Collaborative Research: Study of A- and B-class dye-decolorizing peroxidases (DyPs): From molecular mechanisms to applications in dye removal and lignin degradation

合作研究：A 类和 B 类染料脱色过氧化物酶 (DyPs) 的研究：从分子机制到在染料去除和木质素降解中的应用

• 批准号: 1807532
• 负责人: Ping Li
• 金额: $ 46.18万
• 依托单位: Kansas State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-08-01 至 2023-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1807532&HistoricalAwards=false
• 关键词: Collaborative; Research; Study; class; dye

With this award, the Chemistry of Life Processes Program in the Chemistry Division is funding Dr. Ping Li from Kansas State University and Dr. Likai Song from Florida State University to investigate the molecular mechanism of A- and B-class dye-decolorizing peroxidases (DyPs) and their structure-property-reactivity-function (SPRF) relationship. The results of this investigation can be used in the development of biocatalysts for wastewater treatment and biofuel production. The proposed studies fill the critical knowledge gap regarding DyPs and guide protein engineering that could lead to enzymes with enhanced oxidative activity. This pursuit allows the graduate students and postdoctoral trainees to acquire expertise in multidisciplinary areas spanning from Chemistry to Biology, thus preparing them to become successful next-generation scientists. Contributions are also made to an outreach program that encourages students from groups underrepresented in STEM careers to major in science. This research project seeks to elucidate the molecular mechanism and SPRF relationship of DyPs, a new family of H2O2-dependent heme peroxidases. The studies focus on TcDyP from Thermomonospora curvata and ElDyP from Enterobacter lignolyticus as the representatives for the A- and B-class enzymes from this family, respectively. The residues situated on the enzyme surface that are responsible for substrate oxidation in TcDyP, the residue(s) that control one- and two-electron reduction reactions that involve ElDyP compound I, and the dynamics of the loop surrounding the heme pocket in DyPs are being studied using structural biology, enzymology, and electron paramagnetic resonance (EPR) investigation methods. Organic synthesis and protein engineering are being utilized to define and modulate the SPRF relationship. Information from this study not only fills the fundamental knowledge gap on DyPs but also paves the way for DyPs' applications in dye removal and lignin degradation.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

有了这个奖项，化学过程的化学过程计划是为堪萨斯州立大学的Ping Li博士和佛罗里达州立大学的Likai Song提供资金，以研究A-和B级染料和B级染料染色过氧化物酶（DYPS）的分子机制及其结构 - 特性 - 特性 - 反应性 - 反应性官能功能（SPRF）的关系。这项研究的结果可用于废水处理和生物燃料生产的生物催化剂的开发。拟议的研究填补了有关DYPS和引导蛋白质工程的关键知识差距，这些差距可能导致具有增强氧化活性的酶。这种追求使研究生和博士后学员可以在从化学到生物学的多学科领域获得专业知识，从而使他们成为成功的下一代科学家。还为一项宣传计划做出了贡献，该计划鼓励来自STEM职业人数不足的小组的学生对科学专业的专业。 该研究项目旨在阐明DYPS的分子机制和SPRF关系，DYP是H2O2依赖性血红素过氧化物酶的新家族。这些研究重点介绍了来自肠杆菌木洛氏菌群的TCDYP和ELDYP，分别是该家族的A-和B级酶的代表。位于酶表面上的残基，负责TCDYP中的底物氧化，控制涉及ELDYP化合物I的单一和两电子还原反应的残基，以及使用结构生物学，inzymologation（Electron paramagice e re Resenance（Electron promanane）进行研究，涉及ELDYP化合物I，以及围绕DYP的循环的动力学。有机合成和蛋白质工程被用于定义和调节SPRF关系。这项研究的信息不仅填补了DYP的基本知识差距，而且还为DYPS在去除染料和木质素降解方面的应用铺平了道路。该奖项反映了NSF的法定任务，并被认为是值得通过基金会的知识分子优点和更广泛的影响审查标准来通过评估来通过评估来支持的。

项目成果

Mechanistic Insights into DyPB from Rhodococcus jostii RHA1 Via Kinetic Characterization

通过动力学表征从红球菌 jostii RHA1 中了解 DyPB 的机制

• DOI: 10.1021/acscatal.1c00703
• 发表时间: 2021
• 期刊: ACS Catalysis
• 影响因子: 12.9
• 作者: Shrestha, Ruben;Jia, Kaimin;Khadka, Samiksha;Eltis, Lindsay D.;Li, Ping
• 通讯作者: Li, Ping