RUI: Development of novel surface-modified cellulose nanocrystals (CNCs) for biomedical application as vaccine adjuvants: a descriptive study

RUI：开发新型表面改性纤维素纳米晶体（CNC）作为疫苗佐剂用于生物医学应用：描述性研究

• 批准号: 1703890
• 负责人: Karina Ckless
• 金额: $ 33万
• 依托单位: SUNY College at Plattsburgh
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2017
• 资助国家: 美国
• 起止时间: 2017-09-01 至 2022-02-28
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1703890&HistoricalAwards=false
• 关键词: RUI; Development; novel; surface; modified

Cellulose nanocrystals have emerged as a new class of renewable materials for various applications due to their remarkable properties and commercialization prospects. Cellulose nanocrystals are also being explored for biomedical applications, including drug carriers and vaccine adjuvants. Vaccine adjuvants are substances or molecules that activate our immunological system, causing the vaccines to be more effective. For example, aluminum hydroxide, a particulate inorganic material, is the most commonly used vaccine adjuvant, because it has a record of safety and efficacy. However, in some types of vaccines, aluminum hydroxide is not always the most effective choice. Therefore, developing new organic nanomaterials with potential vaccine adjuvant properties will broaden the available number of systems so they can be selected to give the best results for a given vaccine. This research project involves designing, developing, and characterizing a range of cellulose nanocrystals and then determining their safety and immune responses. Additionally, this research project is impacting undergraduate student education, since they will be directly involved in original research activities. Students are being trained on experimental planning and data collection, as well as data interpretation and research presentation skills. The experiential learning provided through this research project would make SUNY-Plattsburgh students better prepared when seeking employment or graduate school opportunities following graduation. In this project, the researchers will develop a library of modified cationic cellulose nanocrystals, by adding positively charged polymers to their surfaces. These modified cellulose nanocrystals will be characterized using spectroscopic, analytical and microscopic techniques. The toxicity and potential immunogenicity of these nanoparticles will be assessed using mammalian cells as a biological tool. The data generated form cell culture studies will provide a solid basis for future animal studies to develop further vaccine adjuvants. Also, these in vitro studies can form a foundation for evaluating the in vivo safety and biological activities of new biomedical cellulose-based nanomaterials. Undergraduate students are being thoroughly integrated into all aspects of the research, from synthesis and characterization of modified cationic cellulose nanocrystals to cell culture techniques and immunogenicity and toxicity assays, as well as gene expression, protein isolation and identification methods.

纤维素纳米晶体由于其出色的特性和商业化前景而成为各种应用的新型可再生材料。 还在用于生物医学应用，包括药物载体和疫苗佐剂的生物医学应用中探索纤维素纳米晶体。疫苗佐剂是激活我们的免疫系统的物质或分子，导致疫苗更有效。例如，氢氧化铝（一种无机材料）是最常用的疫苗佐剂，因为它具有安全性和有效性的记录。但是，在某些类型的疫苗中，氢氧化铝并不总是最有效的选择。因此，开发具有潜在疫苗辅助性能的新的有机纳米材料将扩大可用的系统数量，因此可以选择它们以给定疫苗提供最佳效果。 该研究项目涉及设计，开发和表征一系列纤维素纳米晶体，然后确定其安全性和免疫反应。此外，该研究项目正在影响学生教育，因为它们将直接参与原始研究活动。学生正在接受实验计划和数据收集以及数据解释和研究演示技巧的培训。通过该研究项目提供的体验式学习将使Suny-Plattsburgh学生在毕业后寻求就业或研究生院机会时做好准备。在这个项目中，研究人员将通过在其表面上添加带正电荷的聚合物来开发一个修改的阳离子纤维素纳米晶体库。这些修饰的纤维素纳米晶将使用光谱，分析和显微镜技术来表征。这些纳米颗粒的毒性和潜在免疫原性将使用哺乳动物细胞作为生物学工具进行评估。生成的细胞培养研究生成的数据将为未来的动物研究提供良好的基础，以开发进一步的疫苗佐剂。此外，这些体外研究可以为评估新的基于生物医学纤维素的纳米材料的体内安全性和生物学活性构成基础。从修饰的阳离子纤维素纳米晶体的合成和表征到细胞培养技术以及免疫原性和毒性测定，以及基因表达，蛋白质分离和鉴定方法，本科生都被彻底整合到研究的各个方面。

项目成果

Mechanisms of the immune response cause by cationic and anionic surface functionalized cellulose nanocrystals using cell-based assays

• DOI: 10.1016/j.tiv.2018.12.009
• 发表时间: 2019-03-01
• 期刊: TOXICOLOGY IN VITRO
• 影响因子: 3.2
• 作者: Despres, Hannah W.;Sabra, Adham;Ckless, Karina
• 通讯作者: Ckless, Karina

Cell-based analysis of the immune and antioxidant response of the nanocarrier β-cyclodextrin conjugated with cellulose nanocrystals

基于细胞的纳米载体β-环糊精与纤维素纳米晶体缀合的免疫和抗氧化反应分析

• DOI: 10.1016/j.freeradbiomed.2018.10.244
• 发表时间: 2018
• 期刊: Free Radical Biology and Medicine
• 影响因子: 7.4
• 作者: Despres, Hannah;Sunasee, Rajesh;Carson, Melissa;Pacherille, Angela;Nunez, Kaylee;Ckless, Karina
• 通讯作者: Ckless, Karina

Analysis of the Immune and Antioxidant Response of Cellulose Nanocrystals Grafted with β -Cyclodextrin in Myeloid Cell Lines

骨髓细胞系中接枝β-环糊精的纤维素纳米晶体的免疫和抗氧化反应分析

• DOI: 10.1155/2019/4751827
• 发表时间: 2019
• 期刊: Journal of Nanomaterials
• 作者: Sunasee, Rajesh;Carson, Melissa;Despres, Hannah W.;Pacherille, Angela;Nunez, Kaylee D.;Ckless, Karina
• 通讯作者: Ckless, Karina

Synthesis and Cytotoxicity Studies of Wood-Based Cationic Cellulose Nanocrystals as Potential Immunomodulators

• DOI: 10.3390/nano10081603
• 发表时间: 2020-08-01
• 期刊: NANOMATERIALS
• 影响因子: 5.3
• 作者: Imtiaz, Yusha;Tuga, Beza;Sunasee, Rajesh
• 通讯作者: Sunasee, Rajesh