Intergenerational impact of violence exposure during pregnancy on epigenetic change

怀孕期间暴力暴露对表观遗传变化的代际影响

• 批准号: 1849379
• 负责人: Connie Mulligan
• 金额: $ 34.83万
• 依托单位: University of Florida
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-08-01 至 2025-07-31
• 项目状态: 未结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1849379&HistoricalAwards=false
• 关键词: Intergenerational; impact; violence; exposure; during

Trauma that is experienced by a pregnant woman can affect the physiology of her offspring, sometimes decades into the future. The impact of trauma may be caused by changes to our genome that alter the way in which genes are expressed and proteins are produced. This project will test the hypothesis that violence experienced by a pregnant woman may produce changes in the genomes of the mother, her child, and her grandchild. This study is a global, interdiscriplinary collaboration that will provide a better understanding of how humans adapt to adversity, and how these adaptations may persist far into the future, long after the adverse conditions have ended. The results of this research will be communicated to the public and scientific community through presentations and publications, student exchanges, and outreach activities to local communities and policymakers. This project will train one postdoctoral assistant and 3-4 undergraduate students, many of whom are from groups underrepresented in STEM research fields.In this study, a unique set of biological samples have been collected and will be assayed for changes to the genome, specifically the addition of DNA methyl groups that can change the expression of a gene. Three generation families have been recruited who have been exposed to violence in different generations; specifically, grandmothers in group 1 were exposed while pregnant, mothers in group 2 were exposed while pregnant, and group 3 are control familes who were not exposed. Biological samples from the mothers and two children (one child was exposed in utero and one child will serve as a control) will be analyzed using cutting-edge methods to assay for DNA methylation changes. Associations between changes to DNA methylation and exposure to violence in each generation will be tested, while controlling for age at time of exposure, age at sample collection, sex, and additional adverse exposures, such as lack of food or water or medical care.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

孕妇经历的创伤会影响她后代的生理，有时在未来几十年。创伤的影响可能是由于我们的基因组的变化引起的，这种变化改变了表达基因并产生蛋白质的方式。该项目将检验以下假设：孕妇经历的暴力可能会改变母亲，孩子和她的孙子的基因组。这项研究是一项全球性的互助合作，将更好地理解人类如何适应逆境，以及这些适应能力如何在不良条件结束之后很长一段时间内持续到未来。这项研究的结果将通过演讲和出版物，学生交流以及向当地社区和政策制定者的演讲，学生交流以及外展活动传达给公众和科学界。该项目将培训一名博士后助理和3-4名本科生，其中许多是来自STEM研究领域中代表性不足的小组。在这项研究中，已经收集了一组独特的生物样品，并将被分析以改变基因组，特别是对基因组的更改，特别是添加可以改变基因表达的DNA甲基。已经招募了三代家庭，他们在不同世代遭受暴力行为；具体来说，第1组中的祖母在怀孕期间暴露了，第2组的母亲在怀孕时暴露了，第3组是对照家族，未暴露。将使用尖端的方法分析来自母亲和两个孩子的生物样本（在子宫内暴露了一个孩子，一个孩子将作为对照）进行分析，以分析DNA甲基化变化。将测试对DNA甲基化变化与暴露暴露之间的关联，同时控制暴露时的年龄，样本收集年龄，性别和其他不良暴露，例如缺乏食物或水或医疗服务。奖项反映了NSF的法定任务，并通过使用基金会的智力优点和更广泛的影响审查标准评估值得支持。