BTT EAGER: A system for the production of haploid inducer lines and cytoplasmic male sterile doubled haploids for efficient hybrid production

BTT EAGER：用于生产单倍体诱导系和细胞质雄性不育双单倍体的系统，用于高效杂交生产

• 批准号: 1844304
• 负责人: Patrick Krysan
• 金额: $ 30万
• 依托单位: University of Wisconsin-Madison
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-03-01 至 2023-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1844304&HistoricalAwards=false
• 关键词: BTT; EAGER; system; production; haploid

Hybrid cultivars are a cornerstone of modern crop production. Seed companies spend many years developing elite, inbred parental lines that are used to produce the hybrid seed that farmers plant each season. A major bottleneck in the plant breeding cycle is caused by the fact that it takes many years to produce these inbred parental lines. The aim of this project is to develop a shortcut that allows inbred parental lines to be produced in a single year. To accomplish this goal, the investigators plan to use gene editing techniques to generate novel onion plants that greatly accelerate the process of inbred line development. The strategy deployed in this work should also be broadly applicable to other crops, making it of general use to the plant breeding community. This work will be carried out in collaboration with Dr. Stephen Jackson at the University of Warwick.The investigators will use CRISPR/Cas9 methods to introduce genetic variation into the CENH3 gene of onion in order to produce haploid-inducer lines. A haploid-inducer line is a plant from which a significant fraction of the seed produced by cross-pollination is haploid. Upon chemically-induced chromosome doubling, these haploid progeny become "instant inbred" lines that can be immediately used for hybrid seed production. The research team at the University of Wisconsin will use T-DNA transformation and particle bombardment methods to deliver the gene editing reagents into onion somatic embryos, while the group at the University of Warwick will use tobacco rattle virus (TRV) expression vectors. Deploying a variety of gene-editing strategies in parallel should allow the investigators to quickly determine which approach is the most effective for efficiently producing modified CENH3 alleles. In order for doubled haploid technology to be commercially useful for hybrid seed production, it is essential that the strategies for producing doubled haploids are compatible with cytoplasmic male sterile breeding systems. For this reason, this project will integrate the production of haploid inducer lines within a cytoplasmic male sterile breeding framework in order to maximize the practical benefit and applicability to breeders.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

混合品种是现代作物生产的基石。种子公司花了很多年来开发精英，近亲的父母系列，用于生产每个季节种植农民的混合种子。植物繁殖周期中的主要瓶颈是由于生产这些近交系列的事实所致。 该项目的目的是开发一个捷径，该快捷方式允许在一年内生产近亲的父母线条。 为了实现这一目标，研究人员计划使用基因编辑技术来产生新型的洋葱植物，从而大大加速了近交系的发展过程。 这项工作中部署的策略也应广泛适用于其他农作物，使其对植物育种社区进行了普遍用途。这项工作将与沃里克大学的Stephen Jackson博士合作进行。调查人员将使用CRISPR/CAS9方法将遗传变异引入洋葱的Cenh3基因中，以产生单倍体诱导者线。单倍体诱导剂线是一种植物，从中，交叉授粉产生的种子很大一部分是单倍体。 化学诱导的染色体加倍后，这些单倍体后代成为“瞬间近交”线，可立即用于杂交种子的产生。 威斯康星大学的研究团队将使用T-DNA转化和粒子轰击方法将基因编辑试剂传递到洋葱体细胞胚胎中，而沃里克大学的小组将使用烟草蛇毒病毒（TRV）表达矢量。 在并行中部署各种基因编辑策略应使研究人员能够快速确定哪种方法是有效产生修改的CENH3等位基因最有效的方法。 为了使双倍技术对杂种种子的产生具有商业用处，至关重要的是，产生双倍的单倍体的策略与细胞质雄性雄性无菌育种系统兼容。因此，该项目将在细胞质的男性无菌育种框架内整合单倍体诱导剂线的生产，以最大程度地提高对育种者的实际收益和适用性。该奖项反映了NSF的法定任务，并认为通过使用该基金会的知识分子和更广泛的影响来审查Criteria，它被认为是值得通过评估的支持。