MRI: Acquisition of a Fluidigm BioMark HD system for research and training at Humboldt State University

MRI：洪堡州立大学购买 Fluidigm BioMark HD 系统用于研究和培训

• 批准号: 1531910
• 负责人: Andrew Kinziger
• 金额: $ 45.71万
• 依托单位: Humboldt State University Foundation
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1531910&HistoricalAwards=false
• 关键词: MRI; Acquisition; Fluidigm; BioMark; HD

An award is made to Humboldt State University (HSU) to acquire a Fluidigm Juno/Biomark system. The Juno/Biomark consists of a real-time Polymerase Chain Reaction (PCR) instrument and a controller designed to load a variety of microfluidic arrays. PCR is a fast method of copying small segments of DNA critical to molecular and genetic analysis. The instrument supports a diverse set of applications, including gene expression, SNP genotyping, mutant detection, and absolute quantification of nucleic-acid sequences utilizing digital PCR. The key feature of the instrumentation are microfluidic arrays that allow thousands of nanoliter reactions to be run in parallel. Several different microfluidic arrays are available, thus the instrument can support users with low as well as high-throughput needs. The instrument will be housed in the College of Natural Resources and Sciences Core Facility, a shared-use laboratory for teaching and research. The Juno/Biomark will serve as a centerpiece for training undergraduate and graduate students in the sciences, including those in the Stem Cell Biology training program and laboratory sections of Behavioral Neuroscience, Cellular Neuroscience, Conservation Genetics, Genetics, and Animal Physiology. Since HSU is a Hispanic Serving Institution, and has a high concentration of Native American students, this project will enable education and training of underrepresented groups with advanced genetic techniques. Collaboration with local teachers and their students at Hoopa High School (in the Hoopa Tribal Nation), McKinleyville High School, and the Redwood Science Institute, an institute which provides training for high school teachers, will provide opportunities for high school students to use this cutting-edge instrument.The Juno/Biomark system will be used by a broad range of researchers across multiple departments and scientific disciplines (Biology, Fisheries, Wildlife, and Psychology) at HSU. The hallmark of the instrumentation is its capacity to support a diversity of applications and its versatility to accommodate different throughput needs, including high-throughput options. Researchers Andrew Kinziger, Sean Craig, Ehtan Gahtan, Micaela Szykman Gunther, Brian Tissot, Darren Ward, Margaret Wilzbach, Jianmin Zhong and colleagues will use the instrumentation to conduct investigations in the areas of conservation and management of fishes, ecology and evolution of invasive species, behavioral neuroscience, behavior and social interactions of mammals, conservation ecology of marine fishes and invertebrates, and detection and control of tick-borne diseases. The new instrumentation will enhance existing research programs and allow investigations that are essentially impossible with existing equipment. The Juno/Biomark system will substantially improve research infrastructure at HSU and the region by providing access to high throughput genetic capabilities to a rural area of northern California far from other universities with comparable instrumentation.

洪堡州立大学 (HSU) 获得了获得 Fluidigm Juno/Biomark 系统的奖励。 Juno/Biomark 由实时聚合酶链反应 (PCR) 仪器和设计用于加载各种微流体阵列的控制器组成。 PCR 是一种快速复制 DNA 小片段的方法，这对分子和遗传分析至关重要。该仪器支持多种应用，包括基因表达、SNP 基因分型、突变体检测以及利用数字 PCR 对核酸序列进行绝对定量。该仪器的主要特点是微流体阵列，可以并行运行数千个纳升反应。 有多种不同的微流体阵列可供选择，因此该仪器可以支持具有低通量和高通量需求的用户。 该仪器将安置在自然资源与科学学院核心设施内，这是一个教学和研究共用实验室。 Juno/Biomark 将作为科学领域本科生和研究生培训的核心，包括干细胞生物学培训项目以及行为神经科学、细胞神经科学、保护遗传学、遗传学和动物生理学实验室部分的学生。由于 HSU 是一所西班牙裔服务机构，并且拥有高度集中的美国原住民学生，因此该项目将为代表性不足的群体提供先进的遗传技术的教育和培训。 与胡帕高中（胡帕部落国家）、麦金利维尔高中和红木科学研究所（一家为高中教师提供培训的机构）的当地教师和学生合作，将为高中生提供使用这种切割的机会-edge 仪器。Juno/Biomark 系统将被 HSU 多个部门和科学学科（生物学、渔业、野生动物和心理学）的广泛研究人员使用。 该仪器的特点是能够支持多种应用，并且具有适应不同通量需求（包括高通量选项）的多功能性。 研究人员 Andrew Kinziger、Sean Craig、Ehtan Gahtan、Micaela Szykman Gunther、Brian Tissot、Darren Ward、Margaret Wilzbach、Jianmin Zhu 及其同事将使用这些仪器在鱼类保护和管理、生态和入侵物种进化等领域进行调查、行为神经科学、哺乳动物的行为和社会互动、海洋鱼类和无脊椎动物的保护生态学以及蜱传疾病的检测和控制。 新仪器将增强现有的研究计划，并允许进行现有设备基本上不可能完成的研究。 Juno/Biomark 系统将为北加州农村地区提供高通量遗传能力，远离其他拥有类似仪器的大学，从而大大改善 HSU 和该地区的研究基础设施。