SusChem: Collaborative Research: Role of Biofilms in Engineered Infiltration Systems in the Removal of Bacteria in Urban Stormwater

SusChem：合作研究：生物膜在工程渗透系统中去除城市雨水细菌中的作用

• 批准号: 1511941
• 负责人: Yusong Li
• 金额: $ 15万
• 依托单位: University of Nebraska-Lincoln
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-15 至 2020-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1511941&HistoricalAwards=false
• 关键词: SusChem; Collaborative; Research; Role; Biofilms

1511915(Chen) & 1511941(Li)Urbanization has resulted in a significant increase in surfaces which prevent stormwater from infiltrating the subsurface and recharging the groundwater. Instead, stormwater accumulates as surface runoff that can result in streambed erosion and flooding events. The runoff can also pick up contaminants (e.g., pathogens) from the surfaces, which eventually enter and contaminate natural aquatic systems. An important component of this research is to investigate the influence of variation in stormwater chemistry and length of drying periods on the chemical properties and microbial diversity of the biofilms and the efficiency of biofilm-modified engineered infiltration systems to remove bacteria during a storm event.This research is the first to systematically study the effects of biofilms grown under environmentally relevant conditions in engineered infiltration systems on the removal of bacteria from stormwater. The changes in the structure, distribution, and microbial diversity of biofilms in porous media grown under a variety of environmental conditions will be revealed for the first time through confocal laser scanning microscopy and high throughput sequencing. The use of atomic force microscopy for the measurements of interfacial forces between a bacterial colloid probe and biofilms will shed light on how a variety of biofilm structures will influence the interfacial interactions between bacteria and biofilm-modified surfaces and the propensity for bacteria to adhere to biofilms. By coupling pore scale and continuous scale modeling, this work will quantitatively link the influence of biofilms on pore scale hydrodynamics with the rate of bacteria transport and attachment at continuum scale. This research will provide new insights on the mechanisms for bacterial retention during filtration, as well as the remobilization of bacteria during draining in biofilm-modified engineered infiltration systems. This research is transformative because it reveals the intricate relationship between environmental conditions, physicochemical properties and microbial diversity of biofilms, and bacteria-biofilm interactions, which will be relevant to the fields of environmental, chemical, and biomedical engineering. The specific tasks include: 1) characterization of biofilms grown under environmentally relevant conditions in microfluidic cells; 2) probing bacterium-biofilm interactions using atomic force microscopy; 3) column filtration experiments including biofilms grown under environmentally relevant conditions; and 4) pore- and continuum-scale modeling of hydrodynamics and bacterial removal in engineered infiltration systems. Research findings will also be incorporated into undergraduate and graduate course materials and further augmented by the involvement of the PIs and their graduate students in organizing scientific activities for third through fifth-grade students in predominantly African American elementary/middle schools in inner-city Baltimore. Short courses on stormwater reuse-related topics will also be developed for the Nebraska EPSCoR's Young Nebraska Scientists summer camps which are attended by K-12 students, a large fraction of them to be underrepresented minorities.

1511915（Chen）和1511941（LI）城市化导致表面显着增加，从而防止雨水渗透地下并为地下水充电。取而代之的是，雨水积聚为表面径流，可能导致河床侵蚀和洪水事件。径流还可以从表面拾起污染物（例如病原体），这些表面最终进入并污染了天然水生系统。这项研究的一个重要组成部分是研究雨水化学变化以及干燥期的变化对生物膜的化学性质和微生物多样性的影响以及生物膜修饰的浸润系统的效率，以在暴风雨事件中去除细菌，这是该研究的第一个系统，该研究是系统地研究生物膜的效应。雨水。在各种环境条件下生长的多孔介质中生物膜的结构，分布和微生物多样性的变化将首次通过共聚焦激光扫描显微镜和高吞吐量测序。使用原子力显微镜在细菌胶体探针和生物膜之间测量界面力的测量将阐明各种生物膜结构将如何影响细菌与生物膜改性表面之间的界面相互作用以及细菌粘附到生物层的倾向。通过将孔尺度和连续尺度建模耦合，这项工作将定量将生物膜对孔隙尺度水动力学的影响与细菌运输和附着的速率以连续尺度的影响联系起来。这项研究将提供有关过滤过程中细菌保留机制的新见解，以及在生物膜修饰工程浸润系统中排出过程中细菌的重新阐明。这项研究具有变革性，因为它揭示了生物膜的环境条件，物理化学特性和微生物多样性与细菌 - 双膜相互作用之间的复杂关系，这将与环境，化学和生物医学工程领域有关。特定任务包括：1）在微流体细胞中与环境相关条件下生长的生物膜的表征； 2）使用原子力显微镜探测细菌 - 双膜相互作用； 3）柱过滤实验，包括在环境相关条件下生长的生物膜； 4）在工程浸润系统中流体动力学和细菌去除的孔隙和连续尺度建模。研究结果还将纳入本科和研究生课程材料中，并通过PIS及其研究生参与的参与进一步增强，以在巴尔的摩市中心的主要非裔美国中学中为三年级至五年级学生组织科学活动。内布拉斯加州EPSCOR的年轻内布拉斯加州科学家夏令营也将开发与雨水与重复相关的主题的短课程，K-12学生参加了大部分，其中很大一部分是代表性不足的少数群体。