COLLABORATIVE RESEARCH: Dynamics of Circular Macromolecules (DNA): From Single Molecule to Highly Entangled States

合作研究：圆形大分子 (DNA) 动力学：从单分子到高度纠缠态

基本信息

批准号：
1603925
负责人：
Rae Robertson-Anderson
金额：
$ 7.5万
依托单位：
University of San Diego
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2020-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1603925&HistoricalAwards=false
关键词：
COLLABORATIVE RESEARCH Dynamics Circular Macromolecules

项目摘要

PI: McKenna, Gregory B. / Schroeder, Charles / Anderson, RaeProposal Number: 1603943/ 1604038 / 1603925 The goal of this proposal is to explore the behavior of polymer molecules that form large ring, instead of the usual linear polymer molecules. Such polymers, example of which can be the DNA molecule, behave in a different way than linear molecules when processed or when they flow in a solution, because there are not ends in the chains. Results of this work can lead to improved polymer materials, to understanding in detail the behavior of bio-molecules and to new technologies for DNA sequencing. Circular polymers are fascinating materials that have inspired polymer theorists and experimentalists for decades. The dynamics of circular chains differ fundamentally from their linear counterparts due to the absence of chain ends. Despite recent progress, however, the effects of circular topology on polymer dynamics remain a key unresolved problem in the field. In this proposal, the PIs are poised to make major progress in our understanding by preparing circular and linear DNA molecules that are monodisperse and of high topological purity. The assembled team has the expertise to synthesize and characterize circular and linear DNA, and will study the rheological behavior of these materials over a wider range of concentrations and molecular weights than previously achieved. A comprehensive approach is proposed that will include macroscopic and micro-rheology, single molecule polymer dynamics, and DNA synthesis, to provide new information regarding the dynamics of linear and circular DNA. Beyond providing a point of departure for understanding their circular counterparts, the parallel study of linear entangled DNA will provide unprecedented data using perfectly monodisperse DNA samples to directly test predictions from reptation theory, such as the cross-over to reptative behavior at extremely high entanglement densities. In addition to graduate student participation, educational activities are proposed in all three collaborating institutions, ranging from underrepresented minority student involvement at Texas Tech, to high school teacher engagement at Illinois and undergraduate student participation at the U of San Diego, a mainly undergraduate institution.

PI：McKenna，Gregory B. / Schroeder，Charles / Anderson，Raeproposal编号：1603943 /1604038 /1603925该提案的目标是探索形成大环的聚合物分子的行为，而不是通常的线性聚合物分子。这样的聚合物（可以是DNA分子的示例）与加工时线性分子或在溶液中流动时的作用方式不同，因为链中没有末端。这项工作的结果可能会导致改进的聚合物材料，以详细了解生物分子的行为以及用于DNA测序的新技术。圆形聚合物是吸引人的材料，几十年来激发了聚合物理论家和实验者。由于没有链端，圆形链的动力学与线性对应物的根本不同。然而，尽管最近取得了进展，但圆形拓扑对聚合物动力学的影响仍然是该领域的关键尚未解决的问题。在此提案中，PI有望通过制备单分散和高拓扑纯度的圆形和线性DNA分子来取得我们的理解。组装团队具有合成和表征圆形和线性DNA的专业知识，并将研究这些材料在浓度范围更大的浓度和分子量范围内的流变行为。提出了一种全面的方法，该方法将包括宏观和微风格，单分子聚合物动力学和DNA合成，以提供有关线性和圆形DNA动力学的新信息。除了提供出发点以理解其圆形对应物外，线性纠缠DNA的平行研究还将使用完美的单分散DNA样品提供前所未有的数据，以直接测试来自转推理论的预测，例如，在极高的纠缠密度下的交叉至重置行为。除研究生的参与外，在所有三个合作机构中都提出了教育活动，从不足的少数族裔学生参与到德克萨斯理工学院，到伊利诺伊州的高中教师参与以及在圣地亚哥U的本科学生参与，这是一家主要是本科生的学院。

项目成果

期刊论文数量（6）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Topology-dependent anomalous dynamics of ring and linear DNA are sensitive to cytoskeleton crosslinking

DOI：
10.1126/sciadv.aay5912
发表时间：
2019-10
期刊：
Science Advances
影响因子：
13.6
作者：
Devynn M. Wulstein;Kathryn E. Regan;Jonathan Garamella;R. McGorty;R. Robertson-Anderson
通讯作者：
Devynn M. Wulstein;Kathryn E. Regan;Jonathan Garamella;R. McGorty;R. Robertson-Anderson

Viscoelastic properties of ring-linear DNA blends exhibit nonmonotonic dependence on blend composition

环状 DNA 混合物的粘弹性特性表现出对混合物组成的非单调依赖性

DOI：
10.1103/physrevresearch.2.023213
发表时间：
2020
期刊：
Physical Review Research
影响因子：
4.2
作者：
Peddireddy, Karthik R.;Lee, Megan;Schroeder, Charles M.;Robertson-Anderson, Rae M.
通讯作者：
Robertson-Anderson, Rae M.

Effect of molecular architecture on ring polymer dynamics in semidilute linear polymer solutions

DOI：
10.1038/s41467-019-09627-7
发表时间：
2019-04
期刊：
Nature Communications
影响因子：
16.6
作者：
Yuecheng Zhou;K. Hsiao;Kathryn E. Regan;Dejie Kong;G. McKenna;R. Robertson-Anderson;Charles M. Schroeder
通讯作者：
Yuecheng Zhou;K. Hsiao;Kathryn E. Regan;Dejie Kong;G. McKenna;R. Robertson-Anderson;Charles M. Schroeder

Unexpected entanglement dynamics in semidilute blends of supercoiled and ring DNA