MRI: Development of a time-resolved, high resolution nonlinear optical microscope for interfacial studies

MRI：开发用于界面研究的时间分辨高分辨率非线性光学显微镜

基本信息

批准号：
1828421
负责人：
Eric Borguet
金额：
$ 107.45万
依托单位：
Temple University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2018
资助国家：
美国
起止时间：
2018-10-01 至 2023-09-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1828421&HistoricalAwards=false
关键词：
MRI Development time resolved resolution

项目摘要

This award is supported by the Major Research Instrumentation (MRI), the Chemistry Research Instrumentation (CRIF) and the Chemical Measurement and Imaging (CMI) Programs. Professor Eric Borguet from Temple University and colleague Hai-Lung Dai have developed a time-resolved, high resolution optical microscope. This microscope employs a laser to probe samples such as protein membranes in living cells or nanomaterials such as the surfaces of gold nanoparticles. The interaction of the laser with the sample produces an image of the material under study. Being able to image interfaces with time resolution and molecular specificity is important to the understanding of a wide variety of biological, chemical and materials systems. For example, it is an important approach for examining interfacial water molecules. This new microscope affords the capability to record images illustrating water structure at the interface between a biological cell and a substrate which is information critical for understanding cell adhesion. It is used to monitor molecular adsorption (for example drugs) and transport at membranes of living cells. Graduate students are involved in the development and testing of the instrument, receiving training in an important skill sought by commercial instrument firms. Once the instrument is available for use, undergraduates will receive training in its usage in laboratory courses. Multiple investigators from various universities are utilizing this resource.This research is aimed at enhancing research and education at all levels. The microscope records images of samples at millisecond time intervals and with submicron spatial resolution producing second order nonlinear optical signals. This microscope uses an optical fiber based laser system which provides high repetition rate, high pulse energy, ultrafast laser pulses in a broad wavelength range in the visible, and IR to facilitate resonantly enhanced second harmonic generation (SHG) and sum frequency generation (SFG) from a variety of materials and molecules at surfaces/interfaces. The microscope enables imaging of molecular transport with region-specificity at living cell membranes. In addition, it permits examination of non-specific cell adhesion using nonlinear vibrational microscopy and the observation of molecular interactions at the surfaces of colloidal objects. The instrumentation is also used for the study of two-dimensional materials at interfaces using nonlinear optical microscopy and for determining inner and outer nuclear membrane protein orientation and distribution in live cells. The microscope also serves researchers probing surface-bound ligands on gold nanoparticles and visualizing electronic symmetry breaking in quantum materials. It is also being used for the evaluation of cartilage tissue engineering strategies by IR imaging, and for imaging of leukocyte-endothelial interaction in a novel organ-on-a-chip system using time-resolved, high-resolution imaging.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

该奖项得到了主要的研究工具（MRI），化学研究工具（CRIF）和化学测量和成像（CMI）计划的支持。来自坦普尔大学的埃里克·鲍尔圭（Eric Borguet）教授和同事海伦·戴（Hai-Lung Dai）开发了时间分辨，高分辨率的光学显微镜。该显微镜使用激光来探测活细胞中的蛋白质膜或纳米材料（例如金纳米颗粒表面）中的样品。激光与样品的相互作用产生正在研究的材料的图像。能够通过时间分辨率和分子特异性对界面进行成像，对于理解各种生物，化学和材料系统的理解很重要。例如，这是检查界面水分子的重要方法。该新的显微镜具有记录图像的能力，以说明生物细胞和底物之间界面处的水结构，这对于理解细胞粘附至关重要。它用于监测活细胞膜上的分子吸附（例如药物）和运输。研究生参与了乐器的开发和测试，接受了商业仪器公司寻求的重要技能的培训。一旦可以使用该工具，本科生将接受其在实验室课程中使用的培训。来自各种大学的多个研究人员正在利用此资源。这项研究旨在增强各级研究和教育。显微镜以毫秒的时间间隔记录样品的图像，并以亚微米空间分辨率产生二阶非线性光学信号。该显微镜使用基于光纤的激光系统，该激光系统可在可见的广泛波长范围内提供高重复速率，高脉冲能量，超快激光脉冲，而IR可促进从表面/Interface的各种材料和分子中共振增强的第二次谐波产生（SHG）和总和频率产生（SHG）。显微镜可以在活细胞膜上以区域特异性进行分子转运的成像。此外，它允许使用非线性振动显微镜检查非特异性细胞粘附，并在胶体物体表面上观察分子相互作用。该仪器还用于使用非线性光学显微镜在界面上研究二维材料，并确定活细胞中的内部和外核膜蛋白方向和分布。显微镜还为研究人员提供服务，在金纳米颗粒上探测表面结合的配体，并可视化量子材料中的电子对称性破坏。它也用于通过IR成像来评估软骨组织工程策略，以及使用时间分解的高分辨率成像的新型器官芯片系统中白细胞 - 内皮相互作用的成像。该奖项颁发了NSF的法定任务，反映了NSF的法定任务，并通过评估基金会的范围进行了评估和宽广的构成效果。