RoL: FELS: RAISE: A Phylogenomically-Based Bioinspired Robotic Model Approach to Addressing the Evolution of Terrestrial Locomotion

RoL：FELS：RAISE：一种基于系统发育学的仿生机器人模型方法来解决陆地运动的进化问题

• 批准号: 1839915
• 负责人: Brooke Flammang
• 金额: $ 99.75万
• 依托单位: New Jersey Institute of Technology
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2019
• 资助国家: 美国
• 起止时间: 2019-01-01 至 2024-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1839915&HistoricalAwards=false
• 关键词: RoL; FELS; RAISE; Phylogenomically; Based

One clear example of a Rule of Life is evolutionary convergence, the occurrence of the same or extremely similar adaptations in independent lineages of species on the Tree of Life. The recent discovery of a blind cavefish that walks and climbs waterfalls with a salamander-like gait and has evolved a robust pelvic girdle (a kind of hip) offers an extraordinary opportunity to study, in living fishes, a pivotal event in evolutionary history. That event, the vertebrate invasion of land, hinged on the evolution of fins to limbs that enabled the first vertebrates to walk on land. The fossil record of this transition is scarce, and extant fishes were thought to lack the morphological features necessary for studying the origin of quadrupedal walking. This research will provide a unique opportunity to understand, through phylogenetic analysis and mathematical and robotic modeling, the mechanism underlying a major event in the history of life: how fishes were able to transition to a terrestrial lifestyle and evolve into the tetrapods. The broader impacts of this project will promote teaching, training, and learning for high school students in diverse and underprivileged communities. The project will also provide opportunities for graduate and undergraduate research training and make important contributions to public science education. This project was co-funded by the Biology Directorate and the Physics of Living Systems Program in the Physics Division. The proposed work will identify the genomic, morphological, and mechanical qualities that enable a fish to walk on land. Advancing previous work on the functional morphology and kinematics of terrestrial walking in the blind cave fish Cryptotora thamicola, the proposed research will examine the phylogenomic relationships, morphology, biomechanics, and walking performance from an evolutionary perspective among balitorid loaches. Genomic sequencing will determine convergent genetic markers known to be involved in pelvic girdle formation in other walking vertebrates. Morphological examinations will reveal how functional phenotypic adaptations can be formed by major genomic changes. A biorobotic model organism, validated through comparison with our experimental biomechanical data, will be used to take a modular approach to examining convergence and answering questions on the effect of pelvic morphology on walking performance in extinct fin-to-limb transitional forms and modern forms. The work proposed will discriminate the fundamental physical properties which are inherent to the transition to terrestrial walking from an aquatic form. The evolution of the terrestrial pelvic girdle was critical to the diversification of amphibians, reptiles, birds and mammals; the proposed work uses a convergent fish model to study the evolution of anatomical structures that support the vertebrate body against gravity and show how modulation of muscular control can produce a tetrapodal gait within a given range of skeletal morphologies. This research will inform scientific understanding of the mechanisms underlying the convergent evolution of morphological innovation, allowing development of a unifying principle for mass support in tetrapods based on the genomics, basic physics, and mechanics that define the pelvic girdle contribution to quadrupedal walking.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

