Dissertation Research: The role of gene duplication and symbiont association in the evolution of sea anemone venom

论文研究：基因复制和共生体关联在海葵毒液进化中的作用

基本信息

批准号：
1401014
负责人：
Marymegan Daly
金额：
$ 1.97万
依托单位：
Ohio State University
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-01 至 2017-05-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1401014&HistoricalAwards=false
关键词：
Dissertation Research role gene duplication

项目摘要

The diversity of venoms within a group of organisms is largely the product of gene duplication and subsequent DNA sequence diversification. The specifics of this process remain obscure in most organisms. The planned research uses sea anemones (Cnidaria: Anthozoa, Actiniaria) as a model to understand the genome-level processes like gene duplications in shaping venom repertoire. The incidence of gene duplication and the processes by which duplicated genes diversify or become non-functional can be inferred through the analysis of the sequences that flank functional venom genes and the regions of the genome in which venoms are particularly common.Sea anemones are part of the oldest and largest group of extant venomous animals. Their venoms exhibit a variety of functions, including disrupting the control of ion channels, destabilizing cellular membranes, or inhibiting synthesis of lipids. The functional and structural diversity of venoms is underpinned by an even greater degree of diversity at the genetic level. This more detailed account of venom diversity thus has potential for studies of function and structure of venom, and may have applications of interest to pharmacological or medical research. Additional benefits to the broader community include the training of graduate and undergraduate students, outreach to general and technical audiences through presentations, and published scientific studies of gene diversity and evolution.

一组生物体内毒液的多样性很大程度上是基因复制和随后的 DNA 序列多样化的产物。在大多数生物体中，这个过程的细节仍然不清楚。计划中的研究使用海葵（刺胞动物：Anthozoa、Actiniaria）作为模型来了解基因组水平的过程，例如形成毒液库的基因复制。通过分析功能性毒液基因侧翼的序列以及毒液特别常见的基因组区域，可以推断出基因复制的发生率以及复制基因多样化或变得无功能的过程。海葵是现存最古老和最大的有毒动物群。它们的毒液具有多种功能，包括破坏离子通道的控制、破坏细胞膜的稳定或抑制脂质的合成。毒液的功能和结构多样性是由基因水平上更大程度的多样性支撑的。因此，这种对毒液多样性的更详细的描述具有研究毒液功能和结构的潜力，并且可能在药理学或医学研究中具有应用价值。对更广泛社区的其他好处包括培训研究生和本科生、通过演讲向普通和技术受众进行宣传以及发表关于基因多样性和进化的科学研究。