SBIR Phase I: A Rapid Portable Biosensor for Field Detection of Vibrio Cholerae in Environmental Water Sources

SBIR 第一阶段：用于现场检测环境水源中霍乱弧菌的快速便携式生物传感器

• 批准号: 1819970
• 负责人: Katherine Clayton
• 金额: $ 22.5万
• 依托单位: Omnivis LLC
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-07-01 至 2019-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1819970&HistoricalAwards=false
• 关键词: SBIR; Phase; Rapid; Portable; Biosensor

The broader impact/commercial potential of this Small Business Innovation Research (SBIR) project is an inexpensive handheld smartphone device for rapid detection of the toxigenic cholera pathogen in environmental water sources. Contaminated water sources place populations at risk for contracting cholera. Once contracting the disease, patients with cholera exhibit symptoms of diarrhea, vomiting, and dehydration and, if left untreated, ultimately death. Wide-scale cholera outbreaks devastated Haiti in 2010 and Yemen in 2017, affecting over one million total individuals. Currently, methods used to detect the cholera pathogen in water involves a 3 to 5-day water collection and cell culture procedure. This project proposes a portable smartphone platform used to detect the cholera pathogen, Vibrio cholerae, in under 30 minutes at the water source. Smartphone connectivity, will also enable geomapped and time-stamped detection results. This novel and proactive approach for detection can enable organizations to remediate water sources prior to communities contracting and spreading cholera. Downstream, this technology will save the time and costs currently associated with cholera outbreaks and can be expanded to other infectious diseases.This SBIR Phase I project proposes to develop a rapid, cost-effective, and robust smartphone platform to detect Vibrio cholerae and automate the detection result at an environmental water source. The device performs isothermal DNA amplification assay combined with the novel sensing approach, particle diffusometry. This project proposes to characterize the specificity, sensitivity, and lower limit of detection of Vibrio cholerae detection on the smartphone platform. The detection results will be compared against current gold-standard quantitative DNA amplification methods. Further, a reagent storage method involving freeze drying will be used to eliminate the need for cold-chain storage. We will assess the long-term stability of our assay reagents through accelerated aging studies. Lastly, a low powered integrated heating unit will be designed to perform the isothermal DNA amplification assays in the handheld device. At the completion of this Phase I project, an integrated smartphone platform will be ready for field testing.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这项小型企业创新研究（SBIR）项目的更广泛的影响/商业潜力是一种廉价的手持智能手机设备，可快速检测环境水源中的毒霍乱病原体。受污染的水源使种群有伴有霍乱的风险。一旦患病，霍乱患者就会出现腹泻，呕吐和脱水的症状，如果不加以治疗，最终死亡。大规模的霍乱疫情在2010年摧毁了海地，也门在2017年影响了超过一百万个人。当前，用于检测水中霍乱病原体的方法涉及3至5天的水收集和细胞培养程序。该项目提出了一个便携式智能手机平台，用于在水源的30分钟内检测霍乱病原体福利奥霍乱。智能手机连接性还将实现GeoMped且具有时间戳记的检测结果。这种新颖和主动的检测方法可以使组织能够在社区收缩和传播霍乱之前对水源进行补救。下游，这项技术将节省与霍乱暴发相关的时间和成本，并可以扩展到其他传染病。该SBIR I阶段项目建议开发一个快速，成本效益且强大的智能手机平台，以检测Vibrio Cholerae并在环境水源的环境水源下自动化检测结果。该设备执行等温DNA扩增测定法与新型传感方法，即颗粒扩散法。该项目建议表征智能手机平台上检测到纤维状霍乱检测的特异性，灵敏度和下限。检测结果将与当前的金标准定量DNA扩增方法进行比较。此外，将使用一种涉及冷冻干燥的试剂存储方法来消除冷链存储的需求。我们将通过加速的衰老研究评估测定试剂的长期稳定性。最后，将设计一个低动力的集成加热单元，以在手持设备中执行等温DNA扩增测定。该阶段I项目完成后，综合智能手机平台将准备好进行现场测试。该奖项反映了NSF的法定任务，并被认为是使用基金会的知识分子优点和更广泛的影响审查标准的评估值得支持的。