Ion Channel Probes for Scanning Ion Conductance Microscopy

用于扫描离子电导显微镜的离子通道探针

基本信息

批准号：
1507341
负责人：
Lane Baker
金额：
$ 44万
依托单位：
Indiana University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2015
资助国家：
美国
起止时间：
2015-07-15 至 2018-06-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1507341&HistoricalAwards=false
关键词：
Ion Channel Probes Scanning Conductance

项目摘要

With this award, the Chemical Measurement and Imaging program of the Chemistry Division is funding Professor Lane Baker at Indiana University to develop chemical/biochemical sensors which consist of lipid bilayers and ion channels suspended at the tip of a glass capillary, called ion channel probes (or ICPs). The sensor will be combined with scanning ion conductance microscopy (SICM), a probe imaging platform, to enable spatial location of the sensor on the order of 50-100 nm. By incorporating ion channels with selective responses, sensors that respond to unique analytes will be developed. The positionable ICP will be tested on porous membranes and will enable measurement of small molecules and ions at subcellular scales and will enable new studies of cellular communication. As such, it is expected that the tools developed in this work that will have broad application in chemical biology. Students trained in this project will learn state-of-the-art fabrication, measurement and characterization protocols, providing them with high level technical skills for the current workforce upon their graduation. In addition, efforts will be made to improve chemistry faculty engagement in evidence-based teaching methods. The proposed research will provide new routes to chemically selective imaging methods, even at single molecule levels, with scanning ion conductance microscopy. This overcomes a significant drawback of scanning ion conductance microscopy; namely, the lack of chemical information collected. The use of inherently selective and sensitive ion channels will impart chemical sensing abilities to scanning ion conductance microscopy. As described, ICP-SICM promises to increase the reliable operation, signal and time response of sniffer patches, which will, in turn, inform the biochemical process they are commonly used to study.

通过此奖项，化学分部的化学测量和成像计划是为印第安纳大学的莱恩·贝克（Lane Baker）提供资金，以开发由脂质双层的化学/生化传感器组成的化学/生化传感器，这些传感器由脂质双层和离子通道组成，该通道悬挂在玻璃毛细管尖端，称为离子通道探测器（或ICP）。传感器将与探针成像平台扫描离子电导显微镜（SICM）结合使用，以使传感器在50-100 nm的订单上启用传感器的空间位置。通过将离子通道与选择性响应结合在一起，将开发对独特分析物的响应的传感器。可定位的ICP将在多孔膜上进行测试，并将在亚细胞尺度上测量小分子和离子，并能够对细胞通信进行新的研究。因此，预计这项工作中开发的工具将在化学生物学中广泛应用。接受该项目培训的学生将学习最先进的制造，测量和表征协议，从而为他们提供高水平的技术技能，以便在毕业后为当前的劳动力提供。此外，将努力在改善基于证据的教学方法中的化学教师参与度。拟议的研究将通过扫描离子电导显微镜为化学选择性成像方法（即使在单分子水平下）提供新的途径。这克服了扫描离子电导显微镜的显着缺点。也就是说，缺乏收集的化学信息。固有选择性和敏感的离子通道的使用将赋予化学感应能力来扫描离子电导显微镜。如上所述，ICP-SICM有望增加嗅探器斑块的可靠操作，信号和时间响应，这又将为它们通常用于研究的生化过程提供信息。