RUI: Mechanistic study of short N-acetylglutamate synthetases and incorporation into a course-based undergraduate research experience

RUI：短N-乙酰谷氨酸合成酶的机理研究并纳入基于课程的本科生研究经验

• 批准号: 1708828
• 负责人: Emily Fogle
• 金额: $ 13.73万
• 依托单位: California Polytechnic State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-08-15 至 2022-12-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1708828&HistoricalAwards=false
• 关键词: RUI; Mechanistic; study; short; acetylglutamate

The Chemistry of Life Processes Program in the Chemistry Division funds this work. Professors Emily Fogle and Eric Jones from California Polytechnic State University, San Luis Obispo (Cal Poly) study enzyme catalyzed reactions of compounds containing sulfur, thioesters. Thioesters are a class of reactive molecules with important roles in both chemistry and biochemistry. Thioesters are common intermediates in many biosynthetic reactions, including starting materials for steroid production. The research examines enzymatic reactions of thioesters with nitrogen-containing molecules and to studies of a common bacterial form of the enzyme NAGS (N-acetylglutamate synthase). This research is incorporated into a self-sustaining research experience that occurs in an upper division undergraduate laboratory class. The work is incorporated into a required, advanced biochemistry course that biochemistry majors take in their junior year. The goal is for all biochemistry majors at Cal Poly to have an authentic research experience and gain knowledge, skills and self-confidence. The research project studies the enzymatic reaction of thioesters with nitrogen nucleophiles (aminolysis reactions) and characterizes a common bacterial form of N-acetylglutamate synthase (NAGS). Thioesters are a very important class of reactive carboxylic acid derivatives in which a sulfur atom replaces the typical oxygen or nitrogen atom in the leaving group position. Thioesters are generally more highly reactive toward nucleophiles than the equivalent oxoesters and this factor has resulted in important roles in both organic chemistry and biochemistry. The specific scientific aims of this work are to purify and kinetically characterize three bacterial NAGS enzymes and to determine the nitrogen and sulfur kinetic isotope effects for the NAGS-catalyzed reaction. This work is also incorporated into a self-sustaining research experience that occurs in an upper division laboratory class.

化学部门的化学过程计划为这项工作提供了资金。来自加利福尼亚理工大学的艾米丽·福格尔（Emily Fogle）和埃里克·琼斯（Eric Jones），圣路易斯·奥比斯波（Cal Poly）研究酶催化含有硫的化合物的反应，含硫，硫植物。硫代植物是一类反应性分子，在化学和生物化学中都具有重要作用。硫代植物是许多生物合成反应的常见中间体，包括用于类固醇的起始材料。 该研究研究了硫硫代硫代分子的酶促反应，并研究了酶NAGS（N-乙酰基谷氨酸合酶）的常见细菌形式的研究。 这项研究纳入了上层本科实验室班级中发生的自我维持的研究经验。这项工作纳入了生物化学专业大三的必要的高级生物化学课程中。目的是让Cal Poly的所有生物化学专业都具有真实的研究经验并获得知识，技能和自信心。该研究项目研究了硫代硫代核菌（氨基溶解反应）的酶促反应，并表征了N-乙酰基谷氨酸合酶（NAGS）的常见细菌形式。硫代植物是一类非常重要的反应性羧酸衍生物，其中硫原子在离开组的位置取代了典型的氧气或氮原子。硫代植物通常比等效的氧化剂对亲核试剂的反应性高，并且该因素在有机化学和生物化学中既有重要作用。这项工作的具体科学目的是纯化和动力学表征三种细菌nags酶，并确定氮和硫动力学同位素效应，以使NAGS催化反应。这项工作还纳入了上层实验室类别中发生的自我维持的研究经验。

项目成果

A report on learning gains and student confidence after incorporating an enzyme purification and characterization course‐based undergraduate experience

关于纳入基于酶纯化和表征课程的本科经历后的学习收获和学生信心的报告

• DOI: 10.1002/bmb.21698
• 发表时间: 2022
• 期刊: Biochemistry and Molecular Biology Education
• 影响因子: 1.4
• 作者: Jones, Eric;Glanz, Hunter;Wilkinson, Steven;Fogle, Emily
• 通讯作者: Fogle, Emily