RoL:EAGER:DESYN-C3: Engineering Microbial Differentiation

RoL:EAGER:DESYN-C3：工程微生物分化

基本信息

批准号：
1844289
负责人：
Corey Wilson
金额：
$ 30万
依托单位：
Georgia Tech Research Corporation
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-09-01 至 2020-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1844289&HistoricalAwards=false
关键词：
RoL EAGER DESYN C3 Engineering

项目摘要

Human stem cells can become different cell types (muscle, blood, brain) through a process called differentiation. Imagine differentiation as a series of calculations that the cell performs to determine what type of cell will survive best in a given environment in the body. The cell needs to remember the answer to the previous calculations in order to make the next calculation, so it needs a permanent memory of some type. Bacterial species do not change from one cell type to another because they do not have the capability to remember. Creating memories could allow a population of bacteria to calculate the best solution to a problem. For example, the population would be able to decide the best strategy to remove toxins from drinking water, or to produce a therapeutic vaccine. This project will create bacterial memory structures and will evaluate the ability of the bacteria to perform different calculations. The project will offer female and under-represented minority students research opportunities. A workshop for training undergraduates in the research techniques used will also be developed.The goal of this project is to create genetic memory. The approach taken is to develop genetic structures (e.g., promoters, reading frames and terminators) that can be rapidly rearranged in response to stimuli. The response will therefore be inherited, persisting long after the removal of the stimulus that created it. For this development to be significant, two or more different switches must be created so that the cell is capable of performing logical calculations, similar to computer programs, that allow for more than two distinct solutions (e.g. on or off). This could ultimately lead to the development of schemes and workflows for scalable genetic MEMORY structures and dynamic cellular programming. Testing and optimization of the appropriate subsystems will be conducted and will be used to construct a full-scale MEMORY structure (i.e., a biological calculator) and the subsequent differentiation architectures that will serve as the processing unit for nine varieties of synthetic microbial stem cells.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

通过称为分化的过程，人类干细胞可以变成不同的细胞类型（肌肉，血液，大脑）。想象一下，分化是细胞执行的一系列计算，以确定在体内给定环境中哪种类型的细胞将在哪种类型中生存。该单元格需要记住先前计算的答案，以便进行下一个计算，因此它需要对某种类型的永久记忆。细菌物种不会从一种细胞类型变为另一种细胞类型，因为它们没有记住的能力。创造记忆可以使细菌种群计算出问题的最佳解决方案。例如，人口将能够决定从饮用水中去除毒素或生产治疗性疫苗的最佳策略。该项目将创建细菌记忆结构，并评估细菌执行不同计算的能力。该项目将为女性和代表性不足的少数民族学生的研究机会提供。还将开发用于使用研究技术的培训本科生的研讨会。该项目的目的是创建遗传记忆。所采用的方法是开发遗传结构（例如启动子，阅读框架和终结剂），可以迅速重新排列刺激。因此，响应将被继承，在去除创建它的刺激后很长时间持续存在。为了使该开发很重要，必须创建两个或更多不同的开关，以便单元格能够执行与计算机程序相似的逻辑计算，从而允许使用两个以上不同的解决方案（例如开或关）。这最终可能导致方案和工作流程的发展，以进行可扩展的遗传记忆结构和动态细胞编程。将进行适当子系统的测试和优化，并将用于构建全尺度的记忆结构（即生物计算器）和随后的差分体系结构，这些结构将作为九种合成微生物干细胞的九种处理单元，这些奖项通过NSF的法规及其范围进行了评估。标准。