Evaluation of the Role of Size Dispersity on Nanoparticle Uptake and Ecotoxicity

尺寸分散性对纳米颗粒吸收和生态毒性作用的评估

• 批准号: 1437307
• 负责人: Mohammed Baalousha
• 金额: $ 29.99万
• 依托单位: University of South Carolina at Columbia
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-08-01 至 2018-07-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1437307&HistoricalAwards=false
• 关键词: Evaluation; Role; Size; Dispersity; Nanoparticle

PI: Baalousha, MohammedProposal Number: 1437307 Institution: University of South Carolina at Columbia Title: Evaluation of the Role of Size Dispersity on Nanoparticle Uptake and EcotoxicityStudies of nanoparticle (NP) toxicity to the environment often come up with contradictory results. For example, nanoparticles (NPs) have demonstrable negative impact on aquatic organisms, but divergent results have been reported on the role of NP size on their toxicity. The PI and Co-PIs hypothesize that such contradictory evidence is most likely due to the heterogeneity and polydispersity of the NPs themselves. They hypothesize that polydispersity can cause incorrect measurement of NP properties and lead to an underestimation of NP dose, particularly if the toxic effects are only due to a sub-fraction of the polydisperse suspensions. The proposal will specifically investigate the effect of NP dispersity on their properties and on their toxicities, investigating such NP properties as size, dispersity, morphology, and facet exposure, and their effect in nanotoxicity. The results of this project will help improve the design of NPs by identifying features that can induce deleterious effects, and also help Understand the fate, behavior, uptake, and toxicity of NPs. Progress in this area will aid a sustainable development of nanotechnology and its benefits. Under this proposal's plan, the PIs will work directly with K-12 institutions and underrepresented minorities (URMs) in South Carolina and produce distance-learning materials for wider outreach. The PIs have a strong track record of ensuring inclusion of under-represented groups in training and outreach activities and will recruit for the PhD candidate with outreach principles in mind. The University of South Carolina has one of the highest numbers of African American students for a Carnegie One research intensive university (13%).To achieve their goals, the PIs must first rigorously characterize the NPs they synthesize and then correlate the various properties of the NPs with their observed toxicities. To that end, the PIs will seek to establish a reproducible protocol for the synthesis of poly- and mono-dispersed NPs and their separation into a set of monodisperse subfractions. They will then characterize the NPs (e.g., size, dispersity, morphology, facets) using a multi-method approach and quantify the uptake and toxicity of polydisperse versus monodisperse subfractions of NPs using the standardized polyhaline estuarine copepod model, Amphiascus tenuiremis.With this approach, the PI and Co-PIs seek to provide a methodology that can be widely used by the scientific community for similar and other studies, as well as in other fields such as drug delivery, where NP size dispersity has been realized as an issue of confounding results in the literature.

PI：Baalousha，Mohammedproposal编号：1437307机构：南卡罗来纳大学哥伦比亚大学的标题：纳米粒子摄取和纳米粒子毒性研究的尺寸分散性对环境的毒性（NP）毒性的评估通常会带来相反的结果。例如，纳米颗粒（NP）对水生生物具有明显的负面影响，但已经报道了NP大小对其毒性的作用的不同结果。 PI和Co-Pis假设这种矛盾的证据很可能是由于NPS本身的异质性和多分散性。他们假设多分散性会导致NP特性的不正确测量并导致NP剂量低估，尤其是在毒性作用仅是由于多分散悬浮液的亚分数所致的情况下。该提案将专门研究NP分散性对其性质及其毒性的影响，研究诸如大小，分散性，形态和面部暴露等NP特性及其对纳米毒性的影响。该项目的结果将通过识别可以引起有害影响的功能，并帮助了解NP的命运，行为，摄取和毒性来帮助改善NP的设计。该领域的进展将有助于纳米技术及其利益的可持续发展。根据该提案的计划，PIS将直接与南卡罗来纳州的K-12机构和代表性不足的少数民族（URMS）合作，并生产远程学习材料以进行更广泛的外展。 PI在确保将代表性不足的群体包括在培训和推广活动中的良好记录中，并将招募有外展原则的博士候选人。南卡罗来纳大学（University of South Carolina）是卡内基一所研究强化大学（13％）的非洲裔美国学生人数最多的。为了实现其目标，PIS必须首先严格地将其合成的NP特征在内，然后将NP的各种特性与观察到的毒性相关联。为此，PI将寻求建立可重现的协议，以合成聚和单分散的NP，并将其分离为一组单分散亚折。然后，他们将使用多方法方法来表征NP（例如尺寸，分散性，形态，形态，方面），并量化多形分散和单分散的毒性和单分散的毒性，并使用标准化的polyhaline Estuarine Estuarine copepod来使用AmphiasCuscus.tenuiremis。用于类似研究和其他研究的科学界以及其他领域（例如药物输送），在这些领域中，NP大小分散性已被视为文献中混淆结果的问题。

项目成果

Particle size determines the accumulation of platinum nanoparticles in the estuarine amphipod, Leptocheirus plumulosus

粒径决定铂纳米颗粒在河口端足类动物 Leptocheirus penulosus 中的积累

• DOI: 10.1039/d1en00713k
• 发表时间: 2022
• 期刊: Environmental Science: Nano
• 作者: Sikder, Mithun;Eudy, Emily;Cai, Bo;Chandler, G. Thomas;Baalousha, Mohammed
• 通讯作者: Baalousha, Mohammed

The concentration-dependent aggregation of Ag NPs induced by cystine

• DOI: 10.1016/j.scitotenv.2016.02.212
• 发表时间: 2016-07-01
• 期刊: SCIENCE OF THE TOTAL ENVIRONMENT
• 影响因子: 9.8
• 作者: Afshinnia, K.;Gibson, I.;Baalousha, M.
• 通讯作者: Baalousha, M.

Potential impact of natural organic ligands on the colloidal stability of silver nanoparticles

• DOI: 10.1016/j.scitotenv.2017.12.299
• 发表时间: 2018-06-01
• 期刊: SCIENCE OF THE TOTAL ENVIRONMENT
• 影响因子: 9.8
• 作者: Afshinnia, Kamelia;Marrone, Brandon;Baalousha, Mohammed
• 通讯作者: Baalousha, Mohammed

Comparative study of dissolved and nanoparticulate Ag effects on the life cycle of an estuarine meiobenthic copepod, Amphiascus tenuiremis

溶解银和纳米颗粒银对河口小型底栖桡足动物 Amphiascus tenuiremis 生命周期影响的比较研究

• DOI: 10.1080/17435390.2018.1451568
• 发表时间: 2018
• 期刊: Nanotoxicology
• 影响因子: 5
• 作者: Sikder, Mithun;Eudy, Emily;Chandler, G. Thomas;Baalousha, Mohammed
• 通讯作者: Baalousha, Mohammed

Effect of phosphate buffer on aggregation kinetics of citrate-coated silver nanoparticles induced by monovalent and divalent electrolytes

• DOI: 10.1016/j.scitotenv.2016.12.117
• 发表时间: 2017-03-01
• 期刊: SCIENCE OF THE TOTAL ENVIRONMENT
• 影响因子: 9.8
• 作者: Afshinnia, K.;Baalousha, M.
• 通讯作者: Baalousha, M.