MRI: Acquisition of a Fluidigm BioMark HD system for research and training at Humboldt State University

MRI：洪堡州立大学购买 Fluidigm BioMark HD 系统用于研究和培训

• 批准号: 1531910
• 负责人: Andrew Kinziger
• 金额: $ 45.71万
• 依托单位: Humboldt State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531910&HistoricalAwards=false
• 关键词: MRI; Acquisition; Fluidigm; BioMark; HD

An award is made to Humboldt State University (HSU) to acquire a Fluidigm Juno/Biomark system. The Juno/Biomark consists of a real-time Polymerase Chain Reaction (PCR) instrument and a controller designed to load a variety of microfluidic arrays. PCR is a fast method of copying small segments of DNA critical to molecular and genetic analysis. The instrument supports a diverse set of applications, including gene expression, SNP genotyping, mutant detection, and absolute quantification of nucleic-acid sequences utilizing digital PCR. The key feature of the instrumentation are microfluidic arrays that allow thousands of nanoliter reactions to be run in parallel. Several different microfluidic arrays are available, thus the instrument can support users with low as well as high-throughput needs. The instrument will be housed in the College of Natural Resources and Sciences Core Facility, a shared-use laboratory for teaching and research. The Juno/Biomark will serve as a centerpiece for training undergraduate and graduate students in the sciences, including those in the Stem Cell Biology training program and laboratory sections of Behavioral Neuroscience, Cellular Neuroscience, Conservation Genetics, Genetics, and Animal Physiology. Since HSU is a Hispanic Serving Institution, and has a high concentration of Native American students, this project will enable education and training of underrepresented groups with advanced genetic techniques. Collaboration with local teachers and their students at Hoopa High School (in the Hoopa Tribal Nation), McKinleyville High School, and the Redwood Science Institute, an institute which provides training for high school teachers, will provide opportunities for high school students to use this cutting-edge instrument.The Juno/Biomark system will be used by a broad range of researchers across multiple departments and scientific disciplines (Biology, Fisheries, Wildlife, and Psychology) at HSU. The hallmark of the instrumentation is its capacity to support a diversity of applications and its versatility to accommodate different throughput needs, including high-throughput options. Researchers Andrew Kinziger, Sean Craig, Ehtan Gahtan, Micaela Szykman Gunther, Brian Tissot, Darren Ward, Margaret Wilzbach, Jianmin Zhong and colleagues will use the instrumentation to conduct investigations in the areas of conservation and management of fishes, ecology and evolution of invasive species, behavioral neuroscience, behavior and social interactions of mammals, conservation ecology of marine fishes and invertebrates, and detection and control of tick-borne diseases. The new instrumentation will enhance existing research programs and allow investigations that are essentially impossible with existing equipment. The Juno/Biomark system will substantially improve research infrastructure at HSU and the region by providing access to high throughput genetic capabilities to a rural area of northern California far from other universities with comparable instrumentation.

向洪堡州立大学（HSU）颁发了奖励，以获取流畅的Juno/Biomark系统。 JUNO/BIOMARK由实时聚合酶链反应（PCR）仪器和旨在加载各种微流体阵列的控制器组成。 PCR是将关键的DNA小段复制到分子和遗传分析的快速方法。该仪器支持一套多种应用，包括基因表达，SNP基因分型，突变体检测以及利用数字PCR的核酸序列的绝对定量。仪器的关键特征是微流体阵列，该阵列允许并行运行数千个纳米素反应。 提供了几个不同的微流体阵列，因此该仪器可以为具有低通量和高通量需求的用户提供支持。 该工具将安置在自然资源与科学学院核心设施，这是一个共享的教学和研究实验室。 JUNO/BIOMARK将作为培训科学培训本科生和研究生的核心，包括干细胞生物学培训计划和行为神经科学，细胞神经科学，保护遗传学，遗传学和动物生理学的实验室阶层。由于HSU是一家西班牙裔服务机构，并且拥有高度集中的美国原住民学生，因此该项目将对具有先进遗传技术的代表性不足的群体进行教育和培训。 与当地的老师及其学生在Hoopa高中（位于Hoopa Tribal Nation），McKinleyville高中的合作以及为高中教师提供培训的Redwood Science Institute，将为高中生提供这种尖锐的学生提供机会。 该仪器的标志是其能力支持多样化的应用程序及其多功能性，以适应不同的吞吐量需求，包括高通量选项。 研究人员Andrew Kinziger，Sean Craig，Ehtan Gahtan，Micaela Szykman Gunther，Brian Tassot，Brian Tassot，Darren Ward，Darren Ward，Margaret Wilzbach，Jianmin Zhong和同事将利用该工具来在保护和互动的行为，行为，行为，行为，行为，行为，行为和进化的领域进行调查海洋鱼类和无脊椎动物，以及对壁虱传播疾病的检测和控制。 新仪器将增强现有研究计划，并允许现有设备基本上不可能进行调查。 JUNO/BIOMARK系统将通过为远离其他具有可比仪器的其他大学的北加州农村地区提供高通量遗传能力的机会，从而实质上改善了HSU和该地区的研究基础设施。