EAGER-Collaborative: Exploring the feasibility of TIRF-based sensors for oceanographic research applications.

EAGER-Collaborative：探索基于 TIRF 的传感器在海洋学研究应用中的可行性。

• 批准号: 1440198
• 负责人: Laurie Connell
• 金额: $ 12.33万
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1440198&HistoricalAwards=false
• 关键词: EAGER; Collaborative; Exploring; feasibility; TIRF

The PIs propose to address the difficulty of developing a small, low power, highly sensitive molecular biology tool for oceanographic work with the novel implementation of a sensor technology known as Total Internal Reflection Fluorescence (TIRF). The TIRF provides rapid, sensitive, simultaneous, and cost-effective detection of multiple biochemical targets with minimal sample preparation. This EAGER proposal seeks funding to explore the feasibility of modifying an existing technology developed by MBio Diagnostics, Inc., consisting of a cartridge and reader system in order to achieve either a smaller form factor, an increased sample capacity, or both. This modified TIRF technology will be designed for integration with an AUV-based sample acquisition/processing module that will provide for underway generation of biological data in support of oceanographic applications.Broader Impacts:The TIRF-based sensor is one of several emerging technologies that promise to transform ocean science by providing the ability to carry out interactive experiments and test hypotheses remotely at the molecular level. It has the potential for providing real or near-real time data revealing not only species? presence and abundance, but also the metabolic and physiological status of those organisms at the biochemical and molecular levels, can provide new insights into how populations are responding in situ to their environment over time as conditions change. This project will continue to build international collaborations with the UK and Ireland. It will include 4 undergraduate and 3 graduate student participation in both the US and Ireland. A workshop will be organized in Belfast as well as using these development experiences as part of an on-line module for a Masters in Diagnostics (University Arizona).

PI 提议通过一种称为全内反射荧光 (TIRF) 的传感器技术的新颖实施来解决开发用于海洋学工作的小型、低功耗、高灵敏度分子生物学工具的困难。 TIRF 能够以最少的样品制备速度快速、灵敏、同步且经济高效地检测多个生化靶标。 该 EAGER 提案寻求资金来探索修改 MBio Diagnostics, Inc. 开发的现有技术的可行性，该技术由盒式磁带和读取器系统组成，以实现更小的外形尺寸、增加的样本容量或两者兼而有之。这种改进的 TIRF 技术将设计用于与基于 AUV 的样本采集/处理模块集成，该模块将提供正在进行的生物数据生成以支持海洋学应用。 更广泛的影响：基于 TIRF 的传感器是有望实现的几种新兴技术之一通过提供在分子水平上远程进行交互式实验和测试假设的能力来改变海洋科学。它有潜力提供实时或近乎实时的数据，不仅揭示物种？这些生物体的存在和丰度，以及这些生物体在生化和分子水平上的代谢和生理状态，可以提供新的见解，了解随着时间的推移，随着条件的变化，种群如何对其环境做出反应。该项目将继续与英国和爱尔兰建立国际合作。它将包括美国和爱尔兰的 4 名本科生和 3 名研究生参与。将在贝尔法斯特组织一个研讨会，并将这些开发经验作为诊断学硕士（亚利桑那大学）在线模块的一部分。