CSHL Drosophila Neurobiology: Genes, Circuits & Behavior

CSHL 果蝇神经生物学：基因、回路

• 批准号: 1949855
• 负责人: David Stewart
• 金额: $ 41.27万
• 依托单位: Cold Spring Harbor Laboratory
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2024-10-31
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1949855&HistoricalAwards=false
• 关键词: CSHL; Drosophila; Neurobiology; Genes; Circuits

The "Neurobiology of Drosophila: Genes, Circuits and Behavior" course (2020-2023) is an intensive advanced three week summer course taught by leading neuroscientists with extensive and complementary research experience studying brain function in this important model organism. The course is taught by lead instructors who remain at Cold Spring Harbor Laboratory (CSHL) throughout the duration of the course together with teaching assistants from their laboratories. Twelve students will be selected from a pool of applicants largely on the basis of scientific merit. Course trainees include advanced graduate students (typically 70%), postdoctoral trainees (typically 25%) and junior faculty (5%) who are entering into neurobiological research using the fly model. Women and underrepresented minorities will be actively recruited. The course has a broad impact on the field in that students are chosen based in part on their potential for disseminating the acquired skills and knowledge at their home institutions. The course also provides instructors, assistants and guest lecturers with opportunities to explore new approaches and material for teaching neuroscience with Drosophila at their universities. Protocols developed in the course will reach an audience beyond the classroom through publication of laboratory manuals (Drosophila Neurobiology: A Laboratory Manual) and technique videos that will be made available on CSHL's E-Technical web resource.The course consists of laboratory sessions led by 3 course directors and daily lectures presented by up to 20 leading experts in the field. The hands-on portion of the course is centered on inquiry-based projects, utilizing different morphological and physiological measurements and behavioral paradigms learned in the course. Specific techniques include molecular genetic analyses, quantitative neuroanatomy, computational modeling, recording of neural activity using electrophysiology and genetically encoded calcium indicators, optogenetic and thermogenetic control of neural activity, as well as numerous quantitative behavioral measures. Guest lecturers will divide their presentations into two parts. The first part will address the core neurobiological concepts and techniques they use in their own research, and the second part will focus on how their specific research fits into the field as a whole. Collectively, the course will provide a comprehensive and practical introduction to modern experimental methods for studying the neural basis of behavior in Drosophila. Long-term impact analysis demonstrates that this course enhances research productivity, builds collaborations, and reaches a broader audience through dissemination of protocols and approaches.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

“果蝇神经生物学：基因、回路和行为”课程（2020-2023）是一门为期三周的强化高级夏季课程，由领先的神经科学家讲授，他们在研究这一重要模式生物的大脑功能方面拥有广泛和互补的研究经验。该课程由在整个课程期间留在冷泉港实验室 (CSHL) 的首席讲师及其实验室的助教教授。十二名学生将主要根据科学价值从申请者中选出。课程学员包括高级研究生（通常占 70%）、博士后学员（通常占 25%）和初级教师（5%），他们正在使用果蝇模型进行神经生物学研究。将积极招募妇女和代表性不足的少数族裔。该课程对该领域具有广泛的影响，因为选择学生的部分原因是他们在本国机构传播所获得的技能和知识的潜力。该课程还为讲师、助理和客座讲师提供了探索在大学中用果蝇教授神经科学的新方法和材料的机会。本课程中制定的协议将通过出版实验室手册（果蝇神经生物学：实验室手册）和技术视频（将在 CSHL 的电子技术网络资源上提供）向课堂以外的受众传达。该课程包括由 3 位领导者主持的实验室课程课程主管和每日讲座由多达 20 位该领域的领先专家主讲。课程的实践部分以基于探究的项目为中心，利用课程中学到的不同形态和生理测量以及行为范式。具体技术包括分子遗传学分析、定量神经解剖学、计算建模、使用电生理学和基因编码钙指示剂记录神经活动、神经活动的光遗传学和热遗传学控制以及许多定量行为测量。客座讲师将把他们的演讲分为两个部分。第一部分将讨论他们在自己的研究中使用的核心神经生物学概念和技术，第二部分将重点讨论他们的具体研究如何适应整个领域。总的来说，该课程将全面、实用地介绍研究果蝇行为神经基础的现代实验方法。长期影响分析表明，本课程通过传播协议和方法提高了研究生产力、建立了合作并覆盖了更广泛的受众。该奖项反映了 NSF 的法定使命，并通过使用基金会的智力优势和更广泛的评估进行评估，被认为值得支持。影响审查标准。