DISSERTATION RESEARCH: Mechanisms of host preference in the ectomycorrhizal symbiosis

论文研究：外生菌根共生中宿主偏好的机制

基本信息

批准号：
1600724
负责人：
Kabir Peay
金额：
$ 2.05万
依托单位：
Stanford University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2016
资助国家：
美国
起止时间：
2016-06-01 至 2019-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1600724&HistoricalAwards=false
关键词：
DISSERTATION RESEARCH Mechanisms host preference

项目摘要

Although mutually beneficial interactions are important in many natural systems, it remains unclear why some involve highly specialized partners, while others do not. This project investigates how different degrees of specificity have evolved and function in a particular type of symbiosis - a mutualistic relationship between a fungus and many temperate forest plants. This research will combine a fungus with multiple, distantly related host plants to investigate how the fungus alters its symbiotic behavior when interacting with different species of plants. This experiment will generate insight into symbiotic plant-microbe communication in this important mutualism, and will allow for comparison to similar systems in pathogens. Outreach to science enrichment programs for urban youth in San Francisco will also occur.This study will use RNA sequencing to determine the genetic basis of generalist host preference in the ectomycorrhizal fungus Thelephora terrestris. Six plant species from four families will be established in microcosms and inoculated with spores from Thelephora terrestris. At symbiotic maturity, microcosms will be labeled with carbon-13 and nitrogen-15 and plant, fungal, and symbiotic tissues will be harvested for isotope analysis and RNA sequencing. Transcriptional data will be combined with stable isotope enrichment measurements of plant and fungal resource allocation and compared across different plant-fungus combinations. Measuring gene expression in symbiotic tissues while simultaneously tracking resource allocation will reveal which genes are associated with improved symbiotic performance, providing a powerful link between gene transcription, physiological function, and the stability of mutualistic interactions.

尽管互惠互动在许多自然系统中都很重要，但尚不清楚为什么有些涉及高度专业的伙伴，而另一些伙伴则却没有。该项目研究了不同程度的特异性程度如何发展并在特定类型的共生类型中起作用 - 真菌与许多温带森林植物之间的相互关系。这项研究将将真菌与多种相关的宿主植物结合在一起，以研究该真菌与不同种类的植物相互作用时如何改变其共生行为。该实验将在这种重要的互助中洞悉共生植物 - 微生物通信，并可以与病原体中的类似系统进行比较。旧金山城市青年的科学丰富计划的推广也将发生。这项研究将使用RNA测序来确定通才宿主偏爱的遗传基础。来自四个家族的六种植物物种将在缩影中建立，并接种了Thelephora terrestris的孢子。在共生成熟度时，将对碳13和氮-15和植物，真菌和共生组织标记，以进行同位素分析和RNA测序。转录数据将与植物和真菌资源分配的稳定的同位素富集测量结合在一起，并在不同的植物 - 膜组合中进行比较。在共生组织中测量基因表达，同时跟踪资源分配将揭示哪些基因与改善的共生性能有关，从而在基因转录，生理功能和相互作用的稳定性之间提供强大的联系。