Acquisition of the Fluidigm system to accelerate functional genomics research

收购 Fluidigm 系统加速功能基因组学研究

• 批准号: 8639787
• 负责人: RUI CHEN
• 金额: $ 39.59万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2015-07-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8639787
• 关键词: Basic Science; Biology; Biomedical Research; Cell Separation; Cells; Clinical Research; Cloning; Core Facility; Coupled; Cytolysis; DNA; Development; Devices; Discipline; Disease; Follow-Up Studies; Gene Expression; Genes; Genetic; Genome; Genomics; Housing; Libraries; Measurement; Measures; Medical; Medicine; Molecular Biology; Patients; Preparation; RNA; Research; Research Personnel; Resolution; Reverse Transcriptase Polymerase Chain Reaction; SNP genotyping; Sampling; San Francisco; Solutions; System; Technology; Time; base; cohort; college; density; functional genomics; genetic variant; genome wide association study; human disease; next generation; next generation sequencing; transcriptome sequencing

DESCRIPTION (provided by applicant): One of the most exciting advances in biomedical research is the introduction of genome scale biology, which enables researchers to gain a global view of their target's genome at high resolution. Rapid development in technologies, such as high density microarrays that enable SNP genotyping and gene expression measurement, the invention of next generation (NextGen) sequencers, and most recently single cell based technology, has greatly accelerated the integration of genome technology into other research fields and has revolutionized the world of molecular biology, genetics, and genomics. One of the biggest challenges is the increasing need for customized, high throughput follow up studies. The requested Fluidigm system (Fluidigm Corporation, San Francisco, CA) is a micro-fluidic device that offers a solution to this critical issue and will greatly accelerate research in a broad rangeof disciplines. Housed in and operated by the established Functional Genomic Core facility at Baylor College of Medicine (BCM), this system will be easily accessed by a large group of researchers and utilized in many different applications, including: 1) GWAS follow up studies to identify disease causing variants and genes from large patient cohorts; 2) high throughput sample SNP genotyping; 3) identifying and cloning human disease genes by regional target capture coupled with the next generation sequencing; 4) high throughput quantitative real time RT-PCR for measuring target gene expression identified from either microarray or RNA-Seq studies; 5) single cell isolation, lysis, DNA/RNA extraction, and library preparation. As the participating PIs engage in both basic and clinical research, including three medical doctors, we believe the introduction of the Fluidigm system will have direct, strong, and immediate impact on many medically relevant fields.

描述（由申请人提供）：生物医学研究中最令人兴奋的进步之一是引入基因组量表生物学，这使研究人员能够以高分辨率对目标的基因组进行全球视野。技术的快速发展，例如高密度微阵列，可以实现SNP基因分型和基因表达测量，下一代（NextGen）序列的发明以及最近基于单细胞的技术，已经大大加速了基因组技术的整合到其他研究领域，并彻底改变了分子生物学，遗传学，遗传学和基因组和基因组。最大的挑战之一是对定制的高通量后续研究的需求越来越多。请求的流体化系统（Fluidigm Corporation，CA旧金山，加利福尼亚州）是一种微荧光设备，为这一关键问题提供了解决方案，并将在广泛的纪律中大大加速研究。由贝勒医学院（BCM）的既定功能基因组核心设施内置并运营，该系统将很容易被大量的研究人员访问，并在许多不同的应用中使用，包括：1）GWAS随访研究，以识别导致大型患者群体的变异和基因的疾病； 2）高吞吐量样品SNP基因分型； 3）通过区域靶标捕获以及下一代测序来识别和克隆人类疾病基因； 4）高吞吐量定量实时RT-PCR，用于测量从微阵列或RNA-Seq研究鉴定的靶基因表达； 5）单细胞分离，裂解，DNA/RNA提取和文库制备。当参与的PI从事基础研究和临床研究（包括三位医生）时，我们认为流体化系统的引入将对许多医学相关领域产生直接，强大和直接的影响。