EFRI 2-DARE: Two-dimensional nanopores with electro-optical control for next generation biotechnological applications

EFRI 2-DARE：用于下一代生物技术应用的光电控制二维纳米孔

• 批准号: 1542707
• 负责人: Marija Drndic
• 金额: $ 200万
• 依托单位: University of Pennsylvania
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2021-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1542707&HistoricalAwards=false
• 关键词: EFRI; DARE; Two; dimensional; nanopores

Two-dimensional nanopores can be used to sequence DNA molecules by recording the variations in ionic current and photon emission as the molecules thread through the nanopore. The electronic and optical properties of nanopores in sub-nanometer thick films will be investigated with the goals to understand the nanopore interaction with nanometer-sized biological materials, such as DNA, proteins, and viruses, as well as non-biological materials, such as nanoparticles and nanorod-shaped metals. Using a combination of techniques, the nanopore size and composition will be specifically tuned to optimize sensitivity to a particular analyte. Ultrathin pores in freestanding two-dimensional atomically thin membranes are expected to yield optimal optical and electrical signals that will fingerprint specific nanoparticles as they move through the nanopore. The proposed research will advance knowledge and understanding across different fields and contribute to a number of National Academy of Engineering Grand Challenges, including Engineering the Tools of Scientific Discovery, and Engineering Better Medicines and Advance Health Informatics. Societal impacts include improvement of underrepresented student retention in engineering and science, exposure of K-12 students to state-of-the-art techniques in engineering and science, promoting the participation of women in physics, and training students to make lifelong contributions to technology challenges at the interface of biology, materials science and physics. Two-dimensional materials beyond graphene, such as transition metal dichalcogenides, possess exciting optical, catalytic, electronic and chemical properties. When these layers contain extended vacancies and form nanoscale nanopores, new and unprecedented physicochemical phenomena are expected. Compared to other solid state nanopores, the benefits of these novel two-dimensional materials in nanoparticle detection, filtration (separation) and analysis, include the potential for improved signal-to-noise ratio, operation at high bandwidths (i.e., sub-microsecond temporal resolution), and the prospect that nanopore perimeters can be bestowed with specific, and potentially reversible, edge/surface functionalities. For example, the optical activation and specific chemical functionalization of nanopores opens the possibility of enhanced control of the particle?s translocation rates. The project will target i) the controlled synthesis and characterization of nanopores in homo/heterostructures of two-dimensional materials, ii) the trapping, fragmentation and translocation of DNA, proteins, and bacteria at specific optical wavelengths and optical powers, iii) laser-induced nanopore activation to reversibly control translocation dynamics as well as the native nanopore fluorescence signal to monitor translocation events, iv) the fabrication of prototype translocation platforms for DNA, bioparticulate analysis, and nanoparticle quantification, and v) the elucidation of the trapping/translocation mechanisms for nanopores with designed edge functionalities.

二维纳米孔可用于通过记录离子电流和光子发射的变化来序列DNA分子，因为分子穿过纳米孔。将研究纳米孔厚膜中纳米孔的电子和光学性能，以了解与纳米孔相互作用的目标，以了解与纳米尺寸的生物材料的相互作用，例如DNA，蛋白质和病毒，以及非生物学材料，例如纳米颗粒和纳米棒形金属。使用技术组合，将专门调整纳米孔的大小和成分，以优化对特定分析物的敏感性。独立二维原子薄膜中的超薄孔有望产生最佳的光学信号和电信号，这些信号将在纳米孔中移动时指纹特异性纳米颗粒。拟议的研究将促进各个领域的知识和理解，并为许多国家工程学院的挑战做出贡献，包括工程学的科学发现工具以及工程更好的药物和提高健康信息学。 社会的影响包括改善代表性不足的工程和科学的学生保留率，将K-12学生暴露于工程和科学领域的最先进技术，促进妇女参与物理学，并培训学生在生物学，材料科学和物理学界面上对技术挑战的终身贡献。石墨烯以外的二维材料（例如过渡金属二核苷）具有令人兴奋的光学，催化，电子和化学特性。当这些层包含扩展的空缺并形成纳米级纳米孔时，预计新的和前所未有的物理化学现象。与其他固态纳米孔相比，这些新型二维材料在纳米颗粒检测，过滤（分离）和分析中的好处包括提高信噪比的潜力，在高带宽处的运行（即，亚微共体的时间分辨率），以及具有纳米级的范围/潜在效果，并具有特定于范围的范围。例如，纳米孔的光学激活和特异性化学功能化为增强对粒子易位速率的控制的可能性。 The project will target i) the controlled synthesis and characterization of nanopores in homo/heterostructures of two-dimensional materials, ii) the trapping, fragmentation and translocation of DNA, proteins, and bacteria at specific optical wavelengths and optical powers, iii) laser-induced nanopore activation to reversibly control translocation dynamics as well as the native nanopore fluorescence signal to monitor translocation events, iv）制造用于DNA，生物定位分析和纳米颗粒定量的原型易位平台以及V）具有设计边缘功能的纳米孔的捕获/易位机制的阐明。

项目成果

Engineering adjustable two-pore devices for parallel ion transport and DNA translocations

工程可调双孔装置用于平行离子传输和 DNA 易位

• DOI: 10.1063/5.0044227
• 发表时间: 2021
• 期刊: The Journal of Chemical Physics
• 作者: Chou, Yung-Chien;Chen, Joshua;Lin, Chih-Yuan;Drndić, Marija
• 通讯作者: Drndić, Marija

Single-Stranded DNA Translocation Recordings through Solid-State Nanopores on Glass Chips at 10 MHz Measurement Bandwidth

• DOI: 10.1021/acsnano.9b04626
• 发表时间: 2019-09-01
• 期刊: ACS NANO
• 影响因子: 17.1
• 作者: Chien, Chen-Chi;Shekar, Siddharth;Drndic, Marija
• 通讯作者: Drndic, Marija

Spatial defects nanoengineering for bipolar conductivity in MoS2

• DOI: 10.1038/s41467-020-17241-1
• 发表时间: 2020-07
• 期刊: Nature Communications
• 影响因子: 16.6
• 作者: Xiaorui Zheng;A. Calò;Tengfei Cao;Xiangyu Liu;Zhujun Huang;P. M. Das;M. Drndić;E. Albisetti

Controlled doping of graphene by impurity charge compensation via a polarized ferroelectric polymer

• DOI: 10.1063/5.0003099
• 发表时间: 2020-03
• 期刊: Journal of Applied Physics
• 影响因子: 3.2
• 作者: Kelotchi S. Figueroa;N. Pinto;Srinivas V. Mandyam;Meng-qiang Zhao;C. Wen;Paul Masih Das;Zhaoli Gao;M. Drndić;A. T. Charlie Johnson

Irradiation of Transition Metal Dichalcogenides Using a Focused Ion Beam: Controlled Single‐Atom Defect Creation

• DOI: 10.1002/adfm.201904668
• 发表时间: 2019-08
• 期刊: Advanced Functional Materials
• 影响因子: 19
• 作者: Jothi Priyanka Thiruraman;Paul Masih Das;M. Drndić