生命规则的一个明显例子是进化趋同，即生命之树上物种的独立谱系中发生相同或极其相似的适应。最近发现了一种盲眼洞穴鱼，它以类似蝾螈的步态行走和攀爬瀑布，并进化出了坚固的骨盆带（一种臀部），这为研究现存鱼类这一进化史上的关键事件提供了绝佳的机会。脊椎动物入侵陆地这一事件取决于鳍向四肢的进化，这使得第一批脊椎动物能够在陆地上行走。这种转变的化石记录很少，现存的鱼类被认为缺乏研究四足行走起源所需的形态特征。这项研究将提供一个独特的机会，通过系统发育分析以及数学和机器人建模，了解生命史上重大事件的潜在机制：鱼类如何能够过渡到陆地生活方式并进化成四足动物。该项目的更广泛影响将促进多元化和贫困社区高中生的教学、培训和学习。该项目还将为研究生和本科生提供研究培训机会，为公共科学教育做出重要贡献。该项目由生物理事会和物理部生命系统物理项目共同资助。 拟议的工作将确定鱼类能够在陆地上行走的基因组、形态和机械特性。这项研究将推进先前关于盲穴鱼 Cryptotora thamicola 陆地行走的功能形态和运动学的研究，将从进化的角度研究巴里托科泥鳅的系统发育关系、形态、生物力学和行走性能。基因组测序将确定已知参与其他行走脊椎动物骨盆带形成的趋同遗传标记。形态学检查将揭示主要基因组变化如何形成功能表型适应。通过与我们的实验生物力学数据进行比较进行验证的生物机器人模型有机体将用于采用模块化方法来检查收敛性并回答有关骨盆形态对灭绝的鳍到四肢过渡形式和现代形式步行性能的影响的问题。拟议的工作将区分从水生形式向陆地行走过渡所固有的基本物理特性。陆地骨盆带的进化对于两栖动物、爬行动物、鸟类和哺乳动物的多样化至关重要。拟议的工作使用收敛鱼模型来研究支持脊椎动物抵抗重力的解剖结构的演化，并展示肌肉控制的调节如何在给定的骨骼形态范围内产生四足步态。这项研究将有助于科学地理解形态创新趋同进化的机制，从而基于基因组学、基础物理学和力学来制定四足动物质量支撑的统一原则，这些原则定义了骨盆带对四足行走的贡献。反映了 NSF 的法定使命，并通过使用基金会的智力价值和更广泛的影响审查标准进行评估，被认为值得支持。

项目成果

Catfishes of the Genus Glyptothorax (Siluriformes: Sisoridae) in the Mae Klong River Basin, Thailand, with Taxonomic Implications for Several Southeast Asian Species

• DOI: 10.1643/i2022045
• 发表时间: 2023-06
• 期刊: Ichthyology & Herpetology
• 影响因子: 1.5
• 作者: David A. Boyd;S. Tongnunui;L. Page

Bioinspired Design in Research: Evolution as Beta-Testing

研究中的仿生设计：作为 Beta 测试的进化

• DOI: 10.1093/icb/icac134
• 发表时间: 2022
• 期刊: Integrative And Comparative Biology
• 影响因子: 2.6
• 作者: Flammang, Brooke E.

Revision of the Genus Henicorhynchus, with a Revised Diagnosis of Gymnostomus (Cyprinidae: Labeoninae)

• DOI: 10.1643/ci-19-304
• 发表时间: 2020-08
• 期刊: Copeia
• 影响因子: 2.6
• 作者: P. Ciccotto;L. Page

Garra surinbinnani, a new species of labeonine from the Mae Khlong basin of Thailand (Teleostei: Cyprinidae).

Garra surinbinnani，来自泰国 Mae Khlong 盆地的 Labeonine 新种（Teleostei：鲤科）。

• DOI: 10.23788/ief-1117
• 发表时间: 2019
• 期刊: Ichthyological Exploration of Freshwaters
• 影响因子: 1
• 作者: Page, L.;Ray, B. R.;Boyd, D. A.;Randall, Z. S.
• 通讯作者: Randall, Z. S.

They like to move it (move it): walking kinematics of balitorid loaches of Thailand

他们喜欢移动它（移动它）：泰国巴里托里德泥鳅的行走运动学

• DOI: 10.1242/jeb.242906
• 发表时间: 2022
• 期刊: Journal of Experimental Biology
• 影响因子: 2.8
• 作者: Crawford, Callie H.;Webber-Schultz, Amani;Hart, Pamela B.;Randall, Zachary S.;Cerrato-Morales, Cristian;Kellogg, Audrey B.;Amplo, Haley E.;Suvarnaraksha, Apinun;Page, Lawrence M.;Chakrabarty, Prosanta
• 通讯作者: Chakrabarty, Prosanta