Spatially precise optogenetics at depth: cracking neural circuits with structured illumination and ultrafast imaging in the intact brain. DEC 8-10, 2013; Workshop

深度空间精确光遗传学：利用结构化照明和超快成像在完整大脑中破解神经回路。

基本信息

批准号：
1412497
负责人：
Elizabeth Rogan
金额：
$ 1万
依托单位：
Optical Society of America
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2013
资助国家：
美国
起止时间：
2013-12-01 至 2014-11-30
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1412497&HistoricalAwards=false
关键词：
Spatially precise optogenetics depth cracking

项目摘要

Optogenetics is rapidly altering how scientists reveal the basic operation of the brain circuits that are central to sensation, action, and cognition. The vast majority of optogenetic studies to date have primarily capitalized on the genetic side of optogenetics taking advantage of the specificity of gene expression tocontrol and image unique cell types and connections in the brain. However, precise spatialcontrol of the illuminating light affords an additional dimension of specificity that has been underutilized, partly owing to the challenges posed by the scattering properties of brain tissue. Recent and future work in multi-photon imaging and structured illumination, combined with alternative approaches such as micron scale, implantable illumination devices promise to open up whole new avenues of research to understand the elementary basis of neural computation, sensory coding, and neural defects in brain diseases such as autism, schizophrenia, and epilepsy. There will a focus at this meeting:on advances in structured illumination, ultrafast imaging and local implantable illumination devices, to control neural activity at brain depths beyond the limits of multi-photon microscopy. Invitees will present their recent advances, and also discuss the major outstanding challenges in this field and how to surmount thesechallenges to develop the necessary technologies that will unlock fundamental aspects of brain function and disease.

光遗传学正在迅速改变科学家如何揭示脑电路的基本操作，这是感觉，动作和认知的核心。迄今为止，绝大多数的光遗传学研究主要利用了光遗传学的遗传侧，利用了基因表达对控制的特异性和图像在大脑中独特的细胞类型和连接。然而，照明光的精确空间孔子提供了一个额外的特异性维度，部分原因是由于脑组织的散射特性所带来的挑战，部分原因是。多光子成像和结构化照明中的最新和未来工作，结合了Micron量表，可植入的照明设备等替代方法，有望开放全新的研究途径，以了解神经计算，感觉编码，感官编码和神经缺陷的基本基础，例如自闭症，精神分裂症和伯爵夫人。这次会议将重点：关于结构化照明，超快成像和局部植入照明设备的进步，以控制超出多光子显微镜限制的脑深度的神经活动。 Invitees将提出他们的最新进展，并讨论该领域的主要杰出挑战，以及如何克服该领域的Thesechallenges来开发必要的技术，这些技术将释放大脑功能和疾病的基本方面。