MRI: Acquisition of an automatic NMR sample changer

MRI：购买自动 NMR 样品更换器

• 批准号: 1429353
• 负责人: Arezue Boroujerdi
• 金额: $ 5.03万
• 依托单位: Claflin University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2017-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1429353&HistoricalAwards=false
• 关键词: MRI; Acquisition; automatic; NMR; sample

An award is made to Claflin University for the purchase of an automated nuclear magnetic resonance (NMR) sample changer to do NMR-based metabolomics research. The field of metabolomics focuses on changes to the small molecules that make up metabolism upon some stressful event or condition. NMR serves as an excellent detector of these small compounds; and statistical analysis is applied to many NMR sample replicates to increase the robustness of the metabolomics technique. The addition of an automated sample changer to Claflin?s existing 700 MHz NMR not only will make metabolomics research more efficient, but also the NMR will be more user-friendly. Specifically, the SampleCaseTM is mounted to the instrument at user height and eliminates the use of a ladder. Undergraduate students will gain more access to collecting NMR data and be trained in cutting-edge NMR-based metabolomics research. Claflin University has the third highest field NMR laboratory in the state of South Carolina, just behind the Medical University of South Carolina (MUSC) and the Hollings Marine Laboratory (HML). Although a predominately undergraduate university and HBCU (Historically Black Colleges and Universities), Claflin's NMR facility is becoming a state and nation wide shared facility with six collaborative metabolomics projects and plans to secure major users of the instrument from other future collaborative partners. The automated sample changer will enhance the current instrument capabilities, and is in line with the chemistry department's mission to not only become a state level shared NMR facility, but to support and enhance undergraduate research participation. With the addition of an automatic sample changer, Claflin's NMR facility will be more attractive to outside users as well as be able to accommodate multiple undergraduate metabolomics research projects. Housed in the Molecular Science and Research Center (MSRC) on Claflin?s campus, the extraordinary collection of state of the art instrumentation, including the 700 MHz Bruker NMR spectrometer, is unmatched by any other HBCU. PI Boroujerdi's laboratory specializes in NMR-based metabolomics research; however, with such a high field magnet, the capability to collect and analyze large bio-molecular samples via NMR is possible and open to any external users.Claflin undergraduates love NMR-based metabolomics research. PI Boroujerdi is the newest biochemistry faculty member at Claflin, and since the beginning of her tenure three years ago, her metabolomics group has grown exponentially (currently with to 14 undergraduate students, 4 graduate students, and 1 staff). The nature of metabolomics lends itself to collaborations which is ideal for Claflin undergraduates whose research interest are spread far and wide in basic biology research. With 6 collaborators across the nation, and the recent interest in metabolomics research at Claflin, the demand for the 700 MHz NMR has dramatically increased to the point of saturation. With automatic sample collection, the large number of routine one dimensional 1H spectra can be collected more efficiently, and the extremely inefficient personnel time wasted on manual sample insertion and data collection will be refocused on bench work and data analysis, further increasing the quality and quantity of data produced by the metabolomics group. In order to accommodate the increasing number of undergraduates participating in metabolomics research, which includes the requirement for science majors to write and defend a senior thesis prior to graduation, many projects and collaborators have been established, which translates into a large number of NMR samples and a need for more efficient NMR data collection. Sample preparation, interpretation of results, manuscript writing, and presenting results will be increased with the addition of an automated sample changer. Instead of devoting personnel time to manual routine NMR data collection, while data is collected in automation, PI Boroujerdi can devote her time to training undergraduates in metabolite extraction techniques, statistical analysis of NMR data, and combing the literature to understand the biological implications of the metabolomics results.

克拉夫林大学因购买自动核磁共振 (NMR) 进样器以进行基于 NMR 的代谢组学研究而获得奖励。代谢组学领域专注于在某些应激事件或条件下构成新陈代谢的小分子的变化。 NMR 是这些小化合物的优秀检测器；统计分析应用于许多 NMR 样品重复，以提高代谢组学技术的稳健性。在 Claflin 现有的 700 MHz NMR 中添加自动进样器不仅将使代谢组学研究更加高效，而且 NMR 也将更加用户友好。具体来说，SampleCaseTM 安装在仪器的用户高度处，无需使用梯子。本科生将有更多机会收集 NMR 数据，并接受基于 NMR 的尖端代谢组学研究培训。克拉夫林大学拥有南卡罗来纳州第三高的现场核磁共振实验室，仅次于南卡罗来纳医科大学 (MUSC) 和霍林斯海洋实验室 (HML)。虽然克拉夫林的 NMR 设施主要是一所本科大学和 HBCU（历史上的黑人学院和大学），但它正在成为一个州和全国范围内的共享设施，拥有六个协作代谢组学项目，并计划从其他未来的合作伙伴那里确保该仪器的主要用户。自动进样器将增强当前的仪器功能，并且符合化学系的使命，即不仅成为国家级共享核磁共振设施，而且支持和增强本科生的研究参与。通过添加自动进样器，Claflin 的 NMR 设施将对外部用户更具吸引力，并且能够容纳多个本科生代谢组学研究项目。位于克拉夫林校区的分子科学研究中心 (MSRC)，拥有一系列最先进的仪器，包括 700 MHz 布鲁克核磁共振波谱仪，是任何其他 HBCU 无法比拟的。 PI Boroujerdi 的实验室专门从事基于 NMR 的代谢组学研究；然而，有了如此强的磁场，通过 NMR 收集和分析大型生物分子样本的能力是可能的，并且向任何外部用户开放。克拉夫林的本科生喜欢基于 NMR 的代谢组学研究。 PI Boroujerdi 是 Claflin 最新的生物化学教员，自三年前上任以来，她的代谢组学团队呈指数级增长（目前有 14 名本科生、4 名研究生和 1 名工作人员）。代谢组学的性质适合于合作，这对于克拉夫林本科生来说是理想的选择，因为他们的研究兴趣广泛分布在基础生物学研究中。全国有 6 个合作者，加上 Claflin 最近对代谢组学研究的兴趣，对 700 MHz NMR 的需求急剧增加至饱和点。通过自动样品采集，可以更高效地采集大量常规一维1H谱，将浪费在手动样品插入和数据采集上的极其低效的人员时间重新集中在实验室工作和数据分析上，进一步提高质量和数量代谢组学小组产生的数据。为了适应越来越多的本科生参与代谢组学研究，包括理科专业学生在毕业前撰写和答辩高级论文的要求，已经建立了许多项目和合作者，这转化为大量的 NMR 样本和需要更有效的 NMR 数据收集。通过添加自动样品更换器，样品制备、结果解释、手稿撰写和结果呈现将会得到加强。 PI Boroujerdi 无需将人员时间用于手动常规 NMR 数据收集，而是通过自动化收集数据，她可以将时间投入到培训本科生代谢物提取技术、NMR 数据统计分析以及梳理文献以了解代谢物提取技术的生物学含义。代谢组学结果。