RAPID: Determining the extrinsic incubation period and transovarial transmission potential of Zika virus in Aedes aegypti mosquitoes

RAPID：确定寨卡病毒在埃及伊蚊中的外在潜伏期和跨卵巢传播潜力

• 批准号: 1641029
• 负责人: Michael Riehle
• 金额: $ 19.82万
• 依托单位: University of Arizona
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1641029&HistoricalAwards=false
• 关键词: RAPID; Determining; extrinsic; incubation; period

This RAPID project will determine how temperature affects the time it takes from when a mosquito takes up Zika virus from one person until it can transmit the virus to another person. It will also test for potential transmission of the virus from mosquito mothers to their offspring. This information is necessary to accurately predict the timing and geographic spread of the disease in South, Central and North America. Zika virus threatens the safety of people in the United States, and around the globe. Key information generated by this study will help construct accurate predictive models and will identify areas of high risk in need of public health prioritization. Results from this project will be relevant to the Zika public health emergency, and the researchers have set in place mechanisms to share quality-assured interim and final data as rapidly and widely as possible, including with public health and research communities.Two critical gaps of knowledge concerning Zika virus and its interactions with Aedes aegypti are its extrinsic incubation period (EIP) and the virus potential to be transmitted from a mosquito to its offspring (transovarial or vertical transmission potential). The EIP is the length of time from when a mosquito is first exposed to the virus, to when the virus has migrated to the salivary gland and the mosquito is capable of spreading it at its next feeding. The EIP and potential for vertical transmission of Zika virus in mosquito vectors is critical to accurately modeling the spread of the virus across the continent. The team will expose the mosquitos to a range of viral concentrations at difference temperatures, and will utilize genetic tools to quantify the virus and characterize the timing of spread within the mosquito. Furthermore the research team will explore how these variables are affected by co-infection with dengue virus.

该 RAPID 项目将确定温度如何影响从蚊子从一个人身上吸收寨卡病毒到将病毒传播给另一个人所需的时间。它还将测试病毒从蚊子母亲传播给后代的可能性。这些信息对于准确预测该疾病在南美洲、中美洲和北美洲的传播时间和地理传播是必要的。寨卡病毒威胁着美国和全球人民的安全。这项研究产生的关键信息将有助于构建准确的预测模型，并确定需要优先考虑公共卫生的高风险领域。 该项目的结果将与寨卡公共卫生紧急情况相关，研究人员已经建立了机制，以尽可能快速和广泛地共享有质量保证的中期和最终数据，包括与公共卫生和研究界。关于寨卡病毒及其与埃及伊蚊相互作用的知识是其外在潜伏期（EIP）和病毒从蚊子传播给其后代的潜力（经卵巢或垂直传播潜力）。 EIP是指从蚊子第一次接触病毒到病毒迁移到唾液腺并且蚊子能够在下次进食时传播病毒的时间长度。 EIP 和寨卡病毒在蚊媒中垂直传播的潜力对于准确模拟病毒在非洲大陆的传播至关重要。研究小组将在不同温度下将蚊子暴露在一系列病毒浓度下，并利用遗传工具来量化病毒并描述蚊子内传播的时间。此外，研究小组还将探讨登革热病毒共同感染如何影响这些变量。