Collaborative Research: Teasing apart how specific nanoparticle features relate to environmental fate and contribute to ecotoxicity

合作研究：梳理特定纳米颗粒特征如何与环境命运相关并导致生态毒性

• 批准号: 1762278
• 负责人: Marilyn Mackiewicz
• 金额: $ 24.46万
• 依托单位: Portland State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-04-15 至 2021-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1762278&HistoricalAwards=false
• 关键词: Collaborative; Research; Teasing; apart; how

Silver nanoparticles are extensively used for their antimicrobial properties in an increasing number of consumer and commercial products as well as in wastewater treatment. Understanding the potential toxicity and environmental impacts of silver nanoparticles is challenging. Because silver nanoparticles undergo ionic dissolution, discrepancies exist over the relative contribution of silver ions or the nanoparticles themselves in toxicity. This project will increase understanding of how silver nanoparticle properties affect bio-uptake, nanoparticle-biological interactions, and ecotoxicity. Effects of particle surface oxidation and ionic dissolution, which have complicated toxicity studies in the past, will be eliminated. This study will identify properties of silver nanoparticles that make them ecologically disruptive leading to adverse environmental outcomes. All data will be shared through the open-source knowledge base of Nanomaterial-Biological Interactions (NBI) globally for modeling efforts and will support the development of safety protocols, exposure guidelines, and regulations that protect human and ecosystem health. Furthermore, this research will provide design rules for the assembly of new classes of silver nanoparticles that could be commercialized without concern regarding rapid particle degradation and release into the environment. In addition, this project is designed to incorporate students from diverse backgrounds and will help build future science, technology, engineering and math (STEM) talent.The researcher's overall aim is to improve our understanding of the specific physiochemical features that dictate nanoparticle-biological interactions. First, they will design a series of lipid-coated silver nanoparticles that are differentially shielded from ion dissolution. Differentially shielded silver nanoparticles will be prepared by encapsulating silver nanoparticles of varying size and shape with a hybrid lipid-membrane to protect the surface from oxidation and ionic dissolution. Changes in the localized surface plasmon resonance (LSPR), thermal electron microscope (TEM), and (Inductively-coupled plasma mass spectrometer (ICP-MS) will be employed to monitor silver ion dissolution from the suite of nanoparticles. Second, they will identify features of lipid-coated nanoparticles that lead to particle instability. The agglomeration kinetics of the hybrid lipid-coated silver nanoparticles will be assessed using dynamic light scattering and nanoparticle tracking analysis. Third, since the goal is to ultimately relate these material features with nanoparticle-biological interactions, the researchers will determine the uptake and toxicity of the silver nanoparticle suite. Based on preliminary investigations, the hybrid lipid-coated silver nanoparticles with a robust coating should elicit minimal toxicity and a decrease in surface coverage should lead to a respective increase in toxicity. A well-established embryonic zebrafish assay will be used to identify vertebrate morbidity and mortality resulting from exposure and hyperspectral imaging (HSI) will be used to visualize nanoparticle uptake in whole animals. Finally, the researchers will assess the potential ecotoxicity of the suite using a novel nanocosm assay. Hyperspectral imaging will be used to visualize nanoparticle biodistribution among bacteria, algae, crustaceans, and fish in the small-scale freshwater assay. Collectively, the use of well-characterized silver nanoparticles tuned for ion release will allow the PIs to tease apart the relative contribution of the nanoparticle and ion to biouptake, toxicity, and potential for environmental impacts.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

银纳米颗粒在越来越多的消费者和商业产品以及废水处理中广泛用于其抗菌特性。 了解银纳米颗粒的潜在毒性和环境影响是具有挑战性的。由于银纳米颗粒会经历离子溶解，因此银离子或纳米颗粒本身的相对贡献存在差异。 该项目将增加对银纳米颗粒特性如何影响生物摄取，纳米颗粒生物相互作用和生态毒性的理解。 过去将消除具有复杂毒性研究的颗粒表面氧化和离子溶解的影响。这项研究将确定银纳米颗粒的特性，使它们在生态破坏性上导致不利的环境结果。所有数据将通过全球纳米材料生物相互作用（NBI）的开源知识基础进行建模，并将支持保护人类和生态系统健康的安全协议，暴露准则和法规的制定。此外，这项研究将为组装新类的银纳米颗粒提供设计规则，这些规则可以商业化，而不必担心快速粒子降解并释放到环境中。此外，该项目旨在融合来自不同背景的学生，并将有助于建立未来的科学，技术，工程和数学（STEM）才华。研究人员的总体目的是提高我们对决定纳米粒子生物互动的特定生理学特征的理解。首先，他们将设计一系列脂质涂层的银纳米颗粒，这些银纳米颗粒被差异化，以免受离子溶解的影响。通过将带有变化的银纳米颗粒与杂化脂质膜形状封装，以保护表面免受氧化和离子溶解的态度来制备差异屏蔽的银纳米颗粒。将使用局部表面等离激子共振（LSPR），热电子显微镜（TEM）和（（将使用电感耦合等离子体质谱仪（ICP-MS））监测从纳米颗粒的套件中溶解的银离子溶解。其次，它们将识别出脂质含量的纳米粒子的特征，使粒子构成的特征构成了杂物的构成kin，kinbr kin to aggid kin kin kin kin kin kin the the aggid kin the the aggid。银纳米颗粒将使用动态光散射和纳米粒子跟踪分析进行评估，因为该目标是将这些材料特征与纳米颗粒 - 生物相互作用联系起来，研究人员将基于初步的nanopility the imim coalim a nanopity a imim to ripid coaltions a nanopirations a nanopirations sillim a nanopity，表面覆盖范围的降低应导致毒性各自增加。最后，研究人员将使用新型的纳米测定法评估套件的潜在生态毒性。高光谱成像将用于可视化细菌，藻类，甲壳类动物和小型淡水分析中的纳米颗粒生物分布。总的来说，使用用于离子释放的良好特征化的银纳米颗粒将使PI嘲笑纳米颗粒和离子对生物蛋白能，毒性以及对环境影响的潜在影响的相对贡献。这奖反映了NSF的法定任务，并通过使用基金会的智力效果和宽阔的评估来进行评估，并以评估值得评估。