EAGER: Non-invasive Sensing of Superficial Organ Tissue via Conforming Multi-parametric Microfluidic Organ Biosensors (MMOBs): Shifting the Paradigm for Organ Assessment

EAGER：通过多参数微流控器官生物传感器 (MMOB) 对浅表器官组织进行非侵入式传感：改变器官评估的范式

• 批准号: 1650601
• 负责人: Blake Johnson
• 金额: $ 15万
• 依托单位: Virginia Polytechnic Institute and State University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-09-01 至 2019-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1650601&HistoricalAwards=false
• 关键词: EAGER; Non; invasive; Sensing; Superficial

PI: Johnson, Blake Proposal Number: 1650601 Organs for transplantation, such as kidneys, are in short supply. Good measures that predict the health status of organs targeted for transplantation are not available. The current approach is not objective. This project will use current state of the art methods to make devices that will be attached to live kidneys in a special apparatus for obtaining important measurements using sensors that will be fabricated as part of the project. The goal is that such measurements will take the guess work out of the health assessment of the organ transplantation procedure. The goal of the proposed project is to fabricate a conforming and flexible sensor that will be adaptive to ex vivo organ surfaces (the porcine kidney in the experiments) for obtaining electrical impedance data, and measure biomarkers in perfused fluids using a microfluidic arrangement. The project will use advanced fabrication approach of 3D structured light scanning and extrusion-based additive manufacturing methodology for fabricating conformal (form-fitting) microfluidic organ biosensors. This sensor-based identification of biomarkers and impedance signatures in superficial tissue matrix is expected to correlate with organ health. The project will provide an educational opportunity by creating a two-week workshop which will train students in biomedical device manufacturing. Educational benefits will be assessed through a faculty-judged BIO-Maker fair.

PI：Johnson、Blake 提案编号：1650601 用于移植的器官，例如肾脏，供应短缺。 目前还没有预测移植目标器官健康状况的良好措施。目前的做法并不客观。 该项目将使用当前最先进的方法来制造装置，这些装置将连接到特殊装置中的活体肾脏上，以便使用将作为该项目的一部分制造的传感器来获得重要的测量结果。 目标是此类测量将消除器官移植程序健康评估中的猜测工作。该项目的目标是制造一种顺应且灵活的传感器，该传感器将适应离体器官表面（实验中的猪肾）以获得电阻抗数据，并使用微流体布置测量灌注液中的生物标志物。该项目将使用 3D 结构光扫描的先进制造方法和基于挤压的增材制造方法来制造保形（形状配合）微流体器官生物传感器。 这种基于传感器的浅表组织基质中生物标志物和阻抗特征的识别预计将与器官健康相关。该项目将通过举办为期两周的研讨会来提供教育机会，对学生进行生物医学设备制造方面的培训。教育效益将通过由教师评审的 BIO-Maker 博览会进行评估。

项目成果

A Hybrid 3D Printing and Robotic-assisted Embedding Approach for Design and Fabrication of Nerve Cuffs with Integrated Locking Mechanisms

用于设计和制造具有集成锁定机构的神经袖带的混合 3D 打印和机器人辅助嵌入方法

• DOI: 10.1557/adv.2018.378
• 发表时间: 2018
• 期刊: MRS Advances
• 影响因子: 0.8
• 作者: Tong, Yuxin;Murbach, Jamie M.;Subramanian, Vivek;Chhatre, Shrirang;Delgado, Francisco;Martin, David C.;Otto, Kevin J.;Romero-Ortega, Mario;Johnson, Blake N.
• 通讯作者: Johnson, Blake N.