DISSERTATION RESEARCH: Uncovering the geographic pattern of species evolution in Madagascar using the myrrh genus (Commiphora Jacq.) and a target-enriched nuclear genomic approach

论文研究：利用没药属（Commiphora Jacq.）和目标富集核基因组方法揭示马达加斯加物种进化的地理模式

• 批准号: 1403150
• 负责人: Andrea Weeks
• 金额: $ 1.93万
• 依托单位: George Mason University
• 依托单位国家: 美国
• 项目类别: Standard Grant
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-07-01 至 2016-06-30
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=1403150&HistoricalAwards=false
• 关键词: DISSERTATION; RESEARCH; Uncovering; geographic; pattern

In the last decade, scientific exploration of Madagascar's remarkable biodiversity and its origin has shifted to characterizing the drivers of evolution within the island. This project will use two endemic lineages of the myrrh tree genus (50 spp., Commiphora, Burseraceae) as models to test hypotheses regarding the influence of geography on species diversification in the seasonally dry tropical forests of western Madagascar. These hypotheses predict that species have arisen as a consequence of isolation caused by 1) watershed expansion and contraction, 2) river barriers, and 3) historical climate change. A subset of 959 nuclear genes will be sampled from all endemic species using a cost-effective high-throughput method that combines microfluidic amplification and massively-parallel sequencing. The resulting data will be used to reconstruct the evolutionary history of these species and will be combined with specimen-based mapping to test the overarching biogeographical hypotheses. Genomic markers developed as part of this research will also be evaluated for their transferability to other species across the angiosperm tree of life, which may expand the methodological toolkit for all plant systematists. This research will provide mentorship opportunities for two undergraduate students and will support their professional development, including presentation of their research at a national meeting. Additional outreach will be provided to involve K-12 and undergraduate students through ongoing mentorship activities.

在过去的十年中，对马达加斯加的非凡生物多样性及其起源的科学探索已转向岛上进化的驱动力。该项目将使用MyRRH树属（50 spp。，commiphora，burseraceae）的两个地方性谱系作为模型，以测试有关地马达加斯加季节性干燥热带森林中地理对物种多样化的影响的假设。这些假设预测，由于1）分水岭的扩张和收缩，2）河流障碍和3）历史气候变化而预测，由于1）流域扩张和收缩而产生的物种。 将使用具有成本效益的高通量方法从所有地方性物种中取样959个核基因的子集，该方法结合了微流体放大和大量并行的测序。所得数据将用于重建这些物种的进化史，并将与基于样本的映射结合以测试总体生物地理假设。作为这项研究的一部分开发的基因组标志物还将评估其在生命中的其他物种向其他物种的转移性，这可能会扩大所有植物系统主义者的方法学工具包。这项研究将为两名本科生提供指导机会，并将支持他们的专业发展，包括在全国会议上介绍他们的研究。通过持续的指导活动，还将提供额外的外展活动，以涉及K-12和本科生。