ECA-PGR: DISSECTING THE TRANSCRIPTIONAL NETWORKS UNDERLYING PLANT WOUND SUBERIN BIOSYNTHESIS

ECA-PGR：剖析植物伤口木栓质生物合成背后的转录网络

• 批准号: 1547713
• 负责人: Dylan Kosma
• 金额: $ 137.12万
• 依托单位: Board of Regents, NSHE, obo University of Nevada, Reno
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-15 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1547713&HistoricalAwards=false
• 关键词: ECA; PGR; DISSECTING; TRANSCRIPTIONAL; NETWORKS

Part 1: Non-technical abstract: Potato is one of the top four staple crops in the world and the basis of a more than 4-billion-dollar industry in the U.S. Up to 33% of U.S. potato yield is lost due to post-harvest issues each year. The proper ?healing? or ?curing? of potatoes to fortify their protective skin with the natural polymer suberin is critical for the storage of potatoes. Potato tubers with non-cured bruise or wound areas are much more susceptible to disease development and water loss. The long-term storage of potatoes allows consumers to be supplied with fresh potatoes and potato products (chips, French fries, etc.) year round. By analyzing the regulation of the synthesis and deposition of suberin, this project will provide insight on the fundamental processes underlying the potato skin healing or curing process. This will enable the reduction of post-harvest yield losses and the development of tools and germplasm for breeding efforts aimed at improving tuber storability. Even a marginal reduction in post-harvest storage losses would significantly improve economic return for potato producers and distributors. The project includes as outreach the development of public resources for the scientific and academic (Grades 9-12) communities, and the training of students from underrepresented groups.Part 2: Technical abstract: Suberin is produced In higher plants during normal development and in response to stresses like wounding. Despite its nearly ubiquitous presence, the regulation of suberin deposition remains enigmatic, yet its better understanding has positive agricultural implications. For example, an improvement in the proper deposition of wound suberin, which is critical for long-term storage of potatoes (Solanum tuberosum), would result in a significant reduction in crop loss during storage. The overall objective of this research is to decipher the transcriptional networks that regulate suberin biosynthesis, to enable the development of potato lines with improved wounding and storability. The transcription factor AtMYB41, identified by PI Kosma, and additional factors acting on suberin biosynthesis will be used as a springboard to define the transcriptional networks that regulate suberin deposition, with an emphasis on wound suberization. In parallel, the molecular basis of potato wound suberin production will be investigated using comparative transcriptomic and metabolomic analyses of potato cultivars that differ in suberization and storability. The end goal is to manipulate loci underlying differential wound suberin production by combining transcriptomics and SNP genotyping for targeted breeding approaches aimed at improving tuber storability. The project includes public resources and outreach activities for students from underserved groups.

第1部分：非技术摘要：马铃薯是世界上排名前四的主要农作物之一，并且是美国超过40亿美元的行业的基础，这是由于每年收获后的问题而丢失了美国马铃薯产量的33％。适当的？还是？固化？用天然聚合物suberin强化保护性皮肤的土豆对于储存土豆至关重要。具有非固定瘀伤或伤口区域的马铃薯块茎更容易受到疾病发育和水分流失的影响。土豆的长期存储使消费者全年都提供新鲜的土豆和土豆产品（薯条，炸薯条等）。通过分析Suberin的合成和沉积的调节，该项目将提供有关马铃薯皮肤愈合或固化过程基础的基本过程的见解。这将使收获后产量的损失以及工具的开发和生殖量的开发，以改善块茎的存储性。即使减少收获后存储损失的边缘减少也可以显着改善马铃薯生产者和分销商的经济回报。该项目包括为科学和学术（9 - 12年级）社区开发公共资源的发展，以及对代表性不足的群体的培训。第2部分：技术摘要：Suberin在正常发展期间在较高的植物中生产，并应对诸如伤害等压力。尽管存在几乎普遍存在的存在，但链林沉积的调节仍然是神秘的，但是它更好的理解具有积极的农业意义。例如，改善伤口链林的适当沉积，这对于土豆长期储存至关重要（卵巢结核）至关重要，这将导致储存过程中农作物损失显着减少。这项研究的总体目的是破译调节suberin生物合成的转录网络，以使马铃薯线的开发具有改善的伤害和存储性。由Pi Kosma鉴定的转录因子ATMYB41以及作用于Suberin生物合成的其他因素将用作定义调节Suberin沉积的转录网络的弹簧板，重点是伤口suberization。同时，将使用比较转录组和代谢组对马铃薯品种的比较转录组和代谢组分析来研究马铃薯伤口suberin的分子基础。最终目标是通过结合转录组学和SNP基因分型来操纵基因座的基因座，用于旨在提高块茎栖息性的靶向繁殖方法。该项目包括公共资源和宣传活动，适用于服务不足的团体的学生。