EAGER-Collaborative: Exploring the feasibility of TIRF-based sensors for oceanographic research applications.

EAGER-Collaborative：探索基于 TIRF 的传感器在海洋学研究应用中的可行性。

• 批准号: 1440198
• 负责人: Laurie Connell
• 金额: $ 12.33万
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-06-01 至 2017-05-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1440198&HistoricalAwards=false
• 关键词: EAGER; Collaborative; Exploring; feasibility; TIRF

The PIs propose to address the difficulty of developing a small, low power, highly sensitive molecular biology tool for oceanographic work with the novel implementation of a sensor technology known as Total Internal Reflection Fluorescence (TIRF). The TIRF provides rapid, sensitive, simultaneous, and cost-effective detection of multiple biochemical targets with minimal sample preparation. This EAGER proposal seeks funding to explore the feasibility of modifying an existing technology developed by MBio Diagnostics, Inc., consisting of a cartridge and reader system in order to achieve either a smaller form factor, an increased sample capacity, or both. This modified TIRF technology will be designed for integration with an AUV-based sample acquisition/processing module that will provide for underway generation of biological data in support of oceanographic applications.Broader Impacts:The TIRF-based sensor is one of several emerging technologies that promise to transform ocean science by providing the ability to carry out interactive experiments and test hypotheses remotely at the molecular level. It has the potential for providing real or near-real time data revealing not only species? presence and abundance, but also the metabolic and physiological status of those organisms at the biochemical and molecular levels, can provide new insights into how populations are responding in situ to their environment over time as conditions change. This project will continue to build international collaborations with the UK and Ireland. It will include 4 undergraduate and 3 graduate student participation in both the US and Ireland. A workshop will be organized in Belfast as well as using these development experiences as part of an on-line module for a Masters in Diagnostics (University Arizona).

PI提议通过使用新型的传感器技术（称为总内反射荧光（TIRF））来解决海洋学工作的小型，低功率，高度敏感的分子生物学工具的困难。 TIRF提供了快速，敏感，同时的，并且具有最小的样品制备的多个生化靶标的检测。 这项渴望的建议寻求资金来探索修改由MBIO Diagnostics，Inc。开发的现有技术的可行性，该技术由墨盒和读取器系统组成，以实现较小的形式，更高的样本容量或同时提高。这项修改后的TIRF技术将设计用于与基于AUV的样本采集/处理模块集成，该模块将提供在支持海洋学应用的生物学数据下生成的生成。Broader的影响：基于TIRF的传感器是几种新兴技术之一，是几种有望通过提供互动实验的能力来进行互动实验和测试假设和测试假设的层次级别的分子，该技术有望改变海洋科学。它有可能提供真实或近乎真实的时间数据不仅揭示物种吗？存在和丰度，以及这些生物体在生化和分子水平上的代谢和生理状态，可以提供新的见解，以了解随着条件的变化，随着时间的流逝，种群如何原位对环境的反应。该项目将继续与英国和爱尔兰建立国际合作。它将包括4名本科生和3名研究生参与美国和爱尔兰。将在贝尔法斯特举办一个研讨会，并将这些开发经验用作诊断大师的在线模块的一部分（亚利桑那大学）。