Cold Spring Harbor Laboratory Course on Yeast Genetics and Genomics

冷泉港酵母遗传学和基因组学实验室课程

• 批准号: 1437145
• 负责人: David Stewart
• 金额: $ 25.5万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-15 至 2017-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1437145&HistoricalAwards=false
• 关键词: Cold; Spring; Harbor; Laboratory; Course

The Yeast Genetics and Genomics Course serves a critical role in the training of investigators new to the use of baker's yeast (Saccharomyces cerevisiae) for biological research. Yeast has long been, and continues to be, an outstanding organism for many different types of studies, ranging from basic research on genetics and cell biology to applied research on biotechnological, pharmaceutical, or industrial uses. The highly intensive three-week course is attractive to researchers, as it offers an opportunity to become proficient in the fundamentals of yeast genetics and state-of-the-art uses of this important organism. The broader impact of the course is the training of researchers in the most up-to-date experimental and computational resources and tools available for biological research in the yeast model. Students in the course are diverse, ranging from graduate students to professors, with backgrounds in genetics, molecular and cellular biology, bioinformatics, engineering, and physics. The diversity of their backgrounds and their exposure to a wide range of experimental approaches during the course increases the chances that they will go on to carry out the interdisciplinary research that is vital for meeting today's global challenges. The course includes laboratory experiments that are broken into twelve multi-part sections that cover a variety of topics including, fundamentals of working with yeast, classical genetics and related topics in cell biology, introduction to yeast molecular and cell biology, genetic and physical interaction analysis, and genomics. The experimental portion of the course is complemented by daily theoretical lectures by instructors chosen for their proven expertise in the system and by a world-class seminar series from renowned investigators that utilize the yeast system. Although the overall organization of the course remains the same from year to year, changes in content are introduced as yeast biology and genomics advances; novel technologies and approaches are incorporated into the curriculum, in part by the recruitment of new instructors. However, because at least two instructors remain from year to year, this evolution does not come at the sacrifice of continuity. The course enrolls 16 students per year, who are chosen from a large pool of applicants using criteria that emphasize need, diversity, and commitment to broadly disseminate information acquired in the course. Many of the students are likely to have educational and supervisory responsibilities in the near future; others are already professors or post-doctoral fellows who will immediately pass on what they have learned in the course. In recent years, there has been a good balance of female and male students, and several under-represented minority students have participated. The course is advertised widely and specific contacts are made to institutional programs that facilitate recruitment and retention of underrepresented groups in the life sciences. Periodically, the experimental protocols and associated materials are published in the form of a laboratory manual, and reach a wider audience through that mechanism.

酵母遗传学和基因组学课程在培训新使用面包酵母（酿酒酵母）进行生物学研究的研究人员方面发挥着关键作用。酵母长期以来一直是并且仍然是许多不同类型研究的杰出生物体，从遗传学和细胞生物学的基础研究到生物技术、制药或工业用途的应用研究。为期三周的高度强化课程对研究人员很有吸引力，因为它提供了精通酵母遗传学基础知识和这种重要生物体的最先进用途的机会。该课程更广泛的影响是对研究人员进行酵母模型生物学研究可用的最新实验和计算资源和工具的培训。该课程的学生多种多样，从研究生到教授，都有遗传学、分子和细胞生物学、生物信息学、工程学和物理学的背景。他们背景的多样性以及在课程期间接触到的各种实验方法增加了他们继续进行跨学科研究的机会，这对于应对当今的全球挑战至关重要。该课程包括实验室实验，分为十二个多部分，涵盖各种主题，包括酵母工作基础知识、经典遗传学和细胞生物学相关主题、酵母分子和细胞生物学简介、遗传和物理相互作用分析和基因组学。课程的实验部分辅以每日理论讲座（由因其在系统方面经过验证的专业知识而挑选的讲师进行）以及由利用酵母系统的著名研究人员举办的世界级研讨会系列进行补充。尽管课程的整体组织每年都保持不变，但随着酵母生物学和基因组学的进步，内容也会发生变化；新技术和方法被纳入课程中，部分是通过招聘新教师来实现的。然而，由于每年至少有两名教练留任，这种演变不会以牺牲连续性为代价。该课程每年招收 16 名学生，这些学生是从大量申请者中挑选出来的，其标准强调需求、多样性以及广泛传播课程中获得的信息的承诺。 许多学生可能在不久的将来承担教育和监督的职责；其他人已经是教授或博士后研究员，他们将立即传授他们在课程中学到的知识。近年来，男女学生比例保持良好，也有一些少数族裔学生参与其中。该课程得到广泛宣传，并与促进生命科学领域代表性不足群体的招募和保留的机构计划建立了具体联系。实验方案和相关材料会定期以实验室手册的形式发布，并通过该机制覆盖更广泛的受众。