CAREER: Three Region nLC-MS/MS Analysis: A Novel and Rapid Approach to Evaluate Molecular Penetration

职业：三区域 nLC-MS/MS 分析：一种评估分子渗透的新颖且快速的方法

• 批准号: 1827084
• 负责人: Amanda Hummon
• 金额: $ 21.04万
• 依托单位: Ohio State University
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-01-01 至 2020-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1827084&HistoricalAwards=false
• 关键词: CAREER; Three; Region; nLC; MS

In this CAREER project, Professor Amanda Hummon and her students at the University of Notre Dame will develop a new mass spectrometric methodology for the analysis of chemical compounds in cell cultures. The approach will provide much greater chemical and spatial information in a faster and higher-throughput fashion than is currently available with any other methodology. As part of this CAREER educational program, Professor Hummon and her students will develop a program focused on the recruitment and retention of women in STEM fields by organizing lab tours with three local Girl Scout troops per year and participation in two forums per semester to discuss issues that disproportionally affect women. This program will encourage the participation of younger female students in science careers.This research will study the penetration of chemical compounds into three dimensional cell culture models via mass spectrometry. Using a combination of serial trypsinization and nano Liquid Chromatography-Electrospray Tandem Mass Spectrometry (nLC-MS/MS), the distribution of a compound and its metabolites can be mapped with spatial fidelity. While previous studies have utilized computer simulations to predict molecular penetration through cells, measurement of the molecules remains the only reliable way to evaluate the effectiveness of a new species. The approach will be tested with the compounds irinotecan, oxaliplatin, and apoptotic imaging probes. This award from the Chemical Measurements and Imaging program in the Division of Chemistry is made jointly with the Instrument Development for Biological Research program in the Division of Biological Infrastructure.

在这个职业项目中，巴黎圣母院的阿曼达·霍蒙（Amanda Hummon）教授及其学生将开发一种新的质谱方法，用于分析细胞培养物中的化合物。该方法将以比其他任何方法相比，以更快，更高的通知方式提供更高的化学和空间信息。作为这项职业教育计划的一部分，Hummon教授和她的学生将通过组织每年三名当地女童子军部队的实验室巡回演出，并每学期参加两个论坛，以讨论对女性的不成比例的问题，从而制定一项针对妇女在STEM领域招募和保留的计划。该计划将鼓励年轻女学生从事科学职业。这项研究将通过质谱研究化合物将化合物渗透到三维细胞培养模型中。结合串行胰蛋白酶和纳米液相色谱 - 电喷雾串联质谱法（NLC-MS/MS），可以用空间保真度映射化合物及其代谢物的分布。尽管以前的研究已利用计算机模拟来预测通过细胞的分子穿透，但分子的测量仍然是评估新物种有效性的唯一可靠方法。该方法将用Irinotecan，Oxaliptin和凋亡成像探针进行测试。该化学划分的化学测量和成像计划的奖项与生物基础设施司的生物学研究计划共同颁发。