MRI: Acquisition of a High Resolution GC-MS-MS with Fully Automated Sample Preparation to Advance Chemical Synthesis, Metabolomics, and Geochemical Research at IU Bloomington

MRI：购买高分辨率 GC-MS-MS 和全自动样品制备，以推进印第安纳大学伯明顿分校的化学合成、代谢组学和地球化学研究

• 批准号: 1726633
• 负责人: Jonathan Karty
• 金额: $ 43.75万
• 依托单位: Indiana University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1726633&HistoricalAwards=false
• 关键词: MRI; Acquisition; Resolution; GC; MS

This award is supported by the Major Research Instrumentation (MRI) and the Chemistry Research Instrumentation (CRIF) programs. Professor Jonathan Karty from Indiana University and colleagues Simon Brassell, Silas Cook, James McKinlay and Jason Tennessen are acquiring a high resolution gas-chromatograph tandem mass spectrometer with tandem capabilities (HRes GC-MS-MS.) In general, mass spectrometry (MS) is one of the key analytical methods used to identify and characterize small quantities of chemical species in complex matrices. In a typical experiment, the components go into a mass spectrometer where they are ionized and the ion masses are measured. This highly sensitive technique allows detection and determination of the structure of molecules in a complex mixture. An instrument with a gas chromatograph provides additional structural identification power by separating gaseous mixtures of compounds before they reach the mass spectrometer. The tandem capabilities allow for additional separation in a second stage. This acquisition strengthens the research infrastructure at the University. The instrument broadens participation by involving diverse students in research and training using this modern analytical technique. It also provides training opportunities to many undergraduate, graduate and postdoctoral students from chemical, biological, geological and medicinal sciences as well as high school students through a variety of programs such as the Indiana University Summer Scholars Institute for students at minority serving institutions as well as the Louis Stokes Alliance for Minority Participation.This mass spectrometer enhances research and education at all levels. It enables research that elucidates of the role of climate on human evolution by measuring paleoclimate markers from Olduvai Gorge and explores the metabolic basis for cell proliferation and growth in Drosophila. The instrumentation aids in carrying out 13-carbon experiments to quantify metabolic pathway utilization in microbes as well as in distinguishing intermediates generated during organic syntheses of natural products. The mass spectrometer is also used in profiling and characterizing pheromones and sustainable carbon-carbon bond forming catalysts.

该奖项由主要研究仪器（MRI）和化学研究仪器（CRIF）项目支持。印第安纳大学的 Jonathan Karty 教授及其同事 Simon Brassell、Silas Cook、James McKinlay 和 Jason Tennessen 正在购买一台具有串联功能的高分辨率气相色谱串联质谱仪 (HRes GC-MS-MS)。是用于识别和表征复杂基质中少量化学物质的关键分析方法之一。在典型的实验中，组分进入质谱仪，在那里它们被电离并测量离子质量。这种高度灵敏的技术可以检测和确定复杂混合物中的分子结构。带有气相色谱仪的仪器通过在化合物的气态混合物到达质谱仪之前对其进行分离来提供额外的结构识别能力。串联功能允许在第二阶段进行额外的分离。此次收购加强了大学的研究基础设施。该仪器通过让不同的学生参与使用这种现代分析技术的研究和培训来扩大参与范围。它还通过印第安纳大学暑期学者学院等各种项目，为化学、生物、地质和医学科学领域的许多本科生、研究生和博士后学生以及高中生提供培训机会。路易斯·斯托克斯少数民族参与联盟。这款质谱仪增强了各个层面的研究和教育。它可以通过测量奥杜瓦伊峡谷的古气候标记来阐明气候对人类进化的作用，并探索果蝇细胞增殖和生长的代谢基础。该仪器有助于进行 13 碳实验，以量化微生物代谢途径的利用，以及区分天然产物有机合成过程中产生的中间体。该质谱仪还用于分析和表征信息素和可持续的碳-碳键形成催化剂。

项目成果

Copper-Catalyzed, N-Directed Csp 3 –H Trifluoromethylthiolation (−SCF 3 ) and Trifluoromethylselenation (−SeCF 3 )

铜催化的 N 定向 Csp 3 →H 三氟甲硫基化 (–SCF 3 ) 和三氟甲基硒化 (–SeCF 3 )

• DOI: 10.1021/jacs.9b10316
• 发表时间: 2019-10
• 期刊: Journal of the American Chemical Society
• 影响因子: 15
• 作者: Modak, Atanu;Pinter, Emily N.;Cook, Silas P.
• 通讯作者: Cook, Silas P.

The oncometabolite L-2-hydroxyglutarate is a common product of dipteran larval development

致癌代谢物 L-2-羟基戊二酸是双翅目幼虫发育的常见产物

• DOI: 10.1016/j.ibmb.2020.103493
• 发表时间: 2020-12
• 期刊: Insect Biochemistry and Molecular Biology
• 影响因子: 3.8
• 作者: Mahmoudzadeh NH;Fitt AJ;Schwab DB;Martenis WE;Nease LM;Owings CG;Brinkley GJ;Li H;Karty JA;Sudarshan S;Hardy RW;Moczek AP;Picard CJ;Tennessen JM
• 通讯作者: Tennessen JM

Modular Synthesis of a Versatile Double‐Allylation Reagent for Complex Diol Synthesis

用于复杂二醇合成的多功能双烯丙基化试剂的模块化合成

• DOI: 10.1002/anie.202103435
• 发表时间: 2021-06
• 期刊: Angewandte Chemie International Edition
• 作者: Dorn, Stanna K.;Tharp, Annika E.;Brown, M. Kevin
• 通讯作者: Brown, M. Kevin

Lessons in Strain and Stability: Enantioselective Synthesis of (+)‐[5]‐Ladderanoic Acid

应变和稳定性的经验教训：( )→[5]→梯烷酸的对映选择性合成

• DOI: 10.1002/anie.201910901
• 发表时间: 2019-11
• 期刊: Angewandte Chemie International Edition
• 作者: Hancock, Erin N.;Kuker, Erin L.;Tantillo, Dean J.;Brown, M. Kevin
• 通讯作者: Brown, M. Kevin

Biogeochemical evidence for environmental changes of Pleistocene Lake Olduvai during the transitional sequence of OGCP core 2A that encompasses Tuff IB (~1.848 Ma)

包含凝灰岩 IB (~1.848–Ma) 的 OGCP 核心 2A 过渡序列期间更新世奥杜威湖环境变化的生物地球化学证据

• DOI: 10.1016/j.palaeo.2019.109267
• 发表时间: 2019-10
• 期刊: Palaeoecology
• 作者: Shilling, Andrea M.;Colcord, Devon E.;Karty, Jonathan;Hansen, Angela;Freeman, Katherine H.;Njau, Jackson K.;Stanistreet, Ian G.;Stollhofen, Harald;Schick, Kathy D.;Toth, Nicholas;et al
• 通讯作者: et al