录组学和定量蛋白质组学以及“金属蛋白质组学”在临界蛋白中测定的“金属蛋白质组学”测定的令人惊讶的Fe/变暖互动效应背后的分子和生化机制； 3）对关键蓝细菌分类群中FE限制和变暖的适应和对比度的适应和适应反应，以及4）使用已发表的生物地球化学建模方法整合结果，以评估对海洋生产力和氮固定的全球后果。 该项目为对海洋中一些最重要的光自营养官能团的未来海洋环境中的适应和变暖的共同压力提供了一种机械和预测性的理解。该奖项反映了NSF的法定任务，并认为通过基金会的知识和更广泛的影响，通过评估来审查CRITERIA，并被认为是通过评估的支持。

项目成果

Dynamic diel proteome and daytime nitrogenase activity supports buoyancy in the cyanobacterium Trichodesmium.

• DOI: 10.1038/s41564-021-01028-1
• 发表时间: 2022-02
• 期刊: Nature microbiology
• 影响因子: 28.3

Online Nanoflow Two-Dimension Comprehensive Active Modulation Reversed Phase-Reversed Phase Liquid Chromatography High-Resolution Mass Spectrometry for Metaproteomics of Environmental and Microbiome Samples.

• DOI: 10.1021/acs.jproteome.1c00588
• 发表时间: 2021-09-03
• 期刊: JOURNAL OF PROTEOME RESEARCH
• 影响因子: 4.4
• 作者: McIlvin, Matthew R.;Saito, Mak A.
• 通讯作者: Saito, Mak A.

Efficient zinc/cobalt inter‐replacement in northeast Pacific diatoms and relationship to high surface dissolved Co : Zn ratios

东北太平洋硅藻中有效的锌/钴相互置换及其与高表面溶解钴：锌比率的关系

• DOI: 10.1002/lno.11471
• 发表时间: 2020
• 期刊: Limnology and Oceanography
• 影响因子: 4.5
• 作者: Kellogg, Riss M.;McIlvin, Matthew R.;Vedamati, Jagruti;Twining, Benjamin S.;Moffett, James W.;Marchetti, Adrian;Moran, Dawn M.;Saito, Mak A.
• 通讯作者: Saito, Mak A.

Abundant nitrite-oxidizing metalloenzymes in the mesopelagic zone of the tropical Pacific Ocean

• DOI: 10.1038/s41561-020-0565-6
• 发表时间: 2020-05
• 期刊: Nature Geoscience
• 影响因子: 18.3
• 作者: M. Saito;M. McIlvin;D. Moran;A. Santoro;C. Dupont;P. Rafter;Jaclyn K. Saunders;D. Kaul;C. Lamborg;Marian B. Westley;F. Valois;J. Waterbury

Adaptive responses of marine diatoms to zinc scarcity and ecological implications.

• DOI: 10.1038/s41467-022-29603-y
• 发表时间: 2022-04-14
• 期刊: Nature communications
• 影响因子: 16.6