Collaborative Research: Teasing apart how specific nanoparticle features relate to environmental fate and contribute to ecotoxicity

合作研究：梳理特定纳米颗粒特征如何与环境命运相关并导致生态毒性

• 批准号: 1762245
• 负责人: Stacey Harper
• 金额: $ 28.78万
• 依托单位: Oregon State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-04-15 至 2021-03-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1762245&HistoricalAwards=false
• 关键词: Collaborative; Research; Teasing; apart; how; Collaborative; Research; Teasing; apart; how

Silver nanoparticles are extensively used for their antimicrobial properties in an increasing number of consumer and commercial products and as bacterial agents in the treatment of wastewater. Because of their high demand, over 500 tons of engineered silver nanoparticles are produced globally. While the properties of these nanoparticles are important for their antibacterial activity, some may also influence toxicity. The environmental impacts of silver nanoparticles independent of their ion leaching are challenging at best. Studies designed to evaluate the nanoparticle-specific effects of silver nanoparticles have been limited because of their propensity to undergo ionic dissolution. Furthermore, discrepancies over the relative contribution of ion to particle toxicity remain prevalent in the literature primarily due to differences in study design. Consequently, there is a great need to understand how silver nanoparticles with diverse characteristics impacts the interaction with organisms by controlling for the confounding effects of ionic contribution to toxicity as compared to particle effects. The researcher's proposed studies will contribute to the scientific understanding of how the properties of these nanoparticles play a role in biouptake, nanoparticle-biological interactions, and ecotoxicity. The research will involve the elimination of the effects of particle surface oxidation and ionic dissolution, which has complicated toxicity studies in the past. This contribution is significant because it will improve our ability to identify features of silver nanoparticles that make them eco-disruptive and predict how these features lead to adverse environmental outcomes. All data will be shared through the open-source knowledge base of Nanomaterial-Biological Interactions (NBI) globally for modeling efforts and will support the development of safety protocols, exposure guidelines, and regulations that protect human and ecosystem health. Furthermore, this research will provide design rules for the assembly of new classes of silver nanoparticles that could be commercialized without concern regarding rapid particle degradation and release into the environment. In addition, this project is designed to incorporate students from diverse backgrounds and will help build future science, technology, engineering and math (STEM) talent. The researcher's overall aim is to improve our understanding of the specific physiochemical features that dictate nanoparticle-biological interactions. First, they will design a series of lipid-coated silver nanoparticles that are differentially shielded from ion dissolution. Differentially shielded silver nanoparticles will be prepared by encapsulating silver nanoparticles of varying size and shape with a hybrid lipid-membrane to protect the surface from oxidation and ionic dissolution. Changes in the localized surface plasmon resonance (LSPR), thermal electron microscope (TEM), and (Inductively-coupled plasma mass spectrometer (ICP-MS) will be employed to monitor silver ion dissolution from the suite of nanoparticles. Second, they will identify features of lipid-coated nanoparticles that lead to particle instability. The agglomeration kinetics of the hybrid lipid-coated silver nanoparticles will be assessed using dynamic light scattering and nanoparticle tracking analysis. Third, since the goal is to ultimately relate these material features with nanoparticle-biological interactions, the researchers will determine the uptake and toxicity of the silver nanoparticle suite. Based on preliminary investigations, the hybrid lipid-coated silver nanoparticles with a robust coating should elicit minimal toxicity and a decrease in surface coverage should lead to a respective increase in toxicity. A well-established embryonic zebrafish assay will be used to identify vertebrate morbidity and mortality resulting from exposure and hyperspectral imaging (HSI) will be used to visualize nanoparticle uptake in whole animals. Finally, the researchers will assess the potential ecotoxicity of the suite using a novel nanocosm assay. Hyperspectral imaging will be used to visualize nanoparticle biodistribution among bacteria, algae, crustaceans, and fish in the small-scale freshwater assay. Collectively, the use of well-characterized silver nanoparticles tuned for ion release will allow the PIs to tease apart the relative contribution of the nanoparticle and ion to biouptake, toxicity, and potential for environmental impacts.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

银纳米颗粒在越来越多的消费者和商业产品中广泛用于其抗菌特性，作为废水处理的细菌剂。由于需求量很高，全球生产超过500吨工程的银纳米颗粒。尽管这些纳米颗粒的特性对于其抗菌活性很重要，但有些也可能影响毒性。银纳米颗粒与离子浸出无关的环境影响充其量是具有挑战性的。旨在评估银纳米颗粒的纳米颗粒特异性作用的研究受到限制，因为它们倾向于经历离子溶解。此外，在文献中，离子对颗粒毒性相对贡献的相对贡献的差异主要是由于研究设计的差异。因此，非常需要了解具有不同特征的银纳米颗粒如何通过控制离子对毒性的混杂作用而与生物体相互作用，而与颗粒效应相比。研究人员的拟议研究将有助于对这些纳米颗粒的性质如何在生物群，纳米粒子生物学相互作用和生态毒性中发挥作用的科学理解。这项研究将涉及消除颗粒表面氧化和离子溶解的影响，这在过去曾复杂化毒性研究。这项贡献很重要，因为它将提高我们识别银纳米颗粒的特征的能力，这些特征使它们具有生态破坏性，并预测这些特征如何导致不利的环境结果。所有数据将通过全球纳米材料生物相互作用（NBI）的开源知识基础进行建模，并将支持保护人类和生态系统健康的安全协议，暴露准则和法规的制定。此外，这项研究将为组装新类的银纳米颗粒提供设计规则，这些规则可以商业化，而不必担心快速粒子降解并释放到环境中。此外，该项目旨在融合来自不同背景的学生，并将有助于建立未来的科学，技术，工程和数学（STEM）人才。研究人员的总体目的是提高我们对决定纳米粒子生物相互作用的特定生理化学特征的理解。首先，他们将设计一系列脂质涂层的银纳米颗粒，这些银纳米颗粒被差异化，以免受离子溶解的影响。通过将带有变化的银纳米颗粒与杂化脂质膜形状封装，以保护表面免受氧化和离子溶解的态度来制备差异屏蔽的银纳米颗粒。将使用局部表面等离激子共振（LSPR），热电子显微镜（TEM）和（（将使用电感耦合等离子体质谱仪（ICP-MS））监测从纳米颗粒的套件中溶解的银离子溶解。其次，它们将识别出脂质含量的纳米粒子的特征，使粒子构成的特征构成了杂物的构成kin，kinbr kin to aggid kin kin kin kin kin kin the the aggid kin the the aggid。银纳米颗粒将使用动态光散射和纳米粒子跟踪分析进行评估，因为该目标是将这些材料特征与纳米颗粒 - 生物相互作用联系起来，研究人员将基于初步的nanopility the imim coalim a nanopity a imim to ripid coaltions a nanopirations a nanopirations sillim a nanopity，表面覆盖范围的降低应导致毒性各自增加。最后，研究人员将使用新型的纳米测定法评估套件的潜在生态毒性。高光谱成像将用于可视化细菌，藻类，甲壳类动物和小型淡水分析中的纳米颗粒生物分布。总的来说，使用用于离子释放的良好特征化的银纳米颗粒将使PI嘲笑纳米颗粒和离子对生物蛋白能，毒性以及对环境影响的潜在影响的相对贡献。这奖反映了NSF的法定任务，并通过使用基金会的智力效果和宽阔的评估来进行评估，并以评估值得评估。