Collaborative Research: A molecular biological sensor for AUVs

合作研究：AUV分子生物传感器

• 批准号: 0961883
• 负责人: Laurie Connell
• 金额: $ 42.1万
• 依托单位: University of Maine
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-01 至 2015-08-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=0961883&HistoricalAwards=false
• 关键词: Collaborative; Research; molecular; biological; sensor; Collaborative; Research; molecular; biological; sensor

Application of molecular analytical techniques for identifying marine microbes, viruses, specific genes, and gene products is central to oceanographic research, monitoring and resource management practices, but generally such methods require transporting samples to a laboratory for processing. This requirement typically results in delays ranging from many hours to days between collection of material and its analysis. Hence, development of instrumentation that overcomes this impediment is seen as a priority. Previously, the PI's approached this problem through development of the Environmental Sample Processor (ESP). The ESP is a field-deployable system that allows for application of molecular probe methodologies remotely in situ, including DNA and protein probe arrays, as well as real-time PCR. Applications of the ESP are currently restricted to moorings or benthic installations that can be embedded within a larger observatory framework. Since the number of moorings that can be deployed at any given time is limited and because they are fixed geographically, the ability to interrogate the "patchiness" of target communities and to assess their dynamics in response to changing environmental conditions from a remote perspective is limited. The PI's propose to overcome this limitation and compliment existing ocean observing technologies by modifying the ESP to fit on mobile platforms. The primary goal of this project is to fit the instrument on a 12" diameter propeller-driven AUV, though the same device could also be integrated on other platforms as well (e.g., profiling mooring or float, drifter). This can be accomplished by repackaging and eliminating some elements of the current ESP, and by combining that device with a field deployable, multi-channel surface plasmon resonance (SPR) sensor. Like the current ESP, the new instrument will be suitable for use with nucleic acid and protein-based assay chemistries, and support sample archiving so that material can be brought back to a laboratory for later analyses. However, its configuration will be aimed at fewer target species/metabolites for a given deployment, consistent with focused AUV reconnaissance operations. Such real-time detection capability will create unique opportunities for oceanographers and resource managers, particularly when placed within an integrated ocean observatory network. Broader ImpactsThe educational and outreach benefits from numerous undergraduates, graduate students, post docs, technical staff, and senior faculty working with the ESPs through various projects; various summer internship programs provided by WHOI and Biosecurity, Inc. for undergraduates including minority students; Monterey Bay Aquarium has included the ESP in a program that runs twice a day reaching almost 300,000 people a year. Although one reviewer described the broader impacts as somewhat predictable, the PI's have a broad base of resources available to them and their ability to utilize these has been demonstrated in the past.

分子分析技术在识别海洋微生物，病毒，特定基因和基因产物中的应用是海洋研究，监测和资源管理实践的核心，但是通常这种方法需要将样品运输到实验室进行处理。这项要求通常会导致延迟范围从材料收集到分析之间的数小时到几天。因此，克服这种障碍的仪器的发展被视为优先事项。以前，PI通过开发环境样品处理器（ESP）解决了这个问题。 ESP是一个可远程部署的系统，允许在原位远程应用分子探针方法，包括DNA和蛋白质探针阵列以及实时PCR。当前，ESP的应用仅限于可以嵌入较大天文台框架中的系泊或底栖安装。由于可以在任何给定时间部署的系泊设备的数量受到限制，并且由于它们在地理上是固定的，因此从远程角度审问目标群落“斑点”并评估其动态的能力是有限的。 PI建议通过修改ESP适合移动平台来克服这一限制并补充现有的海洋观察技术。该项目的主要目标是将仪器放在12英寸的直径螺旋桨驱动的AUV上，尽管同一设备也可以集成在其他平台上（例如，分析型系泊或漂浮，漂流器）。这可以通过将ESP的某些元素重新包装和消除诸如现场置换式的Surpersement surpersemens consons conson（sprane consore consonsemens consone conson）（可以重新包装和消除）来实现。新仪器将适用于核酸和基于蛋白质的测定化学仪式，并支持样品归档，因此可以将材料带回实验室以进行以后的分析，但是，其构型将针对较少的目标物种/代谢物进行给定的部署，以使其与侦察台保持一致。更广泛地影响了众多本科生，研究生，文档，技术人员以及通过各种项目与ESPS合作的高级教职员工受到教育和外展的影响； Whoi and Biosecurity，Inc。为包括少数族裔学生在内的本科生提供的各种暑期实习计划；蒙特利湾水族馆（Monterey Bay Aquarium）将ESP纳入了一项计划，该计划每天两次运行两次，每年达到30万人。尽管一位审稿人将更广泛的影响描述为可以预见的，但PI具有广泛的资源基础，并且过去已经证明了他们利用这些资源的能力